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Journals
Gravitational and Space Research
Volume 6 (2018): Issue 1 (July 2018)
Open Access
Investigation of Zebrafish Larvae Behavior as Precursor for Suborbital Flights: Feasibility Study
Pedro J. Llanos
Pedro J. Llanos
,
Kristina Andrijauskaite
Kristina Andrijauskaite
,
Mark P. Rubinstein
Mark P. Rubinstein
and
Sherine S. L. Chan
Sherine S. L. Chan
| Jul 20, 2020
Gravitational and Space Research
Volume 6 (2018): Issue 1 (July 2018)
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Article Category:
Research Article
Published Online:
Jul 20, 2020
Page range:
37 - 57
DOI:
https://doi.org/10.2478/gsr-2018-0004
Keywords
Zebrafish Larvae Locomotor Motion
,
Suborbital
,
Physiological Stressors
,
Muscle Atrophy
© 2018 Pedro J. Llanos et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Figure 1
Distance traveled by zebrafish larvae during various time phases: 5 min light on (top), 1 min switch condition off where the light intensity is reduced from 100% light on to 100% light off (middle), 5 min light off (bottom). Each dot represents individual well of the 48-well plate. Colors correspond to the letters of the well: A comprises well numbers 1–8 with no zebrafish; B represents well numbers 9–16 with zebrafish in wells B2–B7; C indicates well numbers 17–24 with zebrafish in wells C2–C6; D corresponds to well numbers 25–32 with zebrafish in wells D2–D6; E comprises well numbers 33–40 with zebrafish in wells E2–E6; and F represents well numbers 41–48 with no zebrafish. Blue dots in wells C1, F4, and F6 are residues and do not reflect any motion.
Figure 2
Comparison of total distance traveled of zebrafish larvae during various light phases. 5 min light on is represented by the dashed line and 5 min light off is represented by the solid line. Color representations are as in Figure 1.
Figure 3
Behavioral motion of zebrafish larvae in B wells during different light conditions. The blue dots represent the distance traveled by larvae during the 5 min lighted condition. The red crosses denote the distance traveled by larvae during the switch condition. The black crosses correspond to the distance traveled by larvae under the 5 min dark condition.
Figure 4
Behavioral motion of zebrafish larvae in E wells during different light conditions. The blue dots represent the distance traveled by larvae during the 5 min lighted condition. The red crosses denote the distance traveled by larvae during the switch condition. The black crosses correspond to the distance traveled by larvae under the 5 min dark condition.
Figure 5
Behavioral motion of zebrafish larvae in C wells during different light conditions. The blue dots represent the distance traveled by larvae during the 5 min lighted condition. The red crosses denote the distance traveled by larvae during the switch condition. The black crosses correspond to the distance traveled by larvae under the 5 min dark condition.
Figure 6
Behavioral motion of zebrafish larvae in D wells during different light conditions. The blue dots represent the distance traveled by larvae during the 5 min lighted condition. The red crosses denote the distance traveled by larvae during the switch condition. The black crosses correspond to the distance traveled by larvae under the 5 min dark condition.
Figure 7
Behavioral motion of zebrafish larvae exposed to different temperature variations: 32°C (top), 28.5°C (middle), 25ºC (bottom). Each red dot represents the position for each discrete data point tracked by Ethovision®. The blue line indicates the approximated path of the zebrafish between data points.
Figure 8
Distance traveled by zebrafish larvae when exposed to an 11 min varying light cycle. (A) 3 min in light. (B) 1 min light to dark. (C) 3 min in dark. (D) 1 min dark to light. (E) 3 min in light.
Figure 9
Graphical representation of the zebrafish exposure to centrifugation profile. Left: Angular velocity as function of time. Right: G-force as function of time.
Figure 10
Representation of zebrafish after centrifugation. Left: Majority of zebrafish larvae localized in the bottom of the conical tube, whereas very few were swimming in the mid-top sections of the tube. Right: Magnification of the picture on the left showing some zebrafish swimming with their tails down or upside down.
Figure 11
Motion of zebrafish after exposure to centrifugation: no centrifugation (above), centrifugation (below). Green regions depict areas where the larvae did not swim (forbidden regions). X and Y represent the coordinates of each well on the 48-well plate.
Figure 12
Contour maps for radial and tangential velocities at various temperatures in different wells: (A) Well C4 at 32ºC. (B) Well C8 at 32ºC. (C) Well E1 at 28.5 ºC. (D) Well E5 at 28.5ºC. (E) Well D3 at 25ºC. (F) Well D6 at 25ºC
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