<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.0904.424_s_001_w2aab3b7c11b1b6b1aab1c16b1Aa"><h3>Background</h3><p>Biofilm formation on indwelling devices is one of the most important mechanisms playing a role in device-related urinary tract infections caused by <italic>Proteus mirabilis</italic>. Increasing antibiotic resistance of microorganisms has raised questions concerning the relationship between biofilm formation and drug resistance.</p></sec><sec id="j_1905-7415.0904.424_s_002_w2aab3b7c11b1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To determine clinical prevalence, antibiotic resistance of, and biofilm formation by <italic>P. mirabilis</italic> strains.</p></sec><sec id="j_1905-7415.0904.424_s_003_w2aab3b7c11b1b6b1aab1c16b3Aa"><h3>Methods</h3><p>We studied the susceptibilities of various <italic>P. mirabilis</italic> strains isolated from different clinical materials by a disc-diffusion method. Biofilm formation was determined by a crystal violet binding assay.</p></sec><sec id="j_1905-7415.0904.424_s_004_w2aab3b7c11b1b6b1aab1c16b4Aa"><h3>Results</h3><p>Two (13%) of 15 <italic>P. mirabilis</italic> strains were found to be strong biofilm formers (SBF). Both SBF <italic>P. mirabilis</italic> strains were isolated from urine samples from children less than 15 years old in a pediatric emergency unit. Cefixime, cefazolin, ceftriaxone, amikacin, and piperacillin/tazobactam were the most effective antibiotics against 15 <italic>P. mirabilis</italic> strains (100%), whereas SBF <italic>P. mirabilis</italic> strains were multidrug resistant or (resistant to 5 different antimicrobial classes). Both of the SBF <italic>P. mirabilis</italic> strains, but neither of the weak biofilm forming <italic>P. mirabilis</italic> strains were resistant to ampicillin and ceftazidime among β-lactam antibiotics, or tobramycin and gentamicin among aminoglycoside antibiotics used in the present study.</p></sec><sec id="j_1905-7415.0904.424_s_005_w2aab3b7c11b1b6b1aab1c16b5Aa"><h3>Conclusions</h3><p>Children comprise the only patients infected with SBF <italic>P. mirabilis</italic> strains and both SBF P. mirabilis strains displayed high antimicrobial resistance in our setting.</p></sec></abstract>

BackgroundBiofilm formation on indwelling devices is one of the most important mechanisms playing a role in device-related urinary tract infections caused by Proteus mirabilis. Increasing antibiotic resistance of microorganisms has raised questions concerning the relationship between biofilm formation and drug resistance.ObjectivesTo determine clinical prevalence, antibiotic resistance of, and biofilm formation by P. mirabilis strains.MethodsWe studied the susceptibilities of various P. mirabilis strains isolated from different clinical materials by a disc-diffusion method. Biofilm formation was determined by a crystal violet binding assay.ResultsTwo (13%) of 15 P. mirabilis strains were found to be strong biofilm formers (SBF). Both SBF P. mirabilis strains were isolated from urine samples from children less than 15 years old in a pediatric emergency unit. Cefixime, cefazolin, ceftriaxone, amikacin, and piperacillin/tazobactam were the most effective antibiotics against 15 P. mirabilis strains (100%), whereas SBF P. mirabilis strains were multidrug resistant or (resistant to 5 different antimicrobial classes). Both of the SBF P. mirabilis strains, but neither of the weak biofilm forming P. mirabilis strains were resistant to ampicillin and ceftazidime among β-lactam antibiotics, or tobramycin and gentamicin among aminoglycoside antibiotics used in the present study.ConclusionsChildren comprise the only patients infected with SBF P. mirabilis strains and both SBF P. mirabilis strains displayed high antimicrobial resistance in our setting.

Multidrug resistance by biofilm-forming clinical strains of <italic>Proteus mirabilis</italic>

Faculty of Sciences, Department of Biology (Biotechnology)

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Multidrug resistance by biofilm-forming clinical strains of 

BackgroundBiofilm formation on indwelling devices is one of the most important mechanisms playing a role in device-related urinary tract infections caused by...

Multidrug resistance by biofilm-forming clinical strains of Proteus mirabilis

Article Category: Brief communication (Original)

Published Online: Jan 31, 2017

Page range: 535 - 541

DOI: https://doi.org/10.5372/1905-7415.0904.424

Keywords
Biofilm formation, device-related infections, multidrug resistance, Proteus mirabilis, urinary tract infection

© 2015 Gulcan Sahal, Isil Seyis Bilkay

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Multidrug resistance by biofilm-forming clinical strains of Proteus mirabilis

Article Category: Brief communication (Original)

Published Online: Jan 31, 2017

Page range: 535 - 541

DOI: https://doi.org/10.5372/1905-7415.0904.424

KeywordsBiofilm formation, device-related infections, multidrug resistance, Proteus mirabilis, urinary tract infection

© 2015 Gulcan Sahal, Isil Seyis Bilkay

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Biofilm formation, device-related infections, multidrug resistance, Proteus mirabilis, urinary tract infection