<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Objectives:</bold> Inflammation plays a crucial role in many of the metabolic abnormalities. The prototypic marker of inflammation is C-reactive protein (CRP), Nitric Oxide (NO), inducible nitric oxide synthase (iNOS) and their inhibition is considered a promising strategy to combat inflammation. Here, we report the anti-inflammatory mechanism of <italic>Carica papaya</italic> root aqueous extract in sodium arsenic-induced renal dysfunction.<bold>Methodology:</bold> Thirty-five rats were used for the experiments. Griess assay was used to evaluate the inhibitory effect of <italic>Carica papaya</italic> roots aqueous extract on the overproduction of nitric oxide (NO). ELISA was used to determine the level of pro-inflammatory markers including c-reactive protein (CRP). ELISA was used to analyze 8-OHdG. The inhibitory effect on the enzymatic activity of inducible nitric oxide synthase (iNOS), adenosine deaminase (ADA), malondialdehyde (MDA) was tested by enzyme activity assay kits.<bold>Results:</bold><italic>Carica papaya</italic> roots aqueous extract suppressed sodium arsenite-stimulated NO production and proinflammatory secretion, such as CRP. <italic>Carica papaya</italic> roots aqueous extract significantly (p &lt; 0.05) decrease the activities of iNOS, 8-OHdG, ADA and MDA.<bold>Conclusion:</bold> These results indicated that potent inhibition on CRP, NO, iNOS, ADA, 8-OHdG might constitute the anti-inflammatory mechanism of <italic>Carica papaya</italic> roots aqueous extract.</abstract>

Objectives: Inflammation plays a crucial role in many of the metabolic abnormalities. The prototypic marker of inflammation is C-reactive protein (CRP), Nitric Oxide (NO), inducible nitric oxide synthase (iNOS) and their inhibition is considered a promising strategy to combat inflammation. Here, we report the anti-inflammatory mechanism of Carica papaya root aqueous extract in sodium arsenic-induced renal dysfunction.Methodology: Thirty-five rats were used for the experiments. Griess assay was used to evaluate the inhibitory effect of Carica papaya roots aqueous extract on the overproduction of nitric oxide (NO). ELISA was used to determine the level of pro-inflammatory markers including c-reactive protein (CRP). ELISA was used to analyze 8-OHdG. The inhibitory effect on the enzymatic activity of inducible nitric oxide synthase (iNOS), adenosine deaminase (ADA), malondialdehyde (MDA) was tested by enzyme activity assay kits.Results:Carica papaya roots aqueous extract suppressed sodium arsenite-stimulated NO production and proinflammatory secretion, such as CRP. Carica papaya roots aqueous extract significantly (p < 0.05) decrease the activities of iNOS, 8-OHdG, ADA and MDA.Conclusion: These results indicated that potent inhibition on CRP, NO, iNOS, ADA, 8-OHdG might constitute the anti-inflammatory mechanism of Carica papaya roots aqueous extract.

Aqueous Extract of Carica Papaya Linn Roots Halts Sodium Arsenite-Induced Renal Inflammation through Inhibiting Adenosine Deaminase, 8-Hydroxy-2′-Deoxyguanosine, C-Reactive Protein and Inducible Nitric Oxide Synthase Activity

Phytomedicine, Biochemical Toxicology and Diabetes Research Group, Department of Biochemistry

Experimental and Applied Biomedical Research (EABR)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Objectives: Inflammation plays a crucial role in many of the metabolic abnormalities. The prototypic marker of inflammation is C-reactive protein (CRP), Nitric...

Aqueous Extract of Carica Papaya Linn Roots Halts Sodium Arsenite-Induced Renal Inflammation through Inhibiting Adenosine Deaminase, 8-Hydroxy-2′-Deoxyguanosine, C-Reactive Protein and Inducible Nitric Oxide Synthase Activity

Published Online: Dec 29, 2017

Page range: 323 - 330

Received: Apr 28, 2017

Accepted: Jun 14, 2017

DOI: https://doi.org/10.1515/sjecr-2017-0029

Keywords
Linn. roots, anti-inflammatory biomarkers, renal inflammation

© 2017 Oluwafemi Adeleke Ojo et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Aqueous Extract of Carica Papaya Linn Roots Halts Sodium Arsenite-Induced Renal Inflammation through Inhibiting Adenosine Deaminase, 8-Hydroxy-2′-Deoxyguanosine, C-Reactive Protein and Inducible Nitric Oxide Synthase Activity

Published Online: Dec 29, 2017

Page range: 323 - 330

Received: Apr 28, 2017

Accepted: Jun 14, 2017

DOI: https://doi.org/10.1515/sjecr-2017-0029

KeywordsLinn. roots, anti-inflammatory biomarkers, renal inflammation

© 2017 Oluwafemi Adeleke Ojo et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Linn. roots, anti-inflammatory biomarkers, renal inflammation