<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The objective of this study was to use the “Screening test for antibiotic residues” (STAR) as a broad-spectrum detection method for antibiotic residues in poultry meat. The STAR method is a microbiological inhibition assay (a five plate test) where the development of inhibition zones (IZs) indicates the presence of antibiotic residues in meat samples. By using the STAR method, in a total of 13 poultry products providing 18 meat samples (14 muscle and 4 skin) and 18 corresponding juice samples, 11 out of the 18 samples were positive for containing antibiotic residues. Based on muscle alone (which is the matrix validated for use in the STAR method), 6 of the 14 muscle samples were positive for antibiotic residues. The STAR method as a screening technique proves advantageous as it is relatively easy to perform and of a low cost. Furthermore, the STAR method not only indicates the presence or absence of antibacterial substances, but simultaneously, a positive sample gives an indication of the antibiotic family present due to the use of five different bacterial test organisms. Families of antibiotics pre-identified due to positive samples in the results of this study include aminoglycosides (one out of 18), beta-lactams and sulphonamides (6 out of 18), and macrolides (5 out of 18). Such pre-identification of the antimicrobial families allows for a targeted confirmatory analysis. However, one could argue that the STAR method is laborious and time consuming. Overall, given the potential for false positive/negative results, further confirmatory method analysis of the samples must be performed to ensure that the results and conclusions drawn here are true.</p></abstract>

The objective of this study was to use the “Screening test for antibiotic residues” (STAR) as a broad-spectrum detection method for antibiotic residues in poultry meat. The STAR method is a microbiological inhibition assay (a five plate test) where the development of inhibition zones (IZs) indicates the presence of antibiotic residues in meat samples. By using the STAR method, in a total of 13 poultry products providing 18 meat samples (14 muscle and 4 skin) and 18 corresponding juice samples, 11 out of the 18 samples were positive for containing antibiotic residues. Based on muscle alone (which is the matrix validated for use in the STAR method), 6 of the 14 muscle samples were positive for antibiotic residues. The STAR method as a screening technique proves advantageous as it is relatively easy to perform and of a low cost. Furthermore, the STAR method not only indicates the presence or absence of antibacterial substances, but simultaneously, a positive sample gives an indication of the antibiotic family present due to the use of five different bacterial test organisms. Families of antibiotics pre-identified due to positive samples in the results of this study include aminoglycosides (one out of 18), beta-lactams and sulphonamides (6 out of 18), and macrolides (5 out of 18). Such pre-identification of the antimicrobial families allows for a targeted confirmatory analysis. However, one could argue that the STAR method is laborious and time consuming. Overall, given the potential for false positive/negative results, further confirmatory method analysis of the samples must be performed to ensure that the results and conclusions drawn here are true.

Broad Spectrum Detection of Antibiotic Residues in Poultry Meat by a Multi-Plate Assay

University of Veterinary Medicine and Pharmacy in Košice

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The objective of this study was to use the “Screening test for antibiotic residues” (STAR) as a broad-spectrum detection method for antibiotic residues in...

Broad Spectrum Detection of Antibiotic Residues in Poultry Meat by a Multi-Plate Assay

Published Online: Sep 28, 2019

Page range: 9 - 17

Received: May 04, 2019

Accepted: Jul 10, 2019

DOI: https://doi.org/10.2478/fv-2019-0022

Keywords
antibiotics, meat, residues, screening

© 2019 H. S. Watkins et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Broad Spectrum Detection of Antibiotic Residues in Poultry Meat by a Multi-Plate Assay

Published Online: Sep 28, 2019

Page range: 9 - 17

Received: May 04, 2019

Accepted: Jul 10, 2019

DOI: https://doi.org/10.2478/fv-2019-0022

Keywordsantibiotics, meat, residues, screening

© 2019 H. S. Watkins et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
antibiotics, meat, residues, screening