<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> In the present study, some of the commercial fish farms located in the Black Sea region of Turkey, were screened for bacteria between 2012 and 2014.<bold>Material and Methods:</bold> The bacterial agents isolated from fish were identified by classical biochemical tests and the rapid diagnostic tests (API 20 E and API 20 Strep). All strains were further identified by sequencing of the 16S rRNA genes. The strains were also investigated for resistance to different antimicrobials by the disc diffusion method. Antibiotic resistance genes, including tetracycline <italic>(B),</italic> β-lactam (<italic>ampC, blaTEM, blaPSE</italic>), florfenicol (<italic>floR</italic>), erythromycine (<italic>ereA, ereB</italic>), sulphonamide (<italic>sulI, sulII</italic>), and trimethoprim (<italic>dhfr1</italic>) genes, were determined by the PCR method.<bold>Results:</bold><italic>Vibrio anguillarum, Vibrio fluvialis, Photobacterium damselae</italic> subsp. <italic>piscicida, Pseudomonas luteola</italic>, <italic>Lactococcus garvieae, Streptococcus iniae, Aeromonas hydrophila</italic>, and <italic>Yersinia ruckeri</italic> were isolated from marine and freshwater cultured fish. According to the results of disc diffusion, all isolates were sensitive to florfenicol, trimethoprim+sulfamethoxazole, oxitetracycline, and enrofloxacin, and resistant to lincomycin, penicillin G, and amoxicillin. Also, <italic>sulI, sulII</italic>, and <italic>floR</italic> resistance genes were detected in the bacteria.<bold>Conclusion:</bold> The results of the study open up the opportunity to perform further investigations which could determine the possible role of ARGs in fish pathogens.</abstract>

Introduction: In the present study, some of the commercial fish farms located in the Black Sea region of Turkey, were screened for bacteria between 2012 and 2014.Material and Methods: The bacterial agents isolated from fish were identified by classical biochemical tests and the rapid diagnostic tests (API 20 E and API 20 Strep). All strains were further identified by sequencing of the 16S rRNA genes. The strains were also investigated for resistance to different antimicrobials by the disc diffusion method. Antibiotic resistance genes, including tetracycline (B), β-lactam (ampC, blaTEM, blaPSE), florfenicol (floR), erythromycine (ereA, ereB), sulphonamide (sulI, sulII), and trimethoprim (dhfr1) genes, were determined by the PCR method.Results:Vibrio anguillarum, Vibrio fluvialis, Photobacterium damselae subsp. piscicida, Pseudomonas luteola, Lactococcus garvieae, Streptococcus iniae, Aeromonas hydrophila, and Yersinia ruckeri were isolated from marine and freshwater cultured fish. According to the results of disc diffusion, all isolates were sensitive to florfenicol, trimethoprim+sulfamethoxazole, oxitetracycline, and enrofloxacin, and resistant to lincomycin, penicillin G, and amoxicillin. Also, sulI, sulII, and floR resistance genes were detected in the bacteria.Conclusion: The results of the study open up the opportunity to perform further investigations which could determine the possible role of ARGs in fish pathogens.

Identification of bacterial pathogens and determination of their antibacterial resistance profiles in some cultured fish in Turkey

Central Fisheries Research Institute, 61250, Trabzon-Turkey

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: In the present study, some of the commercial fish farms located in the Black Sea region of Turkey, were screened for bacteria between 2012 and...

Identification of bacterial pathogens and determination of their antibacterial resistance profiles in some cultured fish in Turkey

Published Online: May 28, 2016

Page range: 141 - 146

Received: Nov 30, 2015

Accepted: May 18, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0020

Keywords
fish, bacteria, antibiotic resistance, resistance gene, Turkey

© 2016 Mustafa Türe et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification of bacterial pathogens and determination of their antibacterial resistance profiles in some cultured fish in Turkey

Published Online: May 28, 2016

Page range: 141 - 146

Received: Nov 30, 2015

Accepted: May 18, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0020

Keywordsfish, bacteria, antibiotic resistance, resistance gene, Turkey

© 2016 Mustafa Türe et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
fish, bacteria, antibiotic resistance, resistance gene, Turkey