<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2020-0004_s_006">
<h3>Introduction</h3>
<p>Oxidative stress (OS) seems to be an important mediator of cellular injury, from which sepsis can proceed. Studies have demonstrated the protective effect of controlled hypothermia in sepsis. This study aimed to evaluate its effects on OS parameters in rat hepatic and renal tissue septic after caecal ligation and puncture (CLP).</p></sec>
<sec id="j_jvetres-2020-0004_s_007">
<h3>Material and Methods</h3>
<p>Three groups were appointed (10 rats/group): C (control), SN (sepsis normothermic), and SH (sepsis hypothermic). Ten hours from CLP, the liver and kidneys were harvested and total protein concentration, superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, lipid peroxidation level (malondialdehyde (MDA), carbonylated proteins (2,4-dinitrophenylhydrazine (DNPH), and fatty acid profile were analysed.</p></sec>
<sec id="j_jvetres-2020-0004_s_008">
<h3>Results</h3>
<p>Sepsis significantly increased SOD and GPx activities in the liver, regardless of the temperature. In renal tissue, GPx activity increased significantly in normothermic conditions and SOD tended to decrease in hypothermic conditions. MDA and DNPH concentrations increase in both tissues after CLP. Hypothermia significantly lowered MDA in the liver but only changed it insignificantly in the kidneys. The DNPH in the liver and kidneys was significantly lower in hypothermic conditions. The unsaturated-to-saturated fatty acids ratio was significantly lower in sepsis, and the fall in temperature raised this ratio.</p></sec>
<sec id="j_jvetres-2020-0004_s_009">
<h3>Conclusion</h3>
<p>Experimentally induced sepsis in rats enhances OS in the liver and kidneys. The effect of hypothermia on OS indices is dependent on the type of tissue.</p></sec>
</abstract>

Introduction
Oxidative stress (OS) seems to be an important mediator of cellular injury, from which sepsis can proceed. Studies have demonstrated the protective effect of controlled hypothermia in sepsis. This study aimed to evaluate its effects on OS parameters in rat hepatic and renal tissue septic after caecal ligation and puncture (CLP).

Material and Methods
Three groups were appointed (10 rats/group): C (control), SN (sepsis normothermic), and SH (sepsis hypothermic). Ten hours from CLP, the liver and kidneys were harvested and total protein concentration, superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, lipid peroxidation level (malondialdehyde (MDA), carbonylated proteins (2,4-dinitrophenylhydrazine (DNPH), and fatty acid profile were analysed.

Results
Sepsis significantly increased SOD and GPx activities in the liver, regardless of the temperature. In renal tissue, GPx activity increased significantly in normothermic conditions and SOD tended to decrease in hypothermic conditions. MDA and DNPH concentrations increase in both tissues after CLP. Hypothermia significantly lowered MDA in the liver but only changed it insignificantly in the kidneys. The DNPH in the liver and kidneys was significantly lower in hypothermic conditions. The unsaturated-to-saturated fatty acids ratio was significantly lower in sepsis, and the fall in temperature raised this ratio.

Conclusion
Experimentally induced sepsis in rats enhances OS in the liver and kidneys. The effect of hypothermia on OS indices is dependent on the type of tissue.

Hypothermia effects on liver and kidney oxidative stress parameters in an experimental model of sepsis in rats

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Oxidative stress (OS) seems to be an important mediator of cellular injury, from which sepsis can proceed. Studies have demonstrated the...

Hypothermia effects on liver and kidney oxidative stress parameters in an experimental model of sepsis in rats

Published Online: Jan 20, 2020

Page range: 187 - 195

Received: May 30, 2019

Accepted: Jan 13, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0004

Keywords
rats, hypothermia, sepsis, oxidative stress

© 2020 B. Sidonia et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Hypothermia effects on liver and kidney oxidative stress parameters in an experimental model of sepsis in rats

Published Online: Jan 20, 2020

Page range: 187 - 195

Received: May 30, 2019

Accepted: Jan 13, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0004

Keywordsrats, hypothermia, sepsis, oxidative stress

© 2020 B. Sidonia et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
rats, hypothermia, sepsis, oxidative stress