<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">The pomegranate, <italic>Punica granatum</italic> L., has been the subject of current interest as a medicinal agent with wide-ranging therapeutic indications. In the present study, pomegranate ethanolic seed and hull extracts were tested, in comparison with a commercial sample, for the inhibition of aldose reductase, an enzyme involved in the etiology of diabetic complications. In vitro inhibition of rat lens aldose reductase was determined by a conventional method. Pomegranate ethanolic hull extract and commercial pomegranate hull extract exhibited similar aldose reductase inhibitory activity characterized by IC50 values ranging from 3 to 33.3 μg/ml. They were more effective than pomegranate ethanolic seed extract with IC50 ranging from 33.3 to 333 μg/ml. Antioxidant action of the novel compounds was documented in a DPPH test and in a liposomal membrane model, oxidatively stressed by peroxyl radicals. All the plant extracts showed considerable antioxidant potential in the DPPH assay. Pomegranate ethanolic hull extract and commercial pomegranate hull extract executed similar protective effects on peroxidatively damaged liposomal membranes characterized by 10 &lt; IC50 &lt; 100 μg/ml. Pomegranate ethanolic seed extract showed significantly lower antioxidant activity compared to both hull extracts studied. Pomegranate extracts are thus presented as bifunctional agents combining aldose reductase inhibitory action with antioxidant activity and with potential therapeutic use in prevention of diabetic complications.</abstract>

The pomegranate, Punica granatum L., has been the subject of current interest as a medicinal agent with wide-ranging therapeutic indications. In the present study, pomegranate ethanolic seed and hull extracts were tested, in comparison with a commercial sample, for the inhibition of aldose reductase, an enzyme involved in the etiology of diabetic complications. In vitro inhibition of rat lens aldose reductase was determined by a conventional method. Pomegranate ethanolic hull extract and commercial pomegranate hull extract exhibited similar aldose reductase inhibitory activity characterized by IC50 values ranging from 3 to 33.3 μg/ml. They were more effective than pomegranate ethanolic seed extract with IC50 ranging from 33.3 to 333 μg/ml. Antioxidant action of the novel compounds was documented in a DPPH test and in a liposomal membrane model, oxidatively stressed by peroxyl radicals. All the plant extracts showed considerable antioxidant potential in the DPPH assay. Pomegranate ethanolic hull extract and commercial pomegranate hull extract executed similar protective effects on peroxidatively damaged liposomal membranes characterized by 10 < IC50 < 100 μg/ml. Pomegranate ethanolic seed extract showed significantly lower antioxidant activity compared to both hull extracts studied. Pomegranate extracts are thus presented as bifunctional agents combining aldose reductase inhibitory action with antioxidant activity and with potential therapeutic use in prevention of diabetic complications.

Aldose reductase inhibitory activity and antioxidant capacity of pomegranate extracts

Cellular Stress Response & Signal Transduction Research Laboratory, Faculty of Medicine, Gazi University, Ankara, Turkey

Department of Pharmacognosy, Faculy of Pharmacy, Ankara University, Ankara, Turkey

Institute of Experimental Pharmacology & Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic

Interdisciplinary Toxicology

The pomegranate, Punica granatum L., has been the subject of current interest as a medicinal agent with wide-ranging therapeutic indications. In the present...

Aldose reductase inhibitory activity and antioxidant capacity of pomegranate extracts

Published Online: May 11, 2012

Page range: 15 - 20

DOI: https://doi.org/10.2478/v10102-012-0003-8

Keywords
Pomegranate, aldose reductase inhibition, antioxidant, diabetic complications

This content is open access.

Aldose reductase inhibitory activity and antioxidant capacity of pomegranate extracts

Published Online: May 11, 2012

Page range: 15 - 20

DOI: https://doi.org/10.2478/v10102-012-0003-8

KeywordsPomegranate, aldose reductase inhibition, antioxidant, diabetic complications

This content is open access.

Keywords
Pomegranate, aldose reductase inhibition, antioxidant, diabetic complications