<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The aim of the study was to explore the effect of enrofloxacin on production of selected cytokines by porcine peripheral blood mononuclear cells (PBMCs).<bold>Material and Methods:</bold> Twenty pigs (10 control and 10 experimental) were used in this research. Pigs from experimental group received enrofloxacin at therapeutic doses. Blood samples were collected before, during, and after treatment with enrofloxacin. PBMCs were incubated with or without lipopolysaccharide (LPS). Production of IL-6, IL-10, INF-γ, and TNF-α were determined by ELISA.<bold>Results:</bold> Administration of enrofloxacin to healthy pigs for 5 d induced a transient reduction of the PBMCs response to LPS in terms of IL-6 and TNF-α secretion. The concentration of IL-6 returned to the day 0 level shortly after treatment, while TNF-α production remained reduced for 10 d after the treatment. The production of IL-10 was not affected by enrofloxacin. The level of IFN-γ was below the detection limit of the tests.<bold>Conclusion:</bold> The results indicate that enrofloxacin administered <italic>in vivo</italic> in therapeutic doses has an immunomodulatory effect through its capacity to inhibit secretion of IL-6 and TNF-α by porcine PBMC stimulated by LPS.</abstract>

Introduction: The aim of the study was to explore the effect of enrofloxacin on production of selected cytokines by porcine peripheral blood mononuclear cells (PBMCs).Material and Methods: Twenty pigs (10 control and 10 experimental) were used in this research. Pigs from experimental group received enrofloxacin at therapeutic doses. Blood samples were collected before, during, and after treatment with enrofloxacin. PBMCs were incubated with or without lipopolysaccharide (LPS). Production of IL-6, IL-10, INF-γ, and TNF-α were determined by ELISA.Results: Administration of enrofloxacin to healthy pigs for 5 d induced a transient reduction of the PBMCs response to LPS in terms of IL-6 and TNF-α secretion. The concentration of IL-6 returned to the day 0 level shortly after treatment, while TNF-α production remained reduced for 10 d after the treatment. The production of IL-10 was not affected by enrofloxacin. The level of IFN-γ was below the detection limit of the tests.Conclusion: The results indicate that enrofloxacin administered in vivo in therapeutic doses has an immunomodulatory effect through its capacity to inhibit secretion of IL-6 and TNF-α by porcine PBMC stimulated by LPS.

Enrofloxacin decreases IL-6 and TNF-α production by lipopolysaccharide-stimulated porcine peripheral blood mononuclear cells

Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: The aim of the study was to explore the effect of enrofloxacin on production of selected cytokines by porcine peripheral blood mononuclear cells...

Enrofloxacin decreases IL-6 and TNF-α production by lipopolysaccharide-stimulated porcine peripheral blood mononuclear cells

Published Online: May 28, 2016

Page range: 189 - 193

Received: Jan 27, 2016

Accepted: May 10, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0028

Keywords
swine, fluoroquinolone, immunomodulation, lipopolysaccharide, cytokines

© 2016 Małgorzata Pomorska-Mól et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Enrofloxacin decreases IL-6 and TNF-α production by lipopolysaccharide-stimulated porcine peripheral blood mononuclear cells

Published Online: May 28, 2016

Page range: 189 - 193

Received: Jan 27, 2016

Accepted: May 10, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0028

Keywordsswine, fluoroquinolone, immunomodulation, lipopolysaccharide, cytokines

© 2016 Małgorzata Pomorska-Mól et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
swine, fluoroquinolone, immunomodulation, lipopolysaccharide, cytokines