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Gravitational and Space Research
Édition 7 (2019): Edition 1 (August 2019)
Accès libre
Impact of
g
-Load Shift on Temporal Expression Pattern of Apoptosis-linked Proteins in the Rat Mammary Gland
Kibrom M. Alula
Kibrom M. Alula
,
James H. Resau
James H. Resau
et
Osman V. Patel
Osman V. Patel
| 17 juin 2019
Gravitational and Space Research
Édition 7 (2019): Edition 1 (August 2019)
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Article Category:
Short Communication
Publié en ligne:
17 juin 2019
Pages:
13 - 21
DOI:
https://doi.org/10.2478/gsr-2019-0003
Mots clés
Rat
,
Mammary Gland
,
Gravity
,
Apoptosis
,
Lactation
,
Pregnancy
,
Immunohistochemistry
© 2019 Kibrom M. Alula, James H. Resau, Osman V. Patel, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
Figure 1
Representative images of lobular outlining (green) and unmixing for protein quantification. An overlay image (a) was unmixed into blue hematoxylin (b) and red VDR (c). The unmixed images of dual-labeled slide enable quantification of individual, as well as overlapping signals within the same outlined lobular border. The scale bar represents 50 mm (10× magnification) in the photomicrographs. VDR, vitamin D receptor.
Figure 2
Quantitative comparison of mammary gland lobular CC-3 (a: within groups; b: between groups), P53 (c: within groups; d: between groups), and VDR (e: within groups; f: between groups) proteins measured in SC and HG-exposed rats following unmixing of chromophores using the CRi Nuance Multispectral Imaging System at G20, P1, and P3. Data are shown as mean ± SEM; *significantly different within and between each group (*p<0.05; **p<0.005; ***p<0.001; ****p<0.0001).G20, day 20 of gestation; HG, hypergravity; P1, postpartum day 1; P3, postpartum day 3; P53, tumor protein p53; SC, stationary control; SEM, standard error of the mean; VDR, vitamin D receptor.