<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Chloramphenicol muscle residue levels in rainbow trout were determined after oral administration of 84 μg kg-1d-1 of chloramphenicol for four days. Samples were taken one day before treatment and for 43 days after the treatment was over. Chloramphenicol was analysed using an in-house enzyme linked immunoassay (ELISA) validated against the criteria of the Commission Decision 2002/657/EC. Validation parameters confirmed that the method was appropriate for the detection of chloramphenicol at levels below the minimum required performance limit (MRPL) of 0.3 μg kg-1. The highest chloramphenicol levels were observed on the first day after the treatment had ended (144.3 μg kg-1). Elimination was significant over the first seven days; significant differences were detected between days 1 and 3 (p&lt;0.001), 3 and 5 (p&lt;0.001), and 5 and 7 (p&lt;0.05). Chloramphenicol levels dropped below MRPL to 0.17 μg kg-1 on day 9 after the end of treatment. From day 11 to 43, chloramphenicol residues were detectable in a range from 0.091 μg kg-1 (highest) to 0.011 μg kg-1 (lowest). Our results indicate that trout muscle tissue could be compliant with health requirements for consumption 10 days after withdrawal from chloramphenicol treatment.</abstract>

Chloramphenicol muscle residue levels in rainbow trout were determined after oral administration of 84 μg kg-1d-1 of chloramphenicol for four days. Samples were taken one day before treatment and for 43 days after the treatment was over. Chloramphenicol was analysed using an in-house enzyme linked immunoassay (ELISA) validated against the criteria of the Commission Decision 2002/657/EC. Validation parameters confirmed that the method was appropriate for the detection of chloramphenicol at levels below the minimum required performance limit (MRPL) of 0.3 μg kg-1. The highest chloramphenicol levels were observed on the first day after the treatment had ended (144.3 μg kg-1). Elimination was significant over the first seven days; significant differences were detected between days 1 and 3 (p<0.001), 3 and 5 (p<0.001), and 5 and 7 (p<0.05). Chloramphenicol levels dropped below MRPL to 0.17 μg kg-1 on day 9 after the end of treatment. From day 11 to 43, chloramphenicol residues were detectable in a range from 0.091 μg kg-1 (highest) to 0.011 μg kg-1 (lowest). Our results indicate that trout muscle tissue could be compliant with health requirements for consumption 10 days after withdrawal from chloramphenicol treatment.

Elimination of Chloramphenicol in Rainbow Trout Receiving Medicated Feed

Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia

Veterinary Office of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina

Archives of Industrial Hygiene and Toxicology

{"article-title":"Elimination of Chloramphenicol in Rainbow Trout Receiving Medicated Feed"}

Chloramphenicol muscle residue levels in rainbow trout were determined after oral administration of 84 μg kg-1d-1 of chloramphenicol for four days. Samples...

Elimination of Chloramphenicol in Rainbow Trout Receiving Medicated Feed

Published Online: Oct 04, 2011

Page range: 215 - 220

DOI: https://doi.org/10.2478/10004-1254-62-2011-2106

Keywords
antibiotik, deplecija, ELISA, ostaci, riječna riba

This content is open access.

Elimination of Chloramphenicol in Rainbow Trout Receiving Medicated Feed

Published Online: Oct 04, 2011

Page range: 215 - 220

DOI: https://doi.org/10.2478/10004-1254-62-2011-2106

Keywordsantibiotik, deplecija, ELISA, ostaci, riječna riba

This content is open access.

Keywords
antibiotik, deplecija, ELISA, ostaci, riječna riba