<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> The immunoreactivity of haemagglutinin (HA) polypeptides of equine influenza virus was compared among the strains isolated in Poland, using H3 monoclonal antibody. A stronger signal in immunoblot reaction was observed for A/equi/Pulawy/2008 HA polypeptides compared to A/equi/Pulawy/2006, despite the fact that both strains are phylogenetically closely related and belong to Florida clade 2 of American lineage. The strongest signal, observed in the case of A/equi/Pulawy/2008, seemed to be connected with the presence of G135, I213, E379, and/or V530 instead of R135, M213, G379, and I530 present in A/equi/Pulawy/2006 HA sequence. This implies that point mutations within amino acid sequences of HA polypeptides of equine influenza virus may change their immunoreactivity even when they are not located within five basic antigenic sites.</p>
</abstract>

The immunoreactivity of haemagglutinin (HA) polypeptides of equine influenza virus was compared among the strains isolated in Poland, using H3 monoclonal antibody. A stronger signal in immunoblot reaction was observed for A/equi/Pulawy/2008 HA polypeptides compared to A/equi/Pulawy/2006, despite the fact that both strains are phylogenetically closely related and belong to Florida clade 2 of American lineage. The strongest signal, observed in the case of A/equi/Pulawy/2008, seemed to be connected with the presence of G135, I213, E379, and/or V530 instead of R135, M213, G379, and I530 present in A/equi/Pulawy/2006 HA sequence. This implies that point mutations within amino acid sequences of HA polypeptides of equine influenza virus may change their immunoreactivity even when they are not located within five basic antigenic sites.

Different pattern of haemagglutinin immunoreactivity of equine influenza virus strains isolated in Poland

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The immunoreactivity of haemagglutinin (HA) polypeptides of equine influenza virus was compared among the strains isolated in Poland, using H3 monoclonal...

Different pattern of haemagglutinin immunoreactivity of equine influenza virus strains isolated in Poland

Published Online: Dec 30, 2015

Page range: 463 - 466

Received: Aug 19, 2015

Accepted: Nov 25, 2015

DOI: https://doi.org/10.1515/bvip-2015-0068

Keywords
equine influenza virus, haemagglutinin, immunoreactivity, horses

© National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Different pattern of haemagglutinin immunoreactivity of equine influenza virus strains isolated in Poland

Published Online: Dec 30, 2015

Page range: 463 - 466

Received: Aug 19, 2015

Accepted: Nov 25, 2015

DOI: https://doi.org/10.1515/bvip-2015-0068

Keywordsequine influenza virus, haemagglutinin, immunoreactivity, horses

© National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
equine influenza virus, haemagglutinin, immunoreactivity, horses