<abstract xmlns="http://www.w3.org/1999/xhtml">For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguish <italic>Pseudomonas</italic> and <italic>Stenotrophomonas</italic> genera, considering acquired resistance of <italic>Pseudomonas</italic> species, as well as the intrinsic resistance of <italic>Stenotrophomonas</italic> species. This is why in the identification of the 51 isolates originated from fish, the following methods were used: standard PCR, 16S rRNA gene sequencing, and MALDI-TOF.The results of the standard PCR test, 16S rRNA gene sequencing and MALDI-TOF analysis confirmed 35 strains to belong to the <italic>Pseudomonas</italic> genus. Standard PCR test and VITEK MS device confirmed that 10 strains belong to <italic>Stenotrophomonas maltophilia</italic> species.Three strains were positive in both standard PCR tests for <italic>Pseudomonas</italic> and <italic>Stenotrpohomonas</italic>. 16S rRNA gene sequencing identified these 3 strains to be 99% <italic>Pseudomonas</italic> sp. and 99% <italic>Stenotrophomonas</italic> sp. VITEK MS first identified these three strains as 99% <italic>Stenotrophomonas</italic>, and in the repeated identification it identified them as 99% <italic>Pseudomonas</italic>. MALDI TOF/TOF 4800 Plus device identified these strains as <italic>Stenotrophomonas</italic>.Three strains were negative in both standard PCR tests for <italic>Pseudomonas</italic> and <italic>Stenotrpohomonas</italic>. 16S rRNA gene sequencing identified these 3 strains to be 99% <italic>Pseudomonas</italic> sp. and 99% <italic>Stenotrophomonas</italic> sp. VITEK MS first identified these three strains as 99% <italic>Stenotrophomonas</italic>, and in the repeated identification it identified them as 99% <italic>Pseudomonas</italic>. MALDI TOF/TOF 4800 Plus device identified these strains as <italic>Stenotrophomonas</italic>.Although modern test methods that have very high specificity (PCR, 16S rRNA gene sequencing, MALDI TOF) were used in this study, precise differentiation between <italic>Pseudomonas</italic> and <italic>Stenotrophomonas</italic> species for 6 isolates could not be reached using the above mentioned methods.</abstract>

For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguish Pseudomonas and Stenotrophomonas genera, considering acquired resistance of Pseudomonas species, as well as the intrinsic resistance of Stenotrophomonas species. This is why in the identification of the 51 isolates originated from fish, the following methods were used: standard PCR, 16S rRNA gene sequencing, and MALDI-TOF.The results of the standard PCR test, 16S rRNA gene sequencing and MALDI-TOF analysis confirmed 35 strains to belong to the Pseudomonas genus. Standard PCR test and VITEK MS device confirmed that 10 strains belong to Stenotrophomonas maltophilia species.Three strains were positive in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas.Three strains were negative in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas.Although modern test methods that have very high specificity (PCR, 16S rRNA gene sequencing, MALDI TOF) were used in this study, precise differentiation between Pseudomonas and Stenotrophomonas species for 6 isolates could not be reached using the above mentioned methods.

Differentiation between <italic>Pseudomonas</italic> and <italic>Stenotrophomonas</italic> Species Isolated from Fish Using Molecular and MALDI-TOF Method

Department of Microbiology, Faculty of Veterinary Medicine University of Belgrade, Belgrade,

Innovation Center of Faculty of Technology and Metallurgy, University of Belgrade, Belgrade,

Laboratory for Microbial Molecular Genetics and Ecology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade,

for Human Ecology and Ecotoxicology, Institute of Public Health Belgrade, Belgrade,

Central Laboratory, Biocentre Ltd., Zagreb,

Laboratory for System Biomedicine and Centre for Proteomics and mass spectrometry, “RuđerBošković” Institute, Zagreb,

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Differentiation between  and  Species Isolated from Fish Using Molecular and MALDI-TOF Method

For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguish Pseudomonas and Stenotrophomonas genera, considering...

Differentiation between Pseudomonas and Stenotrophomonas Species Isolated from Fish Using Molecular and MALDI-TOF Method

Published Online: Sep 29, 2016

Page range: 304 - 316

Received: Apr 28, 2016

Accepted: Jun 29, 2016

DOI: https://doi.org/10.1515/acve-2016-0027

Keywords
fish, PCR, 16S rRNA, MALDI-TOF

© 2016 Aksentijević Ksenija et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Differentiation between Pseudomonas and Stenotrophomonas Species Isolated from Fish Using Molecular and MALDI-TOF Method

Published Online: Sep 29, 2016

Page range: 304 - 316

Received: Apr 28, 2016

Accepted: Jun 29, 2016

DOI: https://doi.org/10.1515/acve-2016-0027

Keywordsfish, PCR, 16S rRNA, MALDI-TOF

© 2016 Aksentijević Ksenija et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
fish, PCR, 16S rRNA, MALDI-TOF