<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Bacterial urinary tract infection (UTI) is one of the most common patient problems that have no reliably definite diagnosis. The gold standard urine culture is usually delayed and increases laboratory workload. Objectives: To find a rapid method for the screening of bacterial urinary tract infection, the performance of four urinalysis parameters from automated urine dipstick test and flow cytometry were evaluated against the urine culture method. Methods: Urine cultures were performed on 372 routine specimens of suspected UTI. Urine dipstick tests were performed using an automated reader. An automated urine particle analyzer was used for quantitative counting of bacteria and white blood cells. Results: For screening of UTI, urinary bacteria count ≥14.2 cells/μL gave the highest sensitivity of 95.76% and 44.88% specificity. Conclusion: We suggest using urinary bacteria count for screening of UTI. This method can reduce the laboratory workload for processing urine culture by 30.64%.</abstract>

Background: Bacterial urinary tract infection (UTI) is one of the most common patient problems that have no reliably definite diagnosis. The gold standard urine culture is usually delayed and increases laboratory workload. Objectives: To find a rapid method for the screening of bacterial urinary tract infection, the performance of four urinalysis parameters from automated urine dipstick test and flow cytometry were evaluated against the urine culture method. Methods: Urine cultures were performed on 372 routine specimens of suspected UTI. Urine dipstick tests were performed using an automated reader. An automated urine particle analyzer was used for quantitative counting of bacteria and white blood cells. Results: For screening of UTI, urinary bacteria count ≥14.2 cells/μL gave the highest sensitivity of 95.76% and 44.88% specificity. Conclusion: We suggest using urinary bacteria count for screening of UTI. This method can reduce the laboratory workload for processing urine culture by 30.64%.

A flow cytometric urine analyzer for bacteria and white blood cell counts plus urine dipstick test for rapid screening of bacterial urinary tract infection

Division of Clinical Microscopy, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand

Division of Microbiology, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400,

Division of Clinical Microscopy, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Bacterial urinary tract infection (UTI) is one of the most common patient problems that have no reliably definite diagnosis. The gold standard...

A flow cytometric urine analyzer for bacteria and white blood cell counts plus urine dipstick test for rapid screening of bacterial urinary tract infection

Article Category: Brief communication (Original)

Published Online: Feb 04, 2017

Page range: 601 - 608

DOI: https://doi.org/10.5372/1905-7415.0604.097

Keywords
Flow cytometry, urinalysis, urine dipstick, UTI

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

A flow cytometric urine analyzer for bacteria and white blood cell counts plus urine dipstick test for rapid screening of bacterial urinary tract infection

Article Category: Brief communication (Original)

Published Online: Feb 04, 2017

Page range: 601 - 608

DOI: https://doi.org/10.5372/1905-7415.0604.097

KeywordsFlow cytometry, urinalysis, urine dipstick, UTI

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Flow cytometry, urinalysis, urine dipstick, UTI