The aim of this study was to determine antibiotic resistance of bacteria isolated from sheep cheese “bryndza”. The cheese samples were obtained from five Slovak farms and different groups of bacteria isolated from cheese samples. Enterobacteriaceae and Lactobacillaceae were the most distributed bacterial families with other bacterial families as Enterococcaceae, Staphylococcaceae and Moraxellaceae were identified. Enterobacteriaceae were tested to cefepime (cephalosporins) and imipenem (carbapenems), Lactobacillaceae family were tested for resistance to vancomycin (glycosides) and to imipenem. Staphylococcaceae were tested for resistance against tigecycline (tetracycline) and chloramphenicol (amphenicols). Enterococcaceae family was tested against tigecycline and imipenem. Genus