Molecular detection and genetic diversity of  type 3 in commercial pig farms in Xinjiang province, China

Qiao Mengfan; Wang Xifeng; Zhang Guowu; Meng Qingling; Qiao Jun; Wang Lixia; Cai Kuojun; Zhang Jinsheng; Zhang Zaichao; Yu Weiwei; Peng Yelong; Cai Xuepeng

Open Access

Molecular detection and genetic diversity of porcine circovirus type 3 in commercial pig farms in Xinjiang province, China

Journal of Veterinary Research

Volume 63 (2019): Issue 4 (December 2019)

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Page range: 481 - 488

Received: May 07, 2019

Accepted: Nov 22, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0071

Keywords
type 3, molecular detection, genetic diversity, gene, China

© 2019 Q. Mengfan et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Porcine circovirus type 3 (PCV3) is a newly discovered porcine circovirus. The molecular characteristics and genetic evolution of PCV3 in Xinjiang province, China still being unclear, the aim of the study was their elucidation.

Material and Methods

A total of 393 clinical samples were collected from pigs on commercial farms in nine different regions of Xinjiang and phylogenetic analysis based on full-length Cap genes was performed.

Results

The prevalence at farm level was 100%, while in all the tested samples it was 22.39%. Nine PCV3 strains were detected in Xinjiang province and they shared 98.9–99.3% nucleotide and 97.5–100.0% Cap gene amino acid sequence identities with other epidemic strains from China and abroad. Compared with other epidemic strains of PCV3, there were 26 base mutation sites in the Cap gene in the nine Xinjiang strains, resulting in the mutation of amino acids at positions 20, 24, 75, 77, 108, 111 and 206. Phylogenetic analysis showed that these strains can be divided into two different genetic groups, to the first of which five strains affiliated and divided between subgroups 1.1 and 1.2, and to the second of which the other four strains affiliated and similarly divided between subgroups 2.1 and 2.2.

Conclusion

PCV3 circulates widely among commercial pig farms in Xinjiang province, China, and displays obvious genetic diversity. The results provide epidemiological information useful for the prevention and control of PCV3 infection in the pig industry.

eISSN:: 2450-8608
Language:: English

Publication timeframe:: 4 times per year
Journal Subjects:: Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine

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