Phenolic compounds, bioactive content and antioxidant capacity of the fruits of mulberry (Morus spp.) germplasm in Turkey

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The study was carried out in 2014 and 2015, and aimed to determine some important biochemical and antioxidant characteristics of the fruits of mulberry (Morus spp.) cultivars and genotypes found in Malatya (Turkey). Phenolic compounds (protocatechuic acid, vanillic acid, ellagic acid, rutin, quercetin, gallic acid, catechin, chlorogenic acid, caffeic acid, syringic acid, p-coumaric acid, o-coumaric acid, phloridzin and ferulic acid), organic acids, sugars, vitamin C and antioxidant capacity were analyzed in sampled fruits. The results showed that most of the biochemical content and antioxidant capacities of the cultivars and genotypes were significantly different from one another (p < 0.05). Among the phenolic compounds, rutin (118.23 mg 100 g-1), gallic acid (36.85 mg 100 g-1), and chlorogenic acid (92.07 mg 100 g-1) were determined to have the highest values for most of the fruit samples. Malic acid and citric acid were dominant among the organic acids for all the cultivars and genotypes except 44-Nrk-05. Glucose was measured as a more abundant sugar than fructose and sucrose in all samples. Antioxidant capacity, on the other hand, varied between 6.17 and 21.13 μmol TE g-1 among the cultivars and genotypes analyzed.

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  • Abraham G.E. Flechas J.D. 1992. Management of fibromyalgia: rationale for the use of magnesium and malic acid. J. Nutr. Med. 3: 49-59.

  • Bevilacqua A.E. Califano A.N. 1989. Determination of organic acids in dairy products by high performance liquid chromatography. J. Food Sci. 54: 1076-1079.

  • Butt M.S. Nazir A. Sultan M.T. Schroën K. 2008. Morus alba L. nature’s functional tonic. Trends Food Sci. Technol. 19: 505-512.

  • Çam M. Hişil Y. 2004. Gıda flavonoidlerinin yüksek basınç sıvı kromatografisi ile analizi. Akademik Gıda Der. 8: 22-25 (in Turkish).

  • Canan I. Gundogdu M. Seday U. Oluk C.A. Karasahin Z. Eroglu E.C. Yazici E. Ünlü M. 2016. Determination of antioxidant total phenolic total carotenoid lycopene ascorbic acid and sugar contents of Citrus species and mandarin hybrids. Turk. J. Agric. For. 40: 894-899.

  • Cemeroglu B. 2007. Gıda Analizleri [Food Analysis]. Food Technology Society Publication Ankara Turkey 168-171 (in Turkish).

  • Cemeroğlu B. Acar J. 1986. Meyve ve sebze isleme teknolojisi. Gıda Tek. Der. 6: 29-30 (in Turkish). Ercisli S. 2004. A short review of the fruit germplasm resources of Turkey. Genet. Res. Crop Evol. 51: 419-435.

  • Ercisli S. Orhan E. 2007. Chemical composition of white (Morus alba) red (Morus rubra) and black (Morus nigra) mulberry fruits. Food Chem. 103: 1380-1384.

  • Ercisli S. Orhan E. 2008. Some physico-chemical characteristics of black mulberry (Morus nigra L.) genotypes from Northeast Anatolia region of Turkey. Sci. Hort. 116: 41-46.

  • Ercisli S. Tosun M. Duralija B. Voca S. Sengul M. Turan M. 2010. Phytochemical content of some black (Morus nigra L.) and purple (Morus rubra L.) mulberry genotypes. Food Technol. Biotechnol. 48(1): 102-106.

  • Eyduran S.P. Ercisli S. Akin M. Beyhan O. Gecer M.K. Eyduran E. Erturk Y.E. 2015. Organic acids sugars vitamin C antioxidant capacity and phenolic compounds in fruits of white (Morus alba L.) and black (Morus nigra L.) mulberry genotypes. J. Appl. Bot. Food Qual. 88: 134-138.

  • Gecer M.K. Akin M. Gundogdu M. Eyduran S.P. Ercisli S. Eyduran E. 2016. Organic acids sugars phenolic compounds and some horticultural characteristics of black and white mulberry accessions from Eastern Anatolia. Can. J. Plant Sci. 96: 27-33.

  • Gundogdu M. Muradoglu F. Sensoy R.I.G. Yilmaz H. 2011. Determination of fruit chemical properties of Morus nigra L. Morus alba L. and Morus rubra L. by HPLC. Sci. Hort. 132: 37-41.

  • Gungor N. Sengul M. 2008. Antioxidant activity total phenolic content and selected physicochemical properties of white mulberry (Morus alba L.) fruits. Int. J. Food Prop. 11: 44-52.

  • Hegedus A. Engel R. Abrankó L. Balogh E. Blázovics A. Hermán R. Halász J. Ercisli S. Pedryc A. Stefanovits-Bányai É. 2010. Antioxidant and antiradical capacities in apricot (Prunus armeniaca L.) fruits: variations from genotypes years and analytical methods. J. Food Sci. 75: C722-730.

  • Imran M. Khan H. Shah M. Khan R. Khan F. 2010. Chemical composition and antioxidant activity of certain Morus species. J. Zhejiang Univ. Sci. B. 11(12): 973-980.

  • Karacali I. 2012. Storage and marketing of horticultural products. Ege University Agricultural Faculty Publication No. 494 Izmir Turkey (in Turkish).

  • Koyuncu F. Cetinbas M. Ibrahim E. 2014. Nutritional constituents of wild-grown black mulberry (Morus nigra L.). J. Appl. Bot. Food Qual. 87: 93-96.

  • Lale H. Ozcagiran R. 1996. A study on pomological phenological and fruit quality characteristics of mulberry (Morus sp.) species. Derim. 13: 177-182 (in Turkish).

  • Mahmood T. Anwar F. Abbas M. Boyce M.C. Saari N. 2012. Compositional variation in sugars and organic acids at different maturity stages in selected small fruits from Pakistan. Int. J. Mol. Sci. 13(2): 1380-1392.

  • Melgarejo P. Salazar D.M. Artes F. 2000. Organic acids and sugars composition of harvested pomegranate fruits. Eur. Food Res. Technol. 211: 185-190.

  • Memon A.A. Memon N. Luthria D.L. Bhanger M.I. Pitafi A.A. 2010. Phenolic acids profiling and antioxidant potential of mulberry (Morus laevigata W. Morus nigra L. Morus alba L.) leaves and fruits grown in Pakistan. Pol. J. Food Nutr. Sci. 60: 25-32.

  • Mikulic-Petkovsek M. Schmitzer V. Slatnar A. Stampar F. Veberic R. 2012. Composition of sugars organic acids and total phenolics in 25 wild or cultivated berry species. J. Food Sci. 77: C1064-1070.

  • Orhan E. Ercisli S. 2010. Genetic relationships between selected Turkish mulberry genotypes (Morus spp.) based on RAPD markers. Genet. Mol. Res. 9: 2176-2183.

  • Ozgen M. Reese R.N. Tulio A.Z. Scheerens J.C. Miller A.R. 2006. Modified 22-Azino-bis-3- ethylbenzothiazoline-6-sulfonic Acid (ABTS) method to measure antioxidant capacity of selected small fruits and a comparison to Ferric Reducing Antioxidant Power (FRAP) and 22-Diphenyl-1- picrylhdrazyl (DPPH) methods. J. Agric. Food Chem. 54: 1151-1157.

  • Ozgen M. Serce S. Kaya C. 2009. Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus rubra fruits. Sci. Hort. 119: 275-279.

  • Penniston K.L. Steele T.H. Nakada S.Y. 2007. Lemonade therapy increases urinary citrate and urine volumes in patients with recurrent calcium oxalate stone formation. Urology 70(5): 856-860.

  • Rodriguez-Delgado M.A. Malovana S. Perez J.P. Borges T. Garcia Montelongo F.J. 2001. Separation of phenolic compounds by high-performance liquid chromatography with absorbance and fluorimetric detection. J. Chrom. A. 912: 249-257.

  • Rodriguez-Mateos A. Vauzour D. Krueger C.G. Shanmuganayagam D. Reed J. Calani L. Mena P. Del Rio D. Crozier A. 2014. Bioavailability bioactivity and impact on health of dietary flavonoids and related compounds: an update. Arch Toxicol. 88(10): 1803-1853.

  • Ruttanaprasert R. Banterng P. Jogloy S. Vorasoot N. Kesmala T. Kanwar R.S. Holbrook C.C. Patanothai A. 2014. Genotypic variability for tuber yield biomass and drought tolerance in Jerusalem artichoke germplasm. Turk. J. Agric. For. 38: 570-580.

  • Sanchez E.M. Calin-Sanchez A. Carbonell- Barrachina A.A. Melgarejo P. Hernandez F. Martinez-Nicolas J.J. 2014. Physicochemical characterization of eight Spanish mulberry clones: Processing and fresh market aptitudes. Int. J. Food Sci. Technol. 49: 477-483.

  • Sanchez-Salcedo E.M. Mena P. Garcia-Viguera C. Martinez J.J. Hernandez F. 2015. Phytochemical evaluation of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits a starting point for the assessment of their beneficial properties. J. Funct. Foods 12: 399-408.

  • Sorkheh K. Khaleghi E. 2016. Molecular characterization of genetic variability and structure of olive (Olea europaea L.) germplasm collection analyzed by agromorphological traits and microsatellite markers. Turk. J. Agric. For. 40: 583-596.

  • Soyer Y. Koca N. Karadeniz F. 2003. Organic acid profile of Turkish white grapes and grape juices. J. Food Comp. Anal. 16: 629-636.

  • TSI 2016. Turkish Statistical Institute Dynamic Examination Vegetative Production Statistics; cited on 29 July 2016.

  • Zadernowski R. Naczk M. Nesterowicz J. 2005. Phenolic acid profiles in some small berries. J. Agric. Food Chem. 53: 2118-2124.

  • Zorenc Z. Veberic R. Stampar F. Koron D. Mikulic-Petkovsek M. 2016. Changes in berry quality of northern highbush blueberry (Vaccinium corymbosum L.) during the harvest season. Turk. J. Agric. For. 40: 855-867.

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