Optimization of the extraction parameters of Aloe Vera polyphenols and study of antioxidant and antifungal activities: application to molds isolated from durum wheat

Imen Laib 1 , Fairouz Boubrik 1  und Malika Barkat 1
  • 1 Laboratoire BIOQUAL, Institut de la Nutrition, de l’Alimentation et des Technologies Agro-Alimentaires (I.N.A.T.A.A.), Université Frères Mentouri-Constantine 1, 25000, Constantine, Algeria


The main objectives of this work are to optimize the extraction parameters, to test the antioxidant activity of Aloe Vera extract and to study the impact of this extract on deteriorating molds of Algerian variety of wheat (CIRTA). The extraction was optimized by central composite design. Determination of the polyphenols, flavonoids, and proanthocyanidins was performed by using colorimetric assays. Identification and quantification of phenolic compounds were performed by RPHPLC-UV method. The antioxidant activity was tested by three methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,20-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and CUPRAC (Cupric reducing antioxidant capacity), the antifungal activity of Aloe Vera extract on isolated strains from durum wheat were tested by dilution in a solid medium method. The optimum of total phenolic got was1,044 x 104 µg GAE/g of dry extract. The extract is rich in polyphenols, flavonoids, and proanthocyanidins. The analysis of phenolic compounds of Aloe Vera by RP-HPLC-UV revealed seven phenolic compounds. Strong antioxidant activity was obtained for Aloe Vera extract. Purification and microscopic study of isolated strains gave the possibility of identifying four strains: Alternaria spp1, Alternaria spp2, Penicillium spp, and Aspergillus spp. the antifungal potential of Aloe Veravaries according to the fungal genera and the concentrations of extract used.

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  • [1]. Djermoun, A., La production céréalière en Algérie : les principales caractéristiques, Revue Nature et Technologie,2009, 1, 45-53

  • [2]. Scariot M. A.; Radünz,L.L.; Dionello, R.G.; Toni, J.R.; Mossi, A.J.; Reichert Júnior, F.W.,Quality of wheat grains harvested with different moisture contents and stored in hermetic and conventional system., Journal of Stored Products Research,2018, 75, 29-34

  • [3]. Kibar, H., Influence of storage conditions on the properties of wheat varieties, Journal of Stored Products Research,2015, 62, 8-15

  • [4]. Mohapatra, D.; Kumar, S.; Kotwaliwale, N.; Singh, K.K.,Critical factors responsible for fungi growth in stored food grains and non-Chemical approaches for their control, Industrial Crops & Products,2017, 108, 162–182

  • [5]. Nithya, U.; Chelladurai, V.; Jayas, D. S.; White, N.D.G., Safe storage guidelines for duram wheat, Journal of Stored Products Research,2011, 47, 328–33.

  • [6]. Tsehaye, H.; Brurberg, M.B.; Sundheim, L.; Assefa, D.; Tronsmo, A.; Tronsmo, A.M., Natural occurrence of Fusarium species and fumonisin on maize grains in Ethiopia, Eur. J. Plant Pathol.,2017, 147, 141–155.

  • [7]. Fleurat-Lessard, F.,Integrated management of the risks of stored grain spoilage by seedborne fungi and contamination by storage mould mycotoxins – An update, Journal of Stored Products Research,2017, 71, 22-40

  • [8]. Matthews, S.; Noli, E.; Demir, I.; Khajeh-Hosseini, M.; Wagner, M.H., Evaluation of seed quality: from physiology to international standardization, Seed Sci. Res.,2012, 22, S69-S73.

  • [9]. Villers, P., Food safety and aflatoxin control, J. Food Res,2017, 6, 38–49.

  • [10]. Kumar, N., Green leaf volatiles in management of storage fungi of chick pea (Cicer arietinum L.), Ann. Plant Prot. Sci.,2017, 25, 171–175.

  • [11]. Del Palacio, A.; Bettucci, L.;Pan, D., Fusarium and Aspergillus mycotoxins contaminating wheat silage for dairy cattle feeding in Uruguay, Braz J Microbiol., 2016, 47(4), 1000-1005.

  • [12]. Mohapatra, D.; Kumar, S.; Kotwaliwale, N.; Singh, K.K., Critical factors responsible for fungi growth in stored food grains and non-chemical approaches for their control, Ind. Crops and Prod.,2017, 108, 162-182.

  • [13]. WHO.Rapport de la Conférence internationale sur la gestiondes produits chimiques sur les travaux de sa troisième session, 2012. http://www.who.int/ipcs/saicm/saicm/fr/

  • [14]. Anžlovar, S.; Likar, M.; Dolenc Koce, J., Antifungal potential of thyme essential oil as a preservative for storage of wheat seeds, Acta Botan. Croa., 2017, 76, 64–71.

  • [15]. Salehi, B.; Albayrak, S.; Antolak, H.; Kręgiel, D.; Pawlikowska, E.; Sharifi-Rad, M.; Uprety, Y.; Tsouh Fokou, P.V.; Yousef, Z.; Amiruddin Zakaria, Z.; Varoni, E.M.; Sharopov, F.; Martins, N.; Iriti, M.; Sharifi-Rad, J., AloeGenus Plants: From Farm to Food Applications and Phytopharmacotherapy, Int. J. Mol. Sci.,2018, 19, 2843

  • [16]. Salehi, B.; Valussi, M.; Jugran, A.K.; Martorell, M.; Ramírez-Alarcón, K.; Stojanović-Radić, Z.Z.; Antolak, H.; Kr˛egiel, D.; Mileski, K.S.; Sharifi-Rad, M.; et al., Nepeta species: From farm to food applications and phytotherapy, Trends Food Sci. Technol.,2018, 80, 104–122.

  • [17]. Lawless, J.; Allan, J., The clinical composition of Aloe Vera. In: Aloe Vera: Natural Wonder Cure. London: Thorsons Publishing Ltd, 2000, 161-171.

  • [18]. Ferro,V.A.; Bradbury, F.; Cameron, P.; Shakir,E.; Rahman, S.R.; Stimson, W.H., In vitro susceptibilities of Shigella flexneri and Streptococcus pyogenes to inner gel of Aloe barbadensis Miller, Agents Chemother., 2003, 47, 1137-1139.

  • [19]. Radha, M.H.; Laxmipriya, N.P., Evaluation of biological properties and clinical effectiveness of Aloe Vera: A systematic review, Journal of Traditional and Complementary Medicine,2015,5, 21-26

  • [20]. Abdolshahi, A.; Naybandi-Atashi, S.; Heydari-Majd, M.; Salehi, B.; Kobarfard, F.; Ayatollahi, S.A.; Ata, A.; Tabanelli, G.; Sharifi-Rad, M.; Montanari, C.; et al., Antibacterial activity of some Lamiaceae species against Staphylococcus aureus in yoghurt-based drink (Doogh), Cell. Mol. Biol. (Noisy-le-Grand, France),2018, 64, 71–77

  • [21]. Laib, I.; Barkat, M., Optimization of Conditions for Extraction of Polyphenols and the Determination of the Impact of Cooking on Total Polyphenolic, Antioxidant, and Anticholinesterase Activities of Potato, Foods,2018, 7, 36.

  • [22]. Waterhouse, A.; Folin-Ciocalteu, Micro Method for Total Phenol in Wine, Food Anal. Chem.,1999, 299, 152-78

  • [23]. Woisky, R.; Salatino, A., Analysis of propolis: some parameters and procedures for chemical quality control, J. Apic. Res,1998, 37, 99-105

  • [24]. Skerget, M.; Kotnik, P.; Hadolin, M.; Hras, A.R.; Simonic, M.; Knez, Z., Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities, Food Chem.,2005, 89, 191–198

  • [25]. Blois, M.S., Antioxidant determinations by the use of a stable free radical, Nature,1958, 181, 1199–1200

  • [26]. Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C., Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med.,1999, 26, 1231.

  • [27]. Özyürek, M.; Güçlü, K.; Apak, R., The main andmodified CUPRAC methods of antioxidant measurement, TrAC-Trends Anal. Chem.,2011, 30, 652–664.

  • [28]. Pitt,J.I.; HockingaD., Fungi and food spoilage. Springer, London & New York, 1999, 501.

  • [29]. Gakuubi, M.M.; Maina, A.W.; Wagacha,J.M.,Antifungal Activity of Essential Oil of Eucalyptus camaldulensis Dehnh. against Selected Fusarium spp., International Journal of Microbiology,2017,2017, 7.

  • [30]. Kordali, S.; Cakir, A.; Zengin, H.; Duru, M. E., Antifungical Activities of The leaves of three Pistacia species grown in Turkey, Fitoterapia, 2003, 74, 164-167.

  • [31]. Nejatzadeh-Barandozi, F.,Antibacterial activities and antioxidant capacity of Aloe Vera., Organic and Medicinal Chemistry Letters,2013,. 3, 2191-2858.

  • [32]. Kumar, S.; Yadav, A.; Yadav, M.; Yadav, J.P.,Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidant activity of Aloe Vera (L.), Burm.f. BioMed Central., 2017, 10

  • [33]. Vastrad, J.V.; Goudar, G.; Byadgi S.A.; Devi, R.D.; Kotur, R., Identification of bio-active components in leaf extracts of Aloe Vera, Ocimum tenuiflorum(Tulasi) and Tinospora cordifolia (Amrutballi), J. Med. Plants Res., 2015, 9(28), 764-770.

  • [34]. Suhaj, M.,Spice antioxidants isolation and their antiradical activity: à review, Journal of Food Composition and Analysis,2006, 19, 531–537.

  • [35]. Muthukumaran, P.; Divya, R.; Indhumathi, E.; Keerthika, C.,Total phenolic and flavonoid content of membrane processed Aloe Veraextract: a comparative study, International Food Research Journal,2018, 25(4), 1450-1456.

  • [36]. Amoo, S.O.; Aremu, A.O.; Van Staden, J., In vitro plant regeneration, secondary metabolite production and antioxidant activity of micropropagated Aloe arborescens Mill., Plant Cell Tissue Organ Cult.,2012, 111, 345–358

  • [37]. López, A.; de Tangil, M.S.; Vega-Orellana, O.; Ramírez, A.S.; Rico, M., Phenolic Constituents, Antioxidant and Preliminary Antimycoplasmic Activities of Leaf Skin and Flowers of Aloe Vera (L.) Burm. f. (syn. A. barbadensis Mill.) from the Canary Islands (Spain), Molecules,2013, 18, 4942-4954.

  • [38]. Bhalla, A.; Chauhan, U.K.,Identification of Antihyperlipidemic components in Aloe Verathrough reverse phase HPLC, Journal of Biological Sciences and Medicine,2015,1, 21-27

  • [39]. Cock, I., The genus aloe: Phytochemistry and therapeutic uses including treatments for gastrointestinal conditions and chronic inflammation. In Novel Natural Products: Therapeutic Effects in Pain, Arthritis and Gastro-Intestinal Diseases; Springer: Berlin, Germany, 2015, 179–235.

  • [40]. Keyhanian, S.; Stahl-Biskup, E., Phenolic constituents in dried flowers of Aloe Vera (Aloe barbadensis) and their in vitro antioxidative capacity, Planta Med.,2007, 73, 599–602.

  • [41]. Okamura, N.; Asai, M.; Hine, N.; Yagi, A., High-performance liquid chromatographic determination of phenolic compounds in Aloe species, J. Chromatogr. A,1996, 746, 225–231.

  • [42]. Park, M.K.; Park, J.H.; Kim, N.Y.; Shin, Y.G.; Choi, Y.S.; Lee, J.G.; Kim, K.H.; Lee, S.K., Analysis of 13 phenolic compounds in Aloe species by high performance liquid chromatography, Phytochem. Anal.,1998, 9, 186–191.

  • [43]. Haritha, K.; Ramesh, B.; Saralakumari, D., Effect of Aloe Vera gel on antioxidant enzymes in streptozotocin-induced cataractogenesis in male and female Wistar rats, J Acute Med.,2014, 4(1),38–44

  • [44]. Lee, H.; Guo, Y; Ohta, M.; Xiong, L.; Stevenson, B.; Zhu, J.K., LOS2, a genetic locus acquired for cold responsive gene transcription encodes a bifunctional enolase, EMBO J.,2002, 21, 2692–702.

  • [45]. Hu, Y.; Xu, J.; Hu Q.,Evaluation of antioxidant potential of Aloe Vera(Aloe barbadensis Miller) extracts, J Agric Food Chem.,2003, 51(26), 7788.

  • [46]. Saritha M.,Efficacy of topical Aloe Verain patients with oral lichen planus: à randomized double-blind study, J Oral Pathology and Medicine,2010, 39 (10), 735–740.

  • [47]. Salawu, K.M.; Ajaiyeoba, E.O.; Ogbole, O.O.; Adeniji, J.A; Faleye, T.C.; Agunu, A., Antioxidant, brine shrimp lethality and antiproliferative properties of gel and leaf extracts of Aloe schweinfurthii and Aloe Vera, J Herbs Spices Med Plants,2017, 23(4), 263-27.

  • [48]. Pfohl-Leszkowicz, A.,Métabolisation des mycotoxines-Effets biologiques et pathologies-Ecotoxicogenèse. Dans Les mycotoxines dans l’alimentation : évaluation et gestion du risque. De Conseil Supérieur d’Hygiène Publique de France. Technique et Documentation, Paris, 1999, 18-35

  • [49]. Miller, J. D., Fungi and mycotoxins in grain: implications for stored product research, J. Stored Prod. Res,1995, 31, 1-16

  • [50]. Filtenborg, O.; Frisvad, J.C.; Thrane, U., Moulds in food spoilage, International Journal of Food Microbiology,1996,33, 85–102

  • [51]. Magan, N.; Cayley, G.R; Lacey, J., Effect of water activity and temperature on mycotoxin production by Alternaria alternate in culture and on wheat grain, Applied and Environmental Microbiology,1984, 47, 1113-1117

  • [52]. Patriarca, A.; Azcarate, M. P.; Terminiello, L.; Fernandez Pinto, V., Mycotoxin production by Alternaria strains isolated from Argentinean wheat, Int. J. Food Microbiol.,2007, 119, 219–222.

  • [53]. Castegnaro, M.; Pfohl-Leszkowicz, A., Les mycotoxines : contaminants omniprésents dans l’alimentation animale et humaine, dans La sécurité alimentaire du consommateur, Lavoisier, Tec&Doc, 2002.

  • [54]. Botton,B.; Bertron, A.; Fevere, M.; Gauthier, S.; Guph, D.; Larpent, J.P.; Reymond, P.; Sanglier, J.J.; Vaysser, Y.; Veau, S.,Moisissures utiles et nuisibles importance industrielle. 2ème Edition Masson collection biotechnologies, 1990, 5-10

  • [55]. Pitt, J.I.; Miscamble, B.F., Water relations of Aspergillus flavus and closely related species, Journal of Food Protection,1995, 58, 86-90.

  • [56]. Joshaghani, H.; Namjoo, M.; Rostami, M.; Kohsar, F.; Niknejad, F., Mycoflora of fungal contaminationin wheat storage (silos) in Golestan Province, North of Iran. Jundishapur, Journal of Microbiology,2013, 6(4), e6334

  • [57]. Ennouari, A.; Sanchis, V.; Rahouti, M.; Zinedine,A.,Isolation and molecular identification of mycotoxin producing fungi in durum wheat from Morocco, J. Mater. Environ. Sci.,2018, 9, 1470–1479

  • [58]. Anžlovar, S.; Likar, M.; Koce, J.D., Antifungal potential of thyme essential oil as a preservative for storage of wheat seeds, Acta Bot. Croat.,2017, 76, 64-71

  • [59]. Lorito, M.; Woo, S. L.; Garcia Fernandez, I.; Colucci, G.; Harman, G. E.; Pintor-Toro, J. A.; Filippone, E.; Muccifora, S.; Lawrence, C. B.; Zoina, A.; Tuzun, S.; Scala, F., Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens, Proc. Natl. Acad. Sci,. USA,1998, 95, 7860-7865.

  • [60]. Saks, Y.; Barkai-Golan, R., Aloe Vera gel activity against plant pathogenic fungi, Postharvest Biol. Technol.,1995, 6, 159–165

  • [61]. Pereira, A.P.; Ferreira, I.C.; Marcelino, F.; Valentão, P.; Andrade, P.B.; Seabra, R.; Estevinho, L.; Bento, A.; Pereira, J.A., Phenolic Compounds and Antimicrobial Activity of Olive (Olea europaea L. Cv. Cobrançosa) Leaves, Molecules,2007, 12, 1153-1162.

  • [62]. Dammak, I.; Lasram, S.; Hamdi, Z.; Ben Moussa, O.; Mkadmini Hammi, K.; Trigui, I.; Houissa, H.; Mliki, A.; Hassouna, M., In vitro antifungal and anti-ochratoxigenic activities of Aloe vera gel against Aspergillus carbonarius isolated from grape, Industrial Crops & Products,2018, 123, 416–423.

  • [63]. Harborne, J.B.; Williams, C.A., Advances in flavonoid research since 1992, Phytochemistry,2000, 55, 481–504.

  • [64]. Singh, M.; Kaur, M.; Silakari, O., Flavones: An important scaffold for medicinal chemistry, Eur. J. Med. Chem., 2014, 84, 206–239.

  • [65]. Nidiry, E.S.J.; Ganeshan G.; Lokesha, A.N.,Antifungal activity of some extractives and constituents of Aloe Vera.,Res. J. Med. Plant, 2011, 5, 196-200.

  • [66]. Bhat, T.K.; Singh, B.; Sharma, O.P., Microbial degradation of tannins – a current perspective, Biodegradation,1998, 9, 343–357

  • [67]. Castillejos, L.; Calsamiglia, S.; Ferret, A.,Effect of Essential Oil Active Compounds on Rumen Microbial Fermentation and Nutrient Flow in vitro systems, J Dairy Science,2006, 89, 2649-2658.


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