Analysis of the Methylation Status of CpG Sites Within Cancer-Related Genes in Equine Sarcoids

Agrawal A., Murphy R.F., Agrawal D.K. (2007). DNA methylation in breast and colorectal cancers. Mod. Pathol., 20: 711–721.10.1038/modpathol.380082217464311Search in Google Scholar

Altamura G., Strazzullo M., Corteggio A., Francioso R., Roperto F., D ‘ Esposto M., Borzacchiello G. (2012). O(6)-methylguanine-DNA methyltransferase in equine sarcoids: molecular and epigenetic analysis. BMC Vet. Res., 8: 218.10.1186/1746-6148-8-218351246423140380Search in Google Scholar

Bogaert L., van Heerden M., de Cock H.E.V., Martens A., Chiers K. (2011). Molecular and immunohistochemical distinction of equine sarcoid from schwannoma. Vet. Pathol., 48: 737–741.10.1177/030098581037707020634412Search in Google Scholar

Bonazzi V.F., Nancarrow D.J., Stark M.S., Moser R.J., Boyle G.M., Aoude L.G., Schmidt C., Hayward N.K. (2011). Cross-platform array screening identifies COL1A2, THBS1, TNFRSF10D and UCHL1 as genes frequently silenced by methylation in melanoma. PLoS One, 6:e26121.10.1371/journal.pone.0026121319759122028813Search in Google Scholar

Boultwood J., Wainscoat J.S. (2007). Gene silencing by DNA methylation in haematological malignancies. Brit. J. Haematol., 138: 3–11.10.1111/j.1365-2141.2007.06604.x17489980Search in Google Scholar

Broström H. (1995). Equine sarcoids. A clinical and epidemiological study in relation to equine leucocyte antigens (ELA). Acta Vet. Scan., 36: 223–236.10.1186/BF0354769180954137484549Search in Google Scholar

Carvalho A.L., Jeronimo C., Kim M.M., Henrique R., Zhang Z., Hoque M.O., Chang S., Brait M., Nayak C.S., Jiang W.W., Claybourne Q., Tokumaru Y., Lee J., Goldenberg D., Garrett-Mayer E., Goodman S., Moon C.S., Koch W., Wes-tra W.H., Sidransky D., Califano J.A. (2008). Evaluation of promoter hypermethylation detection in body fluids as a screening/diagnosis tool for head and neck squamous cell carcinoma. Clin. Cancer Res., 14: 97–107.10.1158/1078-0432.CCR-07-072218172258Search in Google Scholar

Chambers G., Ellsmore V.A., O ‘ Brien P.M., Reid S.W.J., Love S., Campo M.S., Nasir L. (2003). The association of bovine papillomavirus with equine sarcoids. J. Gen. Virol., 84: 1055–1062.10.1099/vir.0.18947-012692268Search in Google Scholar

Chan M.W., Chan L.W., Tang N.L., Lo K.W., Tong J.H., Chan A.W., Cheung H.Y., Wong W.S., Chan P.S., Lai F.M., To K.F. (2003). Frequent hypermethylation of promoter region of RASSF1A in tumor tissues and voided urine of urinary bladder cancer patients. Int. J. Cancer., 104: 611–616.10.1002/ijc.1097112594816Search in Google Scholar

Chen K., Sawhney R., Khan M., Benninger M.S., Hou Z., Sethi S., Stephen J.K., Worsham M.J. (2007). Methylation of multiple genes as diagnostic and therapeutic markers in primary head and neck squamous cell carcinoma. Arch. Otolaryngol. Head Neck Surg., 133: 1131–1138.10.1001/archotol.133.11.113118025318Search in Google Scholar

Christiansen D.H., Andersen M.K., Pedersen-Bjergaard J. (2003). Methylation of p15INK4B is common, is associated with deletion of genes on chromosome arm 7q and predicts a poor prognosis in therapy-related myelodysplasia and acute myeloid leukemia. Leukemia, 17: 1813–1819.10.1038/sj.leu.240305412970781Search in Google Scholar

Das P.M., Singal R. (2004). DNA methylation and cancer. J. Clin. Oncol., 22: 4632–4642.10.1200/JCO.2004.07.15115542813Search in Google Scholar

Djos A., Martinsson T., Kogner P., Carén H. (2012). The RASSF gene family members RASSF5, RASSF6 and RASSF7 show frequent DNA methylation in neuroblastoma. Mol. Cancer, 11: 40.10.1186/1476-4598-11-40349326622695170Search in Google Scholar

Epperson E.D., Castleman W.L. (2017). Bovine papillomavirus DNA and S100 profiles in sarcoids and other cutaneous spindle cell tumors in horses. Vet. Pathol., 54: 44–52.10.1177/030098581665316927312364Search in Google Scholar

Esteller M. (2005). Aberrant DNA methylation as a cancer-inducing mechanism. Annu. Rev. Pharmacol. Toxicol., 45: 629–656.10.1146/annurev.pharmtox.45.120403.09583215822191Search in Google Scholar

Evron E., Umbricht C.B., Korz D., Raman V., Loeb D.M., Niranjan B., Buluwe-la L., Weitzman S.A., Marks J., Sukumar S. (2001). Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation. Cancer Res., 61: 2782–2787.Search in Google Scholar

Fendri A., Masmoudi A., Khabir A., Sellami-Boudawara T., Daoud J., Frik-ha M., Ghorbel A., Gargouri A., Mokdad-Gargouri R. (2009). Inactivation of RASS-F1A, RARβeta2 and DAP-kinase by promoter methylation correlates with lymph node metastasis in nasopharyngeal carcinoma. Cancer Biol. Ther., 8: 444–451.10.4161/cbt.8.5.768619221469Search in Google Scholar

Furuta J., Umebayashi Y., Miyamoto K., Kikuchi K., Otsuka F., Sugimura T., Ushijima T. (2004). Promoter methylation profiling of 30 genes in human malignant melanoma. Cancer Sci., 95: 962–968.10.1111/j.1349-7006.2004.tb03184.x15596045Search in Google Scholar

Gonzalez-Gomez P., Bello M.J., Alonso M.E., Amiñoso C., Lopez-Marin I., De Campos J.M., Isla A., Gutierrez M., Rey J.A. (2004). Promoter methylation status of multiple genes in brain metastases of solid tumors. Int. J. Mol. Med., 13: 93–98.10.3892/ijmm.13.1.93Search in Google Scholar

Guo H., Carlson J.A., Slominski A. (2012). Role of TRPM in melanocytes and melanoma. Exp. Dermatol., 21: 650–654.10.1111/j.1600-0625.2012.01565.x342276122897572Search in Google Scholar

Hoon D.S., Spugnardi M., Kuo C., Huang S.K., Morton D.L., Taback B. (2004). Profiling epigenetic inactivation of tumor suppressor genes in tumors and plasma from cutaneous melanoma patients. Oncogene, 23: 4014–4022.10.1038/sj.onc.1207505285646915064737Search in Google Scholar

Leakey T., Zielinski J., Siegfried R.N., Siegel E.R., Fan C.Y., Cooney C.A. (2008). A simple algorithm for quantifying DNA methylation levels on multiple independent CpG sites in bisulfite genomic sequencing electropherograms. Nucleic Acids Res., 36: e64.10.1093/nar/gkn210244181018480118Search in Google Scholar

Lee M.G., Kim H.Y., Byun D.S., Lee S.J., Lee C.H., Kim J.I., Chang S.G., Chi S.G. (2001). Frequent epigenetic inactivation of RASSF1A in human bladder carcinoma. Cancer Res., 61: 6688–6692.Search in Google Scholar

Li Q., Ahuja N., Burger P.C., Issa J.P. (1999). Methylation and silencing of the Thrombospondin-1 promoter in human cancer. Oncogene, 18: 3284–3289.10.1038/sj.onc.120266310359534Search in Google Scholar

Lindner D.J., Wu Y., Haney R., Jacobs B.S., Fruehauf J.P., Tuthill R., Borden E.C. (2013). Thrombospondin-1 expression in melanoma is blocked by methylation and targeted reversal by 5-Aza-deoxycytidine suppresses angiogenesis. Matrix Biol., 32: 123–132.10.1016/j.matbio.2012.11.010361507123202046Search in Google Scholar

Lunardi M., de Alcântara B.K., Otonel R.A., Rodrigues W.B., Alfieri A.F., Alfieri A.A. (2013). Bovine papillomavirus type 13 DNA in equine sarcoids. J. Clin. Microbiol., 51: 2167–2171.10.1128/JCM.00371-13369770723637294Search in Google Scholar

Maruya S., Issa J.P., Weber R.S., Rosenthal D.I., Haviland J.C., Lotan R., El-Naggar A.K. (2004). Differential methylation status of tumor-associated genes in head and neck squamous carcinoma: incidence and potential implications. Clin. Cancer Res., 10: 3825–3830.10.1158/1078-0432.CCR-03-037015173091Search in Google Scholar

Maruyama R., Toyooka S., Toyooka K.O., Harada K., Virmani A.K., Zochbauer-Muller S., Farinas A.J., Vakar-Lopez F., Minna J.D., Sagalowsky A., Czer-niak B., Gazdar A.F. (2001). Aberrant promoter methylation profile of bladder cancer and its relationship to clinicopathological features. Cancer Res., 61: 8659–8663.Search in Google Scholar

Melnikov A., Shrestha S., Yi Q., Replogle C., Borgia J., Bonomi P., Liptay M., Ugolini D., Neri M., Verri C., Sozzi G., Levenson V. (2014). Non-small cell lung cancer can be detected and its subtypes differentiated by a blood test of methylation in cell-free DNA from plasma. JSM Biomar., 1: 1003.Search in Google Scholar

Ogi K., Toyota M., Ohe-Toyota M., Tanaka N., Noguchi M., Sonoda T., Koha-ma G., Tokino T. (2002). Aberrant methylation of multiple genes and clinicopathological features in oral squamous cell carcinoma. Clin. Cancer Res., 8: 3164–3171.Search in Google Scholar

Oshimo Y., Nakayama H., Ito R., Kitadai Y., Yoshida K., Chayama K., Yasui W. (2003). Promoter methylation of cyclin D2 gene in gastric carcinoma. Int. J. Oncol., 23: 1663–1670.10.3892/ijo.23.6.1663Search in Google Scholar

R Development Core Team (2011). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org/Search in Google Scholar

Rohde C., Zhang Y., Reinhardt R., Jeltsch A. (2010). BISMA – fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences. BMC Bioinformatics, 11: 230.10.1186/1471-2105-11-230287769120459626Search in Google Scholar

Spugnardi M., Tommasi S., Dammann R., Pfeifer G.P., Hoon D.S. (2003). Epigenetic inactivation of RAS association domain family protein 1 (RASSF1A) in malignant cutaneous melanoma. Cancer Res., 63: 1639–1643.Search in Google Scholar

Strazzullo M., Corteggio A., Altamura G., Francioso R., Roperto F., D ‘ Esposi-to M., Borzacchiello G. (2012). Molecular and epigenetic analysis of the fragile histidine triad tumour suppressor gene in equine sarcoids. BMC Vet. Res., 8: 30.10.1186/1746-6148-8-30336146422424615Search in Google Scholar

Virmani A.K., Rathi A., Sathyanarayana U.G., Padar A., Huang C.X., Cunnig-ham H.T., Farinas A.J., Milchgrub S., Euhus D.M., Gilcrease M., Herman J., Minna J.D., Gazdar A. F. (2001). Aberrant methylation of the adenomatous polyposis coli (APC) gene promoter 1A in breast and lung carcinomas. Clin. Cancer Res., 7: 1998–2004.Search in Google Scholar