<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Toxocara canis</italic> of dogs and <italic>Toxocara vitulorum</italic> of cattle and buffalo are nematode parasites that cause serious economic and public health problems all over the world. This study aims to provide molecular data to identify and distinguish between <italic>Toxocara</italic> spp. from dogs, cattle and buffalo in Egypt. Moreover, constructing a phylogeny and phylogenetic relationships among these <italic>Toxocara</italic> spp. were performed through an analytic study of ATPase-6, a mitochondrial gene; 12S, small subunit ribosomal RNA gene and ITS-2, the second internal transcribed spacer nuclear ribosomal gene. <italic>T. vitulorum</italic> from cattle and buffalo were found to be almost identical. The ATPase- 6 and 12S regions showed 87.78 % and 90.38 % nucleotide similarity between <italic>T. canis</italic> and <italic>T. vitulorum</italic>, while for the ITS-2 region, only 78.38 % was found. Analysis of the three studied genes revealed that each <italic>Toxocara</italic> spp. has distinct molecular characteristics. Moreover, it was revealed that these genes, especially the ITS-2 gene, are useful and sensitive molecular markers for classifying and studying the phylogenetic analysis and relationships among closely related <italic>Toxocara</italic> spp. All sequences obtained in this study were registered in the GenBank under the accession numbers: MG214149 -MG214157.</p></abstract>

Toxocara canis of dogs and Toxocara vitulorum of cattle and buffalo are nematode parasites that cause serious economic and public health problems all over the world. This study aims to provide molecular data to identify and distinguish between Toxocara spp. from dogs, cattle and buffalo in Egypt. Moreover, constructing a phylogeny and phylogenetic relationships among these Toxocara spp. were performed through an analytic study of ATPase-6, a mitochondrial gene; 12S, small subunit ribosomal RNA gene and ITS-2, the second internal transcribed spacer nuclear ribosomal gene. T. vitulorum from cattle and buffalo were found to be almost identical. The ATPase- 6 and 12S regions showed 87.78 % and 90.38 % nucleotide similarity between T. canis and T. vitulorum, while for the ITS-2 region, only 78.38 % was found. Analysis of the three studied genes revealed that each Toxocara spp. has distinct molecular characteristics. Moreover, it was revealed that these genes, especially the ITS-2 gene, are useful and sensitive molecular markers for classifying and studying the phylogenetic analysis and relationships among closely related Toxocara spp. All sequences obtained in this study were registered in the GenBank under the accession numbers: MG214149 -MG214157.

Molecular characterization and phylogenetic analysis of <italic>Toxocara</italic> species in dogs, cattle and buffalo in Egypt

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Molecular characterization and phylogenetic analysis of  species in dogs, cattle and buffalo in Egypt

Toxocara canis of dogs and Toxocara vitulorum of cattle and buffalo are nematode parasites that cause serious economic and public health problems all over the...

Gene	M.W. (bp)
Gene	T. canis	T. vitulorum from cattle	T. vitulorum from buffalo
ATPase-6	804	828	828
12S	572	576	572
ITS-2	592	625	625

Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt

Published Online: May 23, 2020

Page range: 83 - 90

Received: May 20, 2019

Accepted: Dec 21, 2019

DOI: https://doi.org/10.2478/helm-2020-0013

Keywords
molecular characterization, PCR-mitochondrial gene- ribosomal gene, dogs, cattle, buffalo, Egypt

© 2020 O. A. Mahdy, W. M. Mousa, S. Z. Abdel-Maogood, S. M. Nader, S. Abdel-Radi, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Fig. 1

Plate 1

Plate 2

Plate 3

Plate 4

Characterization of the PCR products of the selected genes in the tested Toxocara spp. showing the M. W. of the bands for each gene.

Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt

Published Online: May 23, 2020

Page range: 83 - 90

Received: May 20, 2019

Accepted: Dec 21, 2019

DOI: https://doi.org/10.2478/helm-2020-0013

Keywordsmolecular characterization, PCR-mitochondrial gene- ribosomal gene, dogs, cattle, buffalo, Egypt

© 2020 O. A. Mahdy, W. M. Mousa, S. Z. Abdel-Maogood, S. M. Nader, S. Abdel-Radi, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Fig. 1

Plate 1

Plate 2

Plate 3

Plate 4

Characterization of the PCR products of the selected genes in the tested Toxocara spp. showing the M. W. of the bands for each gene.

Keywords
molecular characterization, PCR-mitochondrial gene- ribosomal gene, dogs, cattle, buffalo, Egypt