<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In the recent years, particular attention was given to the research aimed at optimizing the use of tumour epigenetic markers. One of the best known epigenetic changes associated with the process of carcinogenesis is aberrant DNA methylation. The aim of the present research was to evaluate the methylation profile of genes potentially important in the diagnosis and/or prognosis of equine sarcoids, the most commonly detected skin tumours in <italic>Equidae</italic>. The methylation status of potential promoter sequences of nine genes: <italic>APC</italic>, <italic>CCND2</italic>, <italic>CDKN2B</italic>, <italic>DCC</italic>, <italic>RARβ</italic>, <italic>RASSF1</italic>, <italic>RASSF5</italic>, <italic>THBS1</italic> and <italic>TRPM1</italic>, was determined using bisulfite sequencing polymerase chain reaction (BSP-CR). The results of this study did not reveal any changes in the level of DNA methylation in the analysed group of candidate genes between the tumour and healthy tissues. Despite numerous reports describing the aberrant methylation of the promoters of the analysed genes in human cancers, the data obtained did not confirm the existence of such relationships in the examined tumour tissues, which excludes the possibility of using these genes for the diagnosis of the equine sarcoid.</p></abstract>

In the recent years, particular attention was given to the research aimed at optimizing the use of tumour epigenetic markers. One of the best known epigenetic changes associated with the process of carcinogenesis is aberrant DNA methylation. The aim of the present research was to evaluate the methylation profile of genes potentially important in the diagnosis and/or prognosis of equine sarcoids, the most commonly detected skin tumours in Equidae. The methylation status of potential promoter sequences of nine genes: APC, CCND2, CDKN2B, DCC, RARβ, RASSF1, RASSF5, THBS1 and TRPM1, was determined using bisulfite sequencing polymerase chain reaction (BSP-CR). The results of this study did not reveal any changes in the level of DNA methylation in the analysed group of candidate genes between the tumour and healthy tissues. Despite numerous reports describing the aberrant methylation of the promoters of the analysed genes in human cancers, the data obtained did not confirm the existence of such relationships in the examined tumour tissues, which excludes the possibility of using these genes for the diagnosis of the equine sarcoid.

Analysis of the Methylation Status of CpG Sites Within Cancer-Related Genes in Equine Sarcoids

Department of Animal Molecular Biology, National Research Institute of Animal Production

Swiss Institute of Equine Medicine, ALP-Haras

Department of Emergency Medicine, University Hospital Bern

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In the recent years, particular attention was given to the research aimed at optimizing the use of tumour epigenetic markers. One of the best known epigenetic...

Analysis of the Methylation Status of CpG Sites Within Cancer-Related Genes in Equine Sarcoids

Published Online: Nov 02, 2018

Page range: 907 - 918

Received: Mar 13, 2018

Accepted: Jun 27, 2018

DOI: https://doi.org/10.2478/aoas-2018-0033

Keywords
equine sarcoids, horse, tumourigenesis, DNA methylation, BSPCR

© 2018 Ewelina Semik-Gurgul et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Analysis of the Methylation Status of CpG Sites Within Cancer-Related Genes in Equine Sarcoids

Published Online: Nov 02, 2018

Page range: 907 - 918

Received: Mar 13, 2018

Accepted: Jun 27, 2018

DOI: https://doi.org/10.2478/aoas-2018-0033

Keywordsequine sarcoids, horse, tumourigenesis, DNA methylation, BSPCR

© 2018 Ewelina Semik-Gurgul et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
equine sarcoids, horse, tumourigenesis, DNA methylation, BSPCR