Login
Register
Reset Password
Publish & Distribute
Publishing Solutions
Distribution Solutions
Subjects
Publications
Journals
Books
Proceedings
Publishers
Blog
Contact
Search
Cart
EUR
USD
GBP
English
English
Deutsch
Polski
Español
Français
Italiano
Home
Journals
Gravitational and Space Research
Volume 7 (2019): Issue 1 (August 2019)
Open Access
Impact of
g
-Load Shift on Temporal Expression Pattern of Apoptosis-linked Proteins in the Rat Mammary Gland
Kibrom M. Alula
Kibrom M. Alula
,
James H. Resau
James H. Resau
and
Osman V. Patel
Osman V. Patel
| Jun 17, 2019
Gravitational and Space Research
Volume 7 (2019): Issue 1 (August 2019)
About this article
Previous Article
Next Article
Abstract
Article
Figures & Tables
References
Authors
Articles in this Issue
Preview
PDF
Cite
Share
Article Category:
Short Communication
Published Online:
Jun 17, 2019
Page range:
13 - 21
DOI:
https://doi.org/10.2478/gsr-2019-0003
Keywords
Rat
,
Mammary Gland
,
Gravity
,
Apoptosis
,
Lactation
,
Pregnancy
,
Immunohistochemistry
© 2019 Kibrom M. Alula, James H. Resau, Osman V. Patel, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
Figure 1
Representative images of lobular outlining (green) and unmixing for protein quantification. An overlay image (a) was unmixed into blue hematoxylin (b) and red VDR (c). The unmixed images of dual-labeled slide enable quantification of individual, as well as overlapping signals within the same outlined lobular border. The scale bar represents 50 mm (10× magnification) in the photomicrographs. VDR, vitamin D receptor.
Figure 2
Quantitative comparison of mammary gland lobular CC-3 (a: within groups; b: between groups), P53 (c: within groups; d: between groups), and VDR (e: within groups; f: between groups) proteins measured in SC and HG-exposed rats following unmixing of chromophores using the CRi Nuance Multispectral Imaging System at G20, P1, and P3. Data are shown as mean ± SEM; *significantly different within and between each group (*p<0.05; **p<0.005; ***p<0.001; ****p<0.0001).G20, day 20 of gestation; HG, hypergravity; P1, postpartum day 1; P3, postpartum day 3; P53, tumor protein p53; SC, stationary control; SEM, standard error of the mean; VDR, vitamin D receptor.