<abstract xmlns="http://www.w3.org/1999/xhtml">Taken into consideration that molecular and cellular mechanisms involved in cardiotoxicity are still not clear the aim of this study was to compare the production of oxidative stress parameters in the isolated rat heart between animals chronically treated with cisplatin and saline. The hearts of male Wistar albino rats (n = 24, 12 per group, age 8 weeks, body mass 250±50 g) were excised and perfused according to the Langendorff technique at gradually increased coronary perfusion pressures (40-120 cm H2O). We followed the production of superoxide anion radicals, hydrogen peroxide, and nitrites and also index of lipid peroxidation during the changes of coronary perfusion pressure (CPP) (from 40 to 120 cm H2O) in coronary venous effluent. Modifications CPP were performed in order to determined if oxidative stress is involved in coronary endothelium response in conditions of hypoxia (lower than 60 cm H2O) and hyperoxia (higher than 80 cm H2O).Based on the results of this research we can conclude that with enhancement of CPP the values of oxidative stress statistically increased. However, this increment is more prominent in control group as a result of preserved endothelium and its more powerful response to hyperoxia. On the other hand, damaged endothelium of cisplatin-treated animals had weaker response to hyperoxia, and also lower antioxidant capacity.</abstract>

Taken into consideration that molecular and cellular mechanisms involved in cardiotoxicity are still not clear the aim of this study was to compare the production of oxidative stress parameters in the isolated rat heart between animals chronically treated with cisplatin and saline. The hearts of male Wistar albino rats (n = 24, 12 per group, age 8 weeks, body mass 250±50 g) were excised and perfused according to the Langendorff technique at gradually increased coronary perfusion pressures (40-120 cm H2O). We followed the production of superoxide anion radicals, hydrogen peroxide, and nitrites and also index of lipid peroxidation during the changes of coronary perfusion pressure (CPP) (from 40 to 120 cm H2O) in coronary venous effluent. Modifications CPP were performed in order to determined if oxidative stress is involved in coronary endothelium response in conditions of hypoxia (lower than 60 cm H2O) and hyperoxia (higher than 80 cm H2O).Based on the results of this research we can conclude that with enhancement of CPP the values of oxidative stress statistically increased. However, this increment is more prominent in control group as a result of preserved endothelium and its more powerful response to hyperoxia. On the other hand, damaged endothelium of cisplatin-treated animals had weaker response to hyperoxia, and also lower antioxidant capacity.

The Effects of Chronic Administration of Cisplatin on Oxidative Stress in the Isolated Rat Heart

Department of Physiology, Faculty of Medical Sciences

Department of Pharmacy, Faculty of Medical Sciences

Department of General and Inorganic Chemistry, Faculty of Chemistry

Experimental and Applied Biomedical Research (EABR)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Taken into consideration that molecular and cellular mechanisms involved in cardiotoxicity are still not clear the aim of this study was to compare the...

The Effects of Chronic Administration of Cisplatin on Oxidative Stress in the Isolated Rat Heart

Published Online: Apr 11, 2018

Page range: 11 - 16

Received: Jan 23, 2017

Accepted: Jan 26, 2017

DOI: https://doi.org/10.1515/sjecr-2017-0003

Keywords
cisplatin, change of coronary perfusion pressure, isolated rat heart, oxidative stress

© 2018 Jelena Smigic et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Effects of Chronic Administration of Cisplatin on Oxidative Stress in the Isolated Rat Heart

Published Online: Apr 11, 2018

Page range: 11 - 16

Received: Jan 23, 2017

Accepted: Jan 26, 2017

DOI: https://doi.org/10.1515/sjecr-2017-0003

Keywordscisplatin, change of coronary perfusion pressure, isolated rat heart, oxidative stress

© 2018 Jelena Smigic et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cisplatin, change of coronary perfusion pressure, isolated rat heart, oxidative stress