<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The main objectives of this work are to optimize the extraction parameters, to test the antioxidant activity of Aloe Vera extract and to study the impact of this extract on deteriorating molds of Algerian variety of wheat (CIRTA). The extraction was optimized by central composite design. Determination of the polyphenols, flavonoids, and proanthocyanidins was performed by using colorimetric assays. Identification and quantification of phenolic compounds were performed by RPHPLC-UV method. The antioxidant activity was tested by three methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,20-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and CUPRAC (Cupric reducing antioxidant capacity), the antifungal activity of Aloe Vera extract on isolated strains from durum wheat were tested by dilution in a solid medium method. The optimum of total phenolic got was1,044 x 104 µg GAE/g of dry extract. The extract is rich in polyphenols, flavonoids, and proanthocyanidins. The analysis of phenolic compounds of Aloe Vera by RP-HPLC-UV revealed seven phenolic compounds. Strong antioxidant activity was obtained for Aloe Vera extract. Purification and microscopic study of isolated strains gave the possibility of identifying four strains: Alternaria spp1, Alternaria spp2, Penicillium spp, and Aspergillus spp. the antifungal potential of Aloe Veravaries according to the fungal genera and the concentrations of extract used.</p></abstract>

The main objectives of this work are to optimize the extraction parameters, to test the antioxidant activity of Aloe Vera extract and to study the impact of this extract on deteriorating molds of Algerian variety of wheat (CIRTA). The extraction was optimized by central composite design. Determination of the polyphenols, flavonoids, and proanthocyanidins was performed by using colorimetric assays. Identification and quantification of phenolic compounds were performed by RPHPLC-UV method. The antioxidant activity was tested by three methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,20-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and CUPRAC (Cupric reducing antioxidant capacity), the antifungal activity of Aloe Vera extract on isolated strains from durum wheat were tested by dilution in a solid medium method. The optimum of total phenolic got was1,044 x 104 µg GAE/g of dry extract. The extract is rich in polyphenols, flavonoids, and proanthocyanidins. The analysis of phenolic compounds of Aloe Vera by RP-HPLC-UV revealed seven phenolic compounds. Strong antioxidant activity was obtained for Aloe Vera extract. Purification and microscopic study of isolated strains gave the possibility of identifying four strains: Alternaria spp1, Alternaria spp2, Penicillium spp, and Aspergillus spp. the antifungal potential of Aloe Veravaries according to the fungal genera and the concentrations of extract used.

Optimization of the extraction parameters of <italic>Aloe Vera</italic> polyphenols and study of antioxidant and antifungal activities: application to molds isolated from durum wheat

Laboratoire BIOQUAL, Institut de la Nutrition, de l’Alimentation et des Technologies Agro-Alimentaires (I.N.A.T.A.A.)

Université Frères Mentouri-Constantine 1

Acta Scientifica Naturalis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Optimization of the extraction parameters of  polyphenols and study of antioxidant and antifungal activities: application to molds isolated from durum wheat

The main objectives of this work are to optimize the extraction parameters, to test the antioxidant activity of Aloe Vera extract and to study the impact of...

Optimization of the extraction parameters of Aloe Vera polyphenols and study of antioxidant and antifungal activities: application to molds isolated from durum wheat

Published Online: May 27, 2019

Page range: 79 - 90

Received: Mar 01, 2019

Accepted: Apr 01, 2019

DOI: https://doi.org/10.2478/asn-2019-0011

Keywords
Optimization, Polyphenols, Antioxidant activity, Antifungal activity, Mold, wheat

© 2019 Imen Laib et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Optimization of the extraction parameters of Aloe Vera polyphenols and study of antioxidant and antifungal activities: application to molds isolated from durum wheat

Published Online: May 27, 2019

Page range: 79 - 90

Received: Mar 01, 2019

Accepted: Apr 01, 2019

DOI: https://doi.org/10.2478/asn-2019-0011

KeywordsOptimization, Polyphenols, Antioxidant activity, Antifungal activity, Mold, wheat

© 2019 Imen Laib et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Optimization, Polyphenols, Antioxidant activity, Antifungal activity, Mold, wheat