<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Several genes, namely CD44, CD90, CD105 and PCNA may be important in differentiation of porcine mucosa cell cultures. These genes are, inter alia, responsible for cell adhesion to extracellular matrix and its constituent secretion, cytoskeleton organization, epithelial to mesenchymal transition or proper course of DNA replication. A total of 20 pubertal crossbred Landrace gilts bred on commercial farms were used to produce buccal mucosa cultures, which were harvested on the 7th, 15th and 30th day after initiation of the culture. Expression levels of CD44, CD90, CD105 and PCNA were evaluated employing Real-Time Quantitative Polymerase Chain Reaction. CD44, CD90 and PCNA showed an unchanged expression pattern. Expression of CD44 on day 7 was the highest of all factors measured. The greatest difference between the measurement on 7th and 30th day was found in the PCNA gene. These results broaden the understanding of the transcriptome changes in porcine buccal mucosa cells for the duration of in vitro cultivation. Nevertheless, it is very important to consider that the in vitro conditions do not fully reflect the changes taking place in the living organism. It appears that tissues of the oral cavity possess high regenerative potential, and constitute suitable model for wound healing investigation.</p><p><bold>Running title</bold>: Confirmation of differentiation clusters’ and endoglin markers preset in porcine buccal mucosa cells</p></abstract>

Several genes, namely CD44, CD90, CD105 and PCNA may be important in differentiation of porcine mucosa cell cultures. These genes are, inter alia, responsible for cell adhesion to extracellular matrix and its constituent secretion, cytoskeleton organization, epithelial to mesenchymal transition or proper course of DNA replication. A total of 20 pubertal crossbred Landrace gilts bred on commercial farms were used to produce buccal mucosa cultures, which were harvested on the 7th, 15th and 30th day after initiation of the culture. Expression levels of CD44, CD90, CD105 and PCNA were evaluated employing Real-Time Quantitative Polymerase Chain Reaction. CD44, CD90 and PCNA showed an unchanged expression pattern. Expression of CD44 on day 7 was the highest of all factors measured. The greatest difference between the measurement on 7th and 30th day was found in the PCNA gene. These results broaden the understanding of the transcriptome changes in porcine buccal mucosa cells for the duration of in vitro cultivation. Nevertheless, it is very important to consider that the in vitro conditions do not fully reflect the changes taking place in the living organism. It appears that tissues of the oral cavity possess high regenerative potential, and constitute suitable model for wound healing investigation.Running title: Confirmation of differentiation clusters’ and endoglin markers preset in porcine buccal mucosa cells

Confirmation of differentiation clusters’ and endoglin markers preset in porcine buccal mucosa cells

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Several genes, namely CD44, CD90, CD105 and PCNA may be important in differentiation of porcine mucosa cell cultures. These genes are, inter alia, responsible...

Confirmation of differentiation clusters’ and endoglin markers preset in porcine buccal mucosa cells

Published Online: Dec 30, 2020

Page range: 118 - 123

Received: Aug 19, 2020

Accepted: Sep 23, 2020

DOI: https://doi.org/10.2478/acb-2020-0015

Keywords
cluster of differentiation, endoglin, cell culture, sus scrofa

© 2020 Blanka Borowiec, Rut Bryl, Artur Bryja, Paul Mozdziak, Marta Dyszkiewicz-Konwińska, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Confirmation of differentiation clusters’ and endoglin markers preset in porcine buccal mucosa cells

Published Online: Dec 30, 2020

Page range: 118 - 123

Received: Aug 19, 2020

Accepted: Sep 23, 2020

DOI: https://doi.org/10.2478/acb-2020-0015

Keywordscluster of differentiation, endoglin, cell culture, sus scrofa

© 2020 Blanka Borowiec, Rut Bryl, Artur Bryja, Paul Mozdziak, Marta Dyszkiewicz-Konwińska, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cluster of differentiation, endoglin, cell culture, sus scrofa