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<p>This study aimed to evaluate the effect of biostimulants derived from <italic>Streptomyces avermitilis</italic> metabolites on entomopathogenic nematodes: <italic>Steinernema carpocapsae</italic>, <italic>Steinernema feltiae</italic>, and <italic>Heterorhabditis bacteriophora</italic>, obtained from the soil of several <italic>Miscanthus</italic> × <italic>giganteus</italic> plantations in 2020–2023. The nematodes were isolated, identified, and cultured using live insects (the greater wax moth <italic>Galleria mellonella</italic>). Three preparations containing biostimulants – Charkor, Stimpo, and Regoplant, were tested for compatibility with entomopathogenic and plant parasitic nematodes. Their effect on nematode survival was evaluated using the Petri dish test. The study showed that the effect of biostimulants on the survival of nematodes depended on the concentration of aversectin contained in the evaluated preparations. Stimpo and Regoplant had an adverse effect on plant parasitic nematodes. The highest <italic>G. mellonella</italic> mortality was observed at the higher dose of Charkor (0.4%), and the lowest at the lower dose of Regoplant (1.22%). The study showed that the virulence of the nematodes decreased after 48 hours of incubation in Regoplant and Stimpo solutions containing aversectin. The degree of interaction between nematodes depended on the nematode species, trophic preferences, and the concentration of active ingredients in the preparations. This conclusion is crucial for the selection of appropriate types of entomopathogenic nematodes and the application rate of plant parasitic nematodes in the assessment of their short- and long-term spread, persistence, and recycling in field conditions.</p>
</abstract>

This study aimed to evaluate the effect of biostimulants derived from Streptomyces avermitilis metabolites on entomopathogenic nematodes: Steinernema carpocapsae, Steinernema feltiae, and Heterorhabditis bacteriophora, obtained from the soil of several Miscanthus × giganteus plantations in 2020–2023. The nematodes were isolated, identified, and cultured using live insects (the greater wax moth Galleria mellonella). Three preparations containing biostimulants – Charkor, Stimpo, and Regoplant, were tested for compatibility with entomopathogenic and plant parasitic nematodes. Their effect on nematode survival was evaluated using the Petri dish test. The study showed that the effect of biostimulants on the survival of nematodes depended on the concentration of aversectin contained in the evaluated preparations. Stimpo and Regoplant had an adverse effect on plant parasitic nematodes. The highest G. mellonella mortality was observed at the higher dose of Charkor (0.4%), and the lowest at the lower dose of Regoplant (1.22%). The study showed that the virulence of the nematodes decreased after 48 hours of incubation in Regoplant and Stimpo solutions containing aversectin. The degree of interaction between nematodes depended on the nematode species, trophic preferences, and the concentration of active ingredients in the preparations. This conclusion is crucial for the selection of appropriate types of entomopathogenic nematodes and the application rate of plant parasitic nematodes in the assessment of their short- and long-term spread, persistence, and recycling in field conditions.

Effect of the Biostimulants of Microbiological Origin on the Entomopathogenic and Plant Parasitic Nematodes from <italic>M<sc>iscanthus</sc></italic> × <italic>G<sc>iganteus</sc></italic> Plantations

The National University of Life and Environmental Sciences

Jan Evangelista Purkyně University in Ústí nad Labem

Bogdan Khmelnytskyi Melitopol State Pedagogical University

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of the Biostimulants of Microbiological Origin on the Entomopathogenic and Plant Parasitic Nematodes from  ×  Plantations

This study aimed to evaluate the effect of biostimulants derived from Streptomyces avermitilis metabolites on entomopathogenic nematodes: Steinernema...

Effect of the Biostimulants of Microbiological Origin on the Entomopathogenic and Plant Parasitic Nematodes from Miscanthus × Giganteus Plantations

Published Online: May 08, 2024

Page range: -

Received: Dec 01, 2023

Accepted: Mar 01, 2024

DOI: https://doi.org/10.2478/johr-2024-0003

Keywords
abamectin, aversectin,

© 2024 Tatyana Stefanovska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of the Biostimulants of Microbiological Origin on the Entomopathogenic and Plant Parasitic Nematodes from Miscanthus × Giganteus Plantations

Published Online: May 08, 2024

Page range: -

Received: Dec 01, 2023

Accepted: Mar 01, 2024

DOI: https://doi.org/10.2478/johr-2024-0003

Keywordsabamectin, aversectin,

© 2024 Tatyana Stefanovska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
abamectin, aversectin,