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Hypothermia effects on liver and kidney oxidative stress parameters in an experimental model of sepsis in rats

Bogdan Sidonia
Bogdan Sidonia
, 
Rafa Horatiu
Rafa Horatiu
, 
Luca Vlad
Luca Vlad
, 
Dulf Francisc
Dulf Francisc
, 
Ober Ciprian
Ober Ciprian
, 
Pestean Cosmin
Pestean Cosmin
, 
Oana Liviu
Oana Liviu
 and 
Andrei Sanda
Andrei Sanda
   | Jan 20, 2020
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Published Online: Jan 20, 2020
Page range: 187 - 195
Received: May 30, 2019
Accepted: Jan 13, 2020
DOI: https://doi.org/10.2478/jvetres-2020-0004
Keywords
© 2020 B. Sidonia et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Total proteins concentration in the liver and kidneys for each experimental condition (C – control; SN – sepsis normothermic; SH– sepsis hypothermic). * – different from control (P < 0.05); ** – different from SN (p < 0.05). Values are expressed as means ± SD
Total proteins concentration in the liver and kidneys for each experimental condition (C – control; SN – sepsis normothermic; SH– sepsis hypothermic). * – different from control (P < 0.05); ** – different from SN (p < 0.05). Values are expressed as means ± SD

Fig. 2

GPx and SOD activity in the liver (A) and kidneys (B) for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic). * – different from control (p < 0.05); ** – different from SN (p < 0.05). Values are expressed as means ± SD
GPx and SOD activity in the liver (A) and kidneys (B) for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic). * – different from control (p < 0.05); ** – different from SN (p < 0.05). Values are expressed as means ± SD

Fig. 3

MDA and DNPH concentrations in the liver (A) and kidneys (B) for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic). * – different from control; ** – different from SN (p < 0.05). Values are expressed as means ± SD
MDA and DNPH concentrations in the liver (A) and kidneys (B) for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic). * – different from control; ** – different from SN (p < 0.05). Values are expressed as means ± SD

Fig. 4

GC-MS chromatogram of the FAMEs from total lipids of the liver (SH – sepsis hypothermic group) analysed with a BPx-70 column. Peaks of fatty acids: (1) palmitic (16:0); (2) palmitoleic (16:1 n–7); (3) stearic (18:0); (4) oleic (18:1 n–9); (5) linoleic (18:2 n–9); and (6) arachidonic (20:4 n–6)
GC-MS chromatogram of the FAMEs from total lipids of the liver (SH – sepsis hypothermic group) analysed with a BPx-70 column. Peaks of fatty acids: (1) palmitic (16:0); (2) palmitoleic (16:1 n–7); (3) stearic (18:0); (4) oleic (18:1 n–9); (5) linoleic (18:2 n–9); and (6) arachidonic (20:4 n–6)

Fig. 5

GC-MS chromatogram of the FAMEs from total lipids of kidneys (SN – sepsis normothermic group) analysed with a BPx-70 column. Peaks of fatty acids: (1) myristic (14:0); (2) palmitic (16:0); (3) palmitoleic (16:1 n–7); (4) stearic (18:0); (5) oleic (18:1 n–9); (6) linoleic (18:2 n–6); and (7) arachidonic (20:4 n–6)
GC-MS chromatogram of the FAMEs from total lipids of kidneys (SN – sepsis normothermic group) analysed with a BPx-70 column. Peaks of fatty acids: (1) myristic (14:0); (2) palmitic (16:0); (3) palmitoleic (16:1 n–7); (4) stearic (18:0); (5) oleic (18:1 n–9); (6) linoleic (18:2 n–6); and (7) arachidonic (20:4 n–6)

Statistical analysis of fatty acid distribution in total lipids for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic)

Fatty acid/% a
Type of tissues/ group Σ SFAs Σ MUFAs Σ PUFAs Σ UFAs Σ UFAs / Σ SFAs
C 44.63 ± 1.79 12.31 ± 0.46 43.06 ± 1.46 55.37 ± 2.13 1.24
Liver SN 54.17 ± 1.65* 8.19 ± 0.29* 37.63 ± 1.38* 50.19 ± 1.98* 0.84*
SH 49.82 ± 1.84*,** 10.98 ± 0.36*,** 39.19 ± 1.65 45.83 ± 1.73*,** 1.007**
C 7.87 ± 0.53 74.29 ± 2.93 17.84 ± 0.55 92.13 ± 3.69 11.70
Kidneys SN 32.42 ± 1.27* 57.59 ± 1.95* 9.99 ± 0.37* 67.58 ± 2.24* 2.08*
SH 10.48 ± 0.67*,** 72.00 ± 2.63** 17.52 ± 0.51 89.52 ± 3.33** 8.54**

Fatty acid composition of total lipids determined by GC-MS in the liver and kidneys for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic)

Fatty acid % a
Fatty acid C Liver SN SH C Kidneys SN SH
Myristic (14:0) nd nd nd 0.17 ± 0.09 1.01 ± 0.01 0.15 ± 0.01
Palmitic (16:0) 26.61 ± 1.21 24.48 ± 1.03 23.17 ± 0.98 7.17 ± 0.21 24.53 ± 1.06 9.71 ± 0.42
Palmitoleic (16:1 n–9) 0.16 ± 0.08 0.19 ± 0.06 0.17 ± 0.11 0.8 ± 0.09 0.21 ± 0.05 0.78 ± 1.01
Palmitoleic (16:1 n–7) 0.96 ± 0.12 0.92 ± 0.19 0.98 ± 0.11 0.26 ± 0.07 3.92 ± 0.24 nd
Stearic (18:0) 18.02 ± 1.13 29.69 ± 1.65 26.65 ± 1.16 0.53 ± 0.03 6.88 ± 0.29 0.62 ± 0.13
Oleic (18:1 n–9) 8.48 ± 0.32 5.15 ± 0.19 8.43 ± 0.28 69.22 ± 1.69 50.16 ± 1.53 67.62 ± 0.63
Oleic (18:1 n–7) 2.71 ± 0.13 1.94 ± 0.16 1.4 ± 0.09 4.01 ± 0.24 3.3 ± 0.52 3.59 ± 0.12
Linoleic (18:2 n–9) 14.12 ± 0.5 13.49 ± 0.29 12.88 ± 0.33 13.01 ± 0.31 9.85 ± 0.22 13.4 ± 0.52
Arachidonic (20:4 n–6) 28.94 ± 0.89 24.15 ± 0.91 26.32 ± 1.41 4.83 ± 0.12 0.14 ± 0.06 4.12 ± 0.16
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