Sexually transmitted diseases in symptomatic and asymptomatic Thai women and girls: a study from Bangkok and nearby

Naraporn Somboonna; Ilada Choopara; Kanchapan Sukhonpan; Jarun Sayasathid

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Sexually transmitted diseases in symptomatic and asymptomatic Thai women and girls: a study from Bangkok and nearby

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| Jan 31, 2017

Volume 9 (2015): Issue 3 (June 2015)

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Published Online: Jan 31, 2017

Page range: 299 - 311

DOI: https://doi.org/10.5372/1905-7415.0903.398

Keywords
drug-resistant, human papillomavirus, sexually transmitted disease, tryptophan

© 2015 Naraporn Somboonna, Ilada Choopara, Kanchapan Sukhonpan, Jarun Sayasathid

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Neighbor-joining tree representing evolutionary relatedness of full-length ompA sequences from 19 C. trachomatis reference strains and 14 clinical isolates of serovars D, E, F, G, H, and K from Thai women and girls. All clinicals represent the isolates before antibiotic treatment. The length of branch is proportional to the distance between sequences, and number at node is the percent bootstrap confidence for clustering of strains.

Neighbor-joining tree representing evolutionary relatedness of full-length trpA sequences from 19 C. trachomatis reference strains and 14 clinical isolates from Thai women and girls (serovars D, E, F, G, H and K). All clinical isolates represent the first collected isolates prior to antibiotic treatment. Length of branch is proportional to distance between sequences, and number at node is the percent bootstrap confidence for clustering of strains.

Three-dimensional structures of non-active TrpA (A) and active TrpA–TrpB complex (B), with the nonsynonymous amino acid polymorphisms in respected to the clinical D/L11bf and H/U20bf (Q37R/R37Q, A115V/V115A, and C177Y). Clinical polymorphisms were represented in gray with atomic bonds, critical TrpA and TrpB residues (including C177Y) in gray, and TrpB COMM domain in white.

PCR primers and annealing temperatures

Primer name	Primer sequence (5′ → 3′)	Tm (°C)
Ct.ompA.60UF	GTCCCGCCAGAAAAAGATAG	45
Ct.ompA.VB3	CATCGTAGTCAATAGAGGCAT
Ct.ompA.MVF3	TGTAAAACGACGGCCAGTGCCCGTGCAGCTTTGTGGGAATGT	45
Ct.ompA.220DR	GCGCTCAAGTAGACCGATATAGTA
F.Ct.trpA	ATTAGCCACCGATGAAGAG	50
R.Ct.trpA	ATGTTGAATTAGGAGAGTTGTTAT
Ng.opa.F	TTGAAACACCGCCCGGAA	60
Ng.opa.R	TTTCGGCTCCTTATTCGGTTTAA
Ng.opa.R1	TTTCGGCTCCTTATTCGGTTTGA
Ng.opa.R2	TTTCGGCTCCTTATTCGGTTTGA
Ng.porA.F	CAGCATTCAATTTGTTCCGAGTC	60
Ng.porA.R	GAACTGGTTTCATCTGATTACTTTCCA
PGMY11-A	GCACAGGGACATAACAATGG	50
PGMY11-B	GCGCAGGGCCACAATAATGG
PGMY11-C	GCACAGGGACATAATAATGG
PGMY11-D	GCCCAGGGCCACAACAATGG
PGMY11-E	GCTCAGGGTTTAAACAATGG
PGMY09-F	CGTCCCAAAGGAAACTGATC
PGMY09-G	CGACCTAAAGGAAACTGATC
PGMY09-H	CGTCCAAAAGGAAACTGATC
PGMY09-I	GCCAAGGGGAAACTGATC
PGMY09-J	CGTCCCAAAGGATACTGATC
PGMY09-K	CGTCCAAGGGGATACTGATC
PGMY09-L	CGACCTAAAGGGAATTGATC
PGMY09-M	CGACCTAGTGGAAATTGATC
PGMY09-N	CGACCAAGGGGATATTGATC
PGMY09-P	GCCCAACGGAAACTGATC
PGMY09-Q	CGACCCAAGGGAAACTGGTC
PGMY09-R	CGTCCTAAAGGAAACTGGTC
HMB01	GCGACCCAATGCAAATTGGT
globinF	GAAGAGCCAAGGACAGGTAC	58
globinR	CAACTTCATCCACGTTCACC

Polymorphisms of C. trachomatis ompA and trpA among 14 Thai clinical isolates compared with the respective reference serovars

		ompA				trpA

Reference ompA genotype	Patient name	p-distance±S.E.	nt differences (type and position of changes)	dN/dS dN/dS represents the ratio of non-synonymous (dN) to synonymous (dS) amino acid changes (type and position of changes)	Serovars that the clinical sequence is most similar to If >1 serovars are most similar to the ompA or trpA sequence of the clinical isolate, the serovars are listed in alphabetical order (A, Ba, C, D, Da, E, F, G, H, I, Ia, J, Ja, K, L1, L2, L2a, L3) and bold for the respective reference serovar. For trpA analysis, serovar Β is excluded because of its lack of trpA	p-distance±S.E.	nt differences (type and position of changes)	dN/dS (type and position of dN changes)	Serovars that the clinical sequence is most similar to
	D/Ulbf	.000±.000	3 (G903A, A927QA937G) Y313C)	3/0(D301N, S309G	D	.000 ±.000	0	–	D, K
	D/U9bf	.000±.000	3 (G903A, A927QA937G)	3/0(D301N, S309G, Y313C)	D	.000 ±.000	0	–	D, K
D	D/U14bf	.006±.003	4(C258G, C594T, T601C, G603C)	0/4	D	.000 ±.000	0	^—	D, K
	D/Lllbf	.003 ± .002	2(C258C, G428C)	1/1 (S143T)	D	.005 ±.003	3(A110G, C344T, G530A)	3/0(Q37R, A115V, C177Y)	H, Ia, J, Ja
	E/U2bf	.000 ±.000	0	–	E	.000 ±.000	0	–	E
	E/U4bf	.000 ±.000	0	–	E	.000 ±.000	0	–	E
E	E/U28bf	.000 ±.000	0	–	E	.000 ±.000	0	–	E
	E/L8bf	.000 ±.000	0	–	E	.000 ±.000	0	–	E
F	F/U29bf	.000 ±.000	0	–	F	.000 ±.000	0	–	F, I
	F/Llbf	.000 ±.000	2(G769C, G775C)	2/0(R257T, S259T)	F	.000 ±.000	0	–	F, I
G	G/U18bf	.000 ±.000	0	–	G	.002 ±.002	1 (C10T)	0/1	F, I
	G/U3M	.000 ±.000	0	–	G	.002 ±.002	1 (C10T)	0/1	F, I
	H/U20bf	.000 ±.000	2(G795A, G885A)	2/0(G265R, W295.)	H	.004 ±.002	2(G110A, T344C)	2/0(R37Q, V115A)	F, I
H	H/L23bf	.000 ±.000	4(C270A, A271C, C850T, G885A)	4/0(N90H, T284I, W295.)	H	.000 ±.000	0	^—	H, Ia, J, Ja
K	K/U15bf	.000 ±.000	0	–	K	.000 ±.000	0	–	D,K

C. trachomatis serovar distribution and infection pattern after doxycycline treatment

Symptomatic	No. of patients (%)	Doxycycline treatment

		C. trachomatis		N. gonorrhoeae		HPV

		Before	After	Before	After	Before	After
Serovar D	6 (32)	6	2/4 The number after the solidus (/) represents the number of patients who did not come back after the treatment for C. trachomatis, N. gonorrhoeae, or HPV	0	0	3	1/2
Serovar E	4 (21)	4	1/3	0	0	4	1/3
Serovar F	4 (21)	4	1/3	1	0/1	4	1/3
Serovar G	2 (11)	2	0/2	0	0	1	0/1
Serovar H	2 (11)	2	0/2	0	0	1	0/1
Serovar K	1 (5)	1	0/1	0	0	0	0

Prevalence of STIs in symptomatic and asymptomatic urogenital tract females

Symptom location Symptom location includes UGT (upper urogenital tract), LGT (lower urogenital tract), Undefined (undefined UGT or LGT), and none for asymptomatic (n)	Ct Ct represents C. trachomatis (%)	Ng Ct represents C. trachomatis (%)	HPV (%)	Characterized by ages (%)	Ct Ng represents N. gonorrhoeae (%)	Ng Ng represents N. gonorrhoeae (%)	HPV (%)	Characterized by occupation (%)	Ct (%)	Ng (%)	HPV (%)
Symptomatic

UGT	18	4	35	15-24	12 (36)	2 (22)	18 (25)	Commercial	1	2	9
(57)	(12)	(3)	(23)	25-34	5 (15)	1 (11)	8 (11)	sex worker	(3)	(22)	(13)
				35-44	1 (3)	1 (11)	6 (8)
								Non-commercial	17	2	26
				45-54	0	0	1 (1)	sex worker	(52)	(22)	(36)
				N/A	0	0	2 (2)

LGT	14	5	29	15-24	4 (12)	0	11 (15)	Commercial	4	1	10
(75)	(9)	(3)	(19)	25-34	4 (12)	4 (44)	11 (15)	sex worker	(12)	(11)	(14)
				35-44	5 (15)	0	7 (10)	Non-commercial	10	4	19
				45-54	1 (3)	1 (11)	0	sex worker	(30)	(44)	(26)
				N/A	0	0	0

Undefined	1	0	8	15-24	0	0	1 (1)	Commercial	1	0	4
(18)	(1)		(5)	25-34	0	0	3 (4)	sex worker	(3)		(6)
				35-44	0	0	0	Non-
				45-54	0	0	0	commercial			(6)
				N/A	1 (3)	0	4 (6)	sex worker

Total	33	9	72	15-24	16 (48)	2 (22)	30 (42)	Commercial	6	3	23
(150)	(22)	(6)	(48)	25-34	9 (27)	5 (56)	22 (31)	sex worker	(18)	(33)	(32)
				35-44	6 (18)	1 (22)	13 (18)	Non-commercial	27	6	49
				45-54	1 (3)	1 (11)	1 (1)	sex worker	(82)	(67)	(68)
				N/A	1 (3)	0	6 (8)

Asymptomatic

None	4	21	14	15-24	0	7 (33)	6 (43)	Commercial	3	10	7
(134)	(3)	(16)	(10)	25-34	4 (100)	6 (29)	1 (7)	sex worker	(75)	(47.62)	(50)
				35-44	0	4 (19)	4 (29)	Non-commercial	1	11	7
				45-54	0	3 (14)	3 (21)	sex worker	(25)	(52.38)	(50)
				N/A	0	1 (5)	0

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Sexually transmitted diseases in symptomatic and asymptomatic Thai women and girls: a study from Bangkok and nearby

Article Category: Original article

Published Online: Jan 31, 2017

Page range: 299 - 311

DOI: https://doi.org/10.5372/1905-7415.0903.398

Keywords
drug-resistant, human papillomavirus, sexually transmitted disease, tryptophan

© 2015 Naraporn Somboonna, Ilada Choopara, Kanchapan Sukhonpan, Jarun Sayasathid

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

PCR primers and annealing temperatures

Polymorphisms of C. trachomatis ompA and trpA among 14 Thai clinical isolates compared with the respective reference serovars

C. trachomatis serovar distribution and infection pattern after doxycycline treatment

Prevalence of STIs in symptomatic and asymptomatic urogenital tract females

Sexually transmitted diseases in symptomatic and asymptomatic Thai women and girls: a study from Bangkok and nearby

Article Category: Original article

Published Online: Jan 31, 2017

Page range: 299 - 311

DOI: https://doi.org/10.5372/1905-7415.0903.398

Keywordsdrug-resistant, human papillomavirus, sexually transmitted disease, tryptophan

© 2015 Naraporn Somboonna, Ilada Choopara, Kanchapan Sukhonpan, Jarun Sayasathid

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

PCR primers and annealing temperatures

Polymorphisms of C. trachomatis ompA and trpA among 14 Thai clinical isolates compared with the respective reference serovars

C. trachomatis serovar distribution and infection pattern after doxycycline treatment

Prevalence of STIs in symptomatic and asymptomatic urogenital tract females

Keywords
drug-resistant, human papillomavirus, sexually transmitted disease, tryptophan