<abstract xmlns="http://www.w3.org/1999/xhtml">Two-step targeted 2D planar chromatographic method (2DTLC) was used in the determination of ginkgolic acids in pharmaceuticals and dietary supplements. The choice of the extraction method and the separation technique was guided by the formulation type (capsule, tablet, tincture) with expected low amounts of ginkgolic acids in the analyzed herbal samples. Separation of ginkgolic acids C15:1 and C17:1 on HPTLC RP18 WF254s was preceded by its separation from the sample matrix on TLC Si60 F254s. Mobile phases consisted of acetonitrile/water/formic acid (80:20:1, <italic>V/V/V</italic>) and <italic>n</italic>-heptane/ethyl acetate/formic acid (20:30:1, <italic>V/V/V</italic>), resp. Identification of separated compounds was based on 2D-TLC co-chromatography with reference substances and off-line 2D-TLC x HPLC-DAD-ESI-MS analysis. Quantification of ginkgolic acids C15:1 and C17:1 was conducted densitometrically. Among the analyzed products, the presence of ginkgolic acids was confirmed only in herbal drugs containing 60 % ethanolic tinctures of <italic>Ginkgo biloba</italic> leaves. The use of TLC in the quantification of ginkgolic acids C15:1 and C17:1 in ginkgo extracts was described for the first time.</abstract>

Two-step targeted 2D planar chromatographic method (2DTLC) was used in the determination of ginkgolic acids in pharmaceuticals and dietary supplements. The choice of the extraction method and the separation technique was guided by the formulation type (capsule, tablet, tincture) with expected low amounts of ginkgolic acids in the analyzed herbal samples. Separation of ginkgolic acids C15:1 and C17:1 on HPTLC RP18 WF254s was preceded by its separation from the sample matrix on TLC Si60 F254s. Mobile phases consisted of acetonitrile/water/formic acid (80:20:1, V/V/V) and n-heptane/ethyl acetate/formic acid (20:30:1, V/V/V), resp. Identification of separated compounds was based on 2D-TLC co-chromatography with reference substances and off-line 2D-TLC x HPLC-DAD-ESI-MS analysis. Quantification of ginkgolic acids C15:1 and C17:1 was conducted densitometrically. Among the analyzed products, the presence of ginkgolic acids was confirmed only in herbal drugs containing 60 % ethanolic tinctures of Ginkgo biloba leaves. The use of TLC in the quantification of ginkgolic acids C15:1 and C17:1 in ginkgo extracts was described for the first time.

Application of targeted 2D planar chromatography in the control of ginkgolic acids in some herbal drugs and dietary supplements

Department of Pharmacognosy with Medicinal Plants Garden

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Two-step targeted 2D planar chromatographic method (2DTLC) was used in the determination of ginkgolic acids in pharmaceuticals and dietary supplements. The...

Application of targeted 2D planar chromatography in the control of ginkgolic acids in some herbal drugs and dietary supplements

Published Online: Jan 16, 2020

Page range: 201 - 213

Accepted: Apr 28, 2019

DOI: https://doi.org/10.2478/acph-2020-0004

Keywords
ginkgolic acids, herbal products, 2D planar chromatography, HPLC-DAD-ESI-MS, densitometry

© 2020 Piotr Migas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Application of targeted 2D planar chromatography in the control of ginkgolic acids in some herbal drugs and dietary supplements

Published Online: Jan 16, 2020

Page range: 201 - 213

Accepted: Apr 28, 2019

DOI: https://doi.org/10.2478/acph-2020-0004

Keywordsginkgolic acids, herbal products, 2D planar chromatography, HPLC-DAD-ESI-MS, densitometry

© 2020 Piotr Migas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
ginkgolic acids, herbal products, 2D planar chromatography, HPLC-DAD-ESI-MS, densitometry