<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Microarray data of non-functioning pituitary adenomas (NFPAs) were analyzed to disclose novel genes and pathways involved in NFPA tumorigenesis. Raw microarray data were downloaded from Gene Expression Omnibus. Data pre-treatment and differential analysis were conducted using packages in <italic>R</italic>. Functional and pathway enrichment analyses were performed using package GOs-tats. A protein-protein interaction (PPI) network was constructed using server STRING and Cytoscape. Known genes involved in pituitary adenomas (PAs), were obtained from the Comparative Toxicogenomics Database. A total of 604 differentially expressed genes (DEGs) were identifed between NFPAs and controls, including 177 up- and 427 down-regulated genes. Jak-STAT and p53 signaling pathways were significantly enriched by DEGs. The PPI network of DEGs was constructed, containing 99 up- and 288 down-regulated known disease genes (<italic>e.g. EGFR</italic> and <italic>ESR1</italic>) as well as 16 up- and 17 down-regulated potential novel NFPAs-related genes (<italic>e.g. COL4A5</italic>, <italic>LHX3</italic>, <italic>MSN</italic>, and <italic>GHSR</italic>). Genes like <italic>COL4A5</italic>, <italic>LHX3</italic>, <italic>MSN</italic>, and <italic>GHSR</italic> and pathways such as p53 signaling and Jak-STAT signaling, might participate in NFPA development. Although further validations are required, these findings might provide guidance for future basic and therapy researches.</p></abstract>

Microarray data of non-functioning pituitary adenomas (NFPAs) were analyzed to disclose novel genes and pathways involved in NFPA tumorigenesis. Raw microarray data were downloaded from Gene Expression Omnibus. Data pre-treatment and differential analysis were conducted using packages in R. Functional and pathway enrichment analyses were performed using package GOs-tats. A protein-protein interaction (PPI) network was constructed using server STRING and Cytoscape. Known genes involved in pituitary adenomas (PAs), were obtained from the Comparative Toxicogenomics Database. A total of 604 differentially expressed genes (DEGs) were identifed between NFPAs and controls, including 177 up- and 427 down-regulated genes. Jak-STAT and p53 signaling pathways were significantly enriched by DEGs. The PPI network of DEGs was constructed, containing 99 up- and 288 down-regulated known disease genes (e.g. EGFR and ESR1) as well as 16 up- and 17 down-regulated potential novel NFPAs-related genes (e.g. COL4A5, LHX3, MSN, and GHSR). Genes like COL4A5, LHX3, MSN, and GHSR and pathways such as p53 signaling and Jak-STAT signaling, might participate in NFPA development. Although further validations are required, these findings might provide guidance for future basic and therapy researches.

Microarray technology reveals potentially novel genes and pathways involved in non-functioning pituitary adenomas

Balkan Journal of Medical Genetics

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Microarray data of non-functioning pituitary adenomas (NFPAs) were analyzed to disclose novel genes and pathways involved in NFPA tumorigenesis. Raw microarray...

Microarray technology reveals potentially novel genes and pathways involved in non-functioning pituitary adenomas

Article Category: Original Article

Published Online: Dec 31, 2016

Page range: 5 - 16

DOI: https://doi.org/10.1515/bjmg-2016-0030

Keywords
Differentially expressed genes (DEGs), Functional enrichment analysis, Microarray, Non-functioning pituitary adenomas (NFPAs), Protein-protein interaction (PPI)

© 2016 Qiao X, Wang H, Wang X, Zhao B, Liu J

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Microarray technology reveals potentially novel genes and pathways involved in non-functioning pituitary adenomas

Article Category: Original Article

Published Online: Dec 31, 2016

Page range: 5 - 16

DOI: https://doi.org/10.1515/bjmg-2016-0030

KeywordsDifferentially expressed genes (DEGs), Functional enrichment analysis, Microarray, Non-functioning pituitary adenomas (NFPAs), Protein-protein interaction (PPI)

© 2016 Qiao X, Wang H, Wang X, Zhao B, Liu J

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords
Differentially expressed genes (DEGs), Functional enrichment analysis, Microarray, Non-functioning pituitary adenomas (NFPAs), Protein-protein interaction (PPI)