Molecular detection and genetic diversity of porcine circovirus type 3 in commercial pig farms in Xinjiang province, China
, , , , , , , , , , and | Dec 19, 2019
About this article
Published Online: Dec 19, 2019
Page range: 481 - 488
Received: May 07, 2019
Accepted: Nov 22, 2019
DOI: https://doi.org/10.2478/jvetres-2019-0071
Keywords
© 2019 Q. Mengfan et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1
Fig. 2
Genetic diversity of PCV3 strains circulating in commercial pig farms in Xinjiang province, China
| PCV3 strains | Group | Subgroup |
|---|---|---|
| CN/Xinjiang-SH6/2018 | 1 | 1.1 |
| CN/Xinjiang-TA36/2018 | 1 | 1.1 |
| CN/Xinjiang-AL5/2018 | 1 | 1.2 |
| CN/Xinjiang-UR22/2018 | 1 | 1.1 |
| CN/Xinjiang-YI7/2018 | 2 | 2.1 |
| CN/Xinjiang-CH29/2018 | 2 | 2.2 |
| CN/Xinjiang-AK16/2018 | 2 | 2.1 |
| CN/Xinjiang-KO17/2018 | 2 | 2.2 |
| CN/Xinjiang-KA2/2018 | 1 | 1.1 |
List of primer sequences used in this study
| Primer name | Nucleotide sequence | Position in reference | Product size |
|---|---|---|---|
| (5′→ 3′) | sequence | (bp) | |
| FP1 | CCGTAGAAGTCTGTCATTCCAG | 1383–1404 | |
| RP1 | AAGCCCTGGCACGCCAACCAC | 1796–1816 | 434 |
| FP2 | TTAGAGAACGGACTTGTAACGA | 1343–1364 | |
| RP2 | ATGAGACACAGAGCTATATTCAG | 1965–1987 | 645 |
GenBank accession numbers of PCV3 strains
| Strain | Accession number |
|---|---|
| 2164 | KX458235 |
| PCV3-US/MN2016 | KX898030 |
| PCV3-US/SD2016 | KX966193 |
| PCV3/CN/Fujian-5/2016 | KY075986 |
| PCV3/CN/Henan-13/2016 | KY075988 |
| PCV3/CN/Jiangxi-62/2016 | KY075989 |
| PCV3/CN/Chongqing-150/2016 | KY075992 |
| CN/Hubei-618/2016 | KY354039 |
| CCV-A | KY363870 |
| CCV-B | KY363871 |
| CCV-C | KY363872 |
| PCV3-China/GD2016 | KY418606 |
| PCV3/KU-1601 | KY996337 |
| PCV3/KU-1604 | KY996340 |
| PCV3/KU-1606 | KY996342 |
| PCV3/KU-1608 | KY996344 |
| PCV3/KU-1609 | KY996345 |
| P1705SCYC/2017 | MF063070 |
| 16R927/2016 | MF063071 |
| PCV3-BR/RS/6 | MF079253 |
| PCV3/CN/Jiangxi-B1/2017 | MF589107 |
| PCV3/CN/Jiangxi-S1/2017 | MF589133 |
| PCV3/CN/Guangdong-CH/2016 | MF589112 |
| PCV3/CN/Guangdong-X1/2016 | MF589118 |
| 309 | MF589652 |
| JX-1/CH/2017 | MF677838 |
| 1621_Italy_2017 | MF805719 |
| 4332-5_Denmark_2017 | MF805723 |
| 4332-7_Denmark_2017 | MF805724 |
| DE2.8 | MG014377 |
| DE19.15 | MG014367 |
| DE27.16 | MG014370 |
| PCV3/HU/Szerencs/2017 | MG595741 |
| PCV3-RU/TY17 | MG679916 |
| PCV3-JSXY-201701 | MG868940 |
| PCV3-HBWH-201703 | MG868941 |
| PCV3-SH-201705 | MG868945 |
| SD | MG947596 |
| PCV3-CN2018HLG-5 | MH277111 |
| PCV3-CN2018JL-1 | MH277112 |
| PCV3-CN2018LN-3 | MH277117 |
| COL/Cundinamarca2/2018 | MH327785 |
| CN/Xinjiang-AK16/2018 | MK562412 |
| CN/Xinjiang-AL5/2018 | MK562413 |
| CN/Xinjiang-CH29/2018 | MK562414 |
| CN/Xinjiang-KA2/2018 | MK562415 |
| CN/Xinjiang-KO17/2018 | MK562416 |
| CN/Xinjiang-SH6/2018 | MK562417 |
| CN/Xinjiang-TA36/2018 | MK562418 |
| CN/Xinjiang-UR22/2018 | MK562419 |
| CN/Xinjiang-YI7/2018 | MK562420 |
| 29160 | NC031753 |
Detection of PCV3 infection in different samples from commercial pig farms in Xinjiang province, China
| Clinical samples | Number of samples | Number of positive samples | Positive rate (%) of PCV3 |
|---|---|---|---|
| Lymph nodes | 79 | 29 | 36.71 (29/79) a |
| Spleen | 93 | 22 | 23.66 (22/93) a |
| Lung | 62 | 9 | 14.52 (9/62) b |
| Pleural effusion | 57 | 13 | 22.81 (13/57) a |
| Serum | 102 | 15 | 14.71 (15/102) b |
| Total | 393 | 88 | 22.39 (88/393) |