<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Two <italic>in vitro</italic> methods were tested to establish their potential to predict the metabolizable energy (ME) content of forage legumes: the Tilley and Terry (TT) method and the pepsin-cellulase method (CM). Different samples of white clover (<italic>Trifolium repens</italic> L.), red clover (<italic>Trifolium pratense</italic> L.), kura clover (<italic>Trifolium ambiguum</italic> M. Bieb.), lucerne (<italic>Medicago sativa</italic> L.), and birdsfoot trefoil (<italic>Lotus corniculatus</italic> L.) were derived from field trials with several defoliation systems at two sites. The CM was more precise due to its repeatability within and between analysis runs, but eventually overestimated the ME contents of the samples, as it was shown for the standard samples with known <italic>in vivo</italic> digestibility. ME contents were found to be consistently higher based on CM, with a difference of up to 1.5 MJ ME/kg DM compared to TT. Although white clover was, in general, the species with the highest ME content, the influence of legume species over all cuts and defoliation systems was inconsistent. Such observations may influence the method of choice for ME estimation for large datasets.</p></abstract>

Two in vitro methods were tested to establish their potential to predict the metabolizable energy (ME) content of forage legumes: the Tilley and Terry (TT) method and the pepsin-cellulase method (CM). Different samples of white clover (Trifolium repens L.), red clover (Trifolium pratense L.), kura clover (Trifolium ambiguum M. Bieb.), lucerne (Medicago sativa L.), and birdsfoot trefoil (Lotus corniculatus L.) were derived from field trials with several defoliation systems at two sites. The CM was more precise due to its repeatability within and between analysis runs, but eventually overestimated the ME contents of the samples, as it was shown for the standard samples with known in vivo digestibility. ME contents were found to be consistently higher based on CM, with a difference of up to 1.5 MJ ME/kg DM compared to TT. Although white clover was, in general, the species with the highest ME content, the influence of legume species over all cuts and defoliation systems was inconsistent. Such observations may influence the method of choice for ME estimation for large datasets.

Using <italic>in vitro</italic> methods to estimate metabolizable energy content of five forage legumes harvested under different defoliation systems / Einsatz von <italic>in vitro</italic> Methoden zur Schätzung der umsetzbaren Energie in fünf Futterleguminosen aus unterschiedlichen Nutzungssystemen

Institute of Animal Nutrition, Livestock Products and Nutrition Physiology (TTE), Department of Agrobiotechnology, IFA-Tulln, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 11, 1190 Wien,

Grass and Forage Science/Organic Agriculture Group, Institute of Crop Science and Plant Breeding, University of Kiel, Hermann-Rodewald Str. 9, 24118 Kiel,

Grassland Management and Cultural Landscape, Institute for Plant Production and Cultural Landscape, Agricultural Research and Education Center Raumberg-Gumpenstein, Raumberg 38, 8952 Irdning,

<trans-abstract xml:lang="de" xmlns="http://www.w3.org/1999/xhtml"><p>Zwei <italic>in vitro</italic> Methoden wurden dahingehend überprüft, das Potential des Gehaltes an umsetzbarer Energie (ME) von Futterleguminosen zu schätzen: die Tilley und Terry- (TT) und die Pepsin-Cellulase-Methode (CM). Unterschiedliche Proben aus Weißklee, Rotklee, Kuraklee, Luzerne und Hornklee wurden von Feldversuchen gewonnen, welche verschiedene Nutzungssysteme darstellen. Die CM-Methode war präziser durch die hohe Wiederholbarkeit zwischen und innerhalb der Analysenchargen, sowie für die Standardproben mit bekannter <italic>in vivo</italic> Verdaulichkeit. ME-Gehalte waren durchwegs höher wenn mit der CM-Methode ermittelt, mit einer Differenz von bis zu 1,5 MJ ME/kg TS im Vergleich zur TT-Methode. Auch wenn Weißklee die Spezies mit dem höchsten ME-Gehalt war, der Einfluss der Leguminosenart über alle Aufwüchse und Nutzungssysteme hinweg war nicht konsistent. Solche Beobachtungen beeinflussen die Wahl der Methode für die Ermittlung des ME-Gehaltes bei großen Datenmengen.</p></trans-abstract>

Zwei in vitro Methoden wurden dahingehend überprüft, das Potential des Gehaltes an umsetzbarer Energie (ME) von Futterleguminosen zu schätzen: die Tilley und Terry- (TT) und die Pepsin-Cellulase-Methode (CM). Unterschiedliche Proben aus Weißklee, Rotklee, Kuraklee, Luzerne und Hornklee wurden von Feldversuchen gewonnen, welche verschiedene Nutzungssysteme darstellen. Die CM-Methode war präziser durch die hohe Wiederholbarkeit zwischen und innerhalb der Analysenchargen, sowie für die Standardproben mit bekannter in vivo Verdaulichkeit. ME-Gehalte waren durchwegs höher wenn mit der CM-Methode ermittelt, mit einer Differenz von bis zu 1,5 MJ ME/kg TS im Vergleich zur TT-Methode. Auch wenn Weißklee die Spezies mit dem höchsten ME-Gehalt war, der Einfluss der Leguminosenart über alle Aufwüchse und Nutzungssysteme hinweg war nicht konsistent. Solche Beobachtungen beeinflussen die Wahl der Methode für die Ermittlung des ME-Gehaltes bei großen Datenmengen.

Die Bodenkultur: Journal of Land Management, Food and Environment

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Using  methods to estimate metabolizable energy content of five forage legumes harvested under different defoliation systems / Einsatz von  Methoden zur Schätzung der umsetzbaren Energie in fünf Futterleguminosen aus unterschiedlichen Nutzungssystemen

Two in vitro methods were tested to establish their potential to predict the metabolizable energy (ME) content of forage legumes: the Tilley and Terry (TT)...

Using in vitro methods to estimate metabolizable energy content of five forage legumes harvested under different defoliation systems / Einsatz von in vitro Methoden zur Schätzung der umsetzbaren Energie in fünf Futterleguminosen aus unterschiedlichen Nutzungssystemen

Article Category: Research Article

Published Online: Dec 02, 2016

Page range: 121 - 131

Received: Apr 22, 2016

Accepted: Jul 20, 2016

DOI: https://doi.org/10.1515/boku-2016-0011

Keywords
Forage legumes, metabolizable energy, NIRS, Tilley and Terry method, pepsin-cellulase method

© 2016 De Gruyter, www.degruyter.com/view/j/boku

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Using in vitro methods to estimate metabolizable energy content of five forage legumes harvested under different defoliation systems / Einsatz von in vitro Methoden zur Schätzung der umsetzbaren Energie in fünf Futterleguminosen aus unterschiedlichen Nutzungssystemen

Article Category: Research Article

Published Online: Dec 02, 2016

Page range: 121 - 131

Received: Apr 22, 2016

Accepted: Jul 20, 2016

DOI: https://doi.org/10.1515/boku-2016-0011

KeywordsForage legumes, metabolizable energy, NIRS, Tilley and Terry method, pepsin-cellulase method

© 2016 De Gruyter, www.degruyter.com/view/j/boku

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords
Forage legumes, metabolizable energy, NIRS, Tilley and Terry method, pepsin-cellulase method