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Changes in sperm quality and testicular structure in a rat model of type 1 diabetes

Sureeporn Nak-ung
Sureeporn Nak-ung
, 
Nutnicha Nakprom
Nutnicha Nakprom
, 
Chonikan Maneengam
Chonikan Maneengam
, 
Sutisa Nudmamud-Thanoi
Sutisa Nudmamud-Thanoi
 and 
Samur Thanoi
Samur Thanoi
   | Sep 25, 2019
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Article Category: Original article
Published Online: Sep 25, 2019
Page range: 141 - 147
DOI: https://doi.org/10.1515/abm-2019-0014
Keywords
© 2018 Sureeporn Nak-ung et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

Sperm quality parameters in rats in the group with streptozotocin-induced diabetes mellitus (gray bars) compared with those in vehicle treated rats in the control group (unfilled bars). (A) Percentage of sperm motility. (B) Percentage of normal sperm morphology. (C) Sperm concentration. Bars represent mean percentages or concentrations, and error bars represent standard error of the mean; t test ***P< 0.001
Sperm quality parameters in rats in the group with streptozotocin-induced diabetes mellitus (gray bars) compared with those in vehicle treated rats in the control group (unfilled bars). (A) Percentage of sperm motility. (B) Percentage of normal sperm morphology. (C) Sperm concentration. Bars represent mean percentages or concentrations, and error bars represent standard error of the mean; t test ***P< 0.001

Figure 2

The diameter of seminiferous tubules in rats with streptozotocin-induced diabetes mellitus (gray bars) compared with that in vehicle treated rats in the control group (unfilled bars). Bars represent mean diameters, and error bars represent standard error of the mean; t test *P< 0.05
The diameter of seminiferous tubules in rats with streptozotocin-induced diabetes mellitus (gray bars) compared with that in vehicle treated rats in the control group (unfilled bars). Bars represent mean diameters, and error bars represent standard error of the mean; t test *P< 0.05

Figure 3

Seminiferous tubule morphology. (A) Normal morphology in vehicle-treated rats. Changes in morphology in rats with streptozotocin- induced diabetes mellitus (B) separation of the germinal epithelium (arrows), (C) vacuolization (arrows), (D) luminal sloughing of germ cells (arrows), (E) irregular tube (dashed circle), and (F) atrophy (dashed circle). Scale bars represent 50 μm (magnification 200×)
Seminiferous tubule morphology. (A) Normal morphology in vehicle-treated rats. Changes in morphology in rats with streptozotocin- induced diabetes mellitus (B) separation of the germinal epithelium (arrows), (C) vacuolization (arrows), (D) luminal sloughing of germ cells (arrows), (E) irregular tube (dashed circle), and (F) atrophy (dashed circle). Scale bars represent 50 μm (magnification 200×)

Figure 4

Morphological changes of seminiferous tubules in rats with streptozotocin-induced diabetes mellitus (gray bars) compared with those in vehicle-treated rats in the control group (unfilled bars). Bars represent mean percentages of abnormalities, and error bars represent standard error of the mean. t test #P< 0.1; *P< 0.05; **P< 0.01; ***P< 0.001
Morphological changes of seminiferous tubules in rats with streptozotocin-induced diabetes mellitus (gray bars) compared with those in vehicle-treated rats in the control group (unfilled bars). Bars represent mean percentages of abnormalities, and error bars represent standard error of the mean. t test #P< 0.1; *P< 0.05; **P< 0.01; ***P< 0.001

Figure 5

Proportion of seminiferous tubules at various stages of development in rats with streptozotocin-induced diabetes mellitus (gray bars) compared with that in vehicle-treated rats in the control group (unfilled bars). Bars represent mean percentages at the various stages, and error bars represent standard error of the mean. t test ***P< 0.001
Proportion of seminiferous tubules at various stages of development in rats with streptozotocin-induced diabetes mellitus (gray bars) compared with that in vehicle-treated rats in the control group (unfilled bars). Bars represent mean percentages at the various stages, and error bars represent standard error of the mean. t test ***P< 0.001

Body weight, testicular weight, and the testicular weight percentage of body weight in rats in rats in the group with streptozotocin-induced diabetes mellitus and in the vehicle treated control group

Control groupDiabetic groupP
(n = 8)(n = 7)
Body weight (g)453 ± 5.6268 ± 18.8***<0.001
Testis weight (g)2.1 ± 0.011.8 ± 0.10*0.02
Testicular %body weight weight as0.45 ± 0.010.68 ± 0.05**0.003
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