<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_abm-2019-0014_s_004_w2aab3b7b1b1b6b1aab1c16b1Aa"><h3>Background</h3><p>Chronic hyperglycemia is a characteristic of diabetes mellitus (DM). Long-lasting hyperglycemia can generate oxidative stress and reactive oxygen species. The effect of this condition on sperm quality and spermatogenesis leads to male infertility and reproductive dysfunction.</p></sec><sec id="j_abm-2019-0014_s_005_w2aab3b7b1b1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To investigate changes in sperm quality, morphology of testicular structure, and stage of development of seminiferous tubules in a streptozotocin (STZ)-induced rat model of type 1 DM.</p></sec><sec id="j_abm-2019-0014_s_006_w2aab3b7b1b1b6b1aab1c16b3Aa"><h3>Methods</h3><p>We divided 15 male Sprague Dawley rats into 2 groups. DM was induced in 7 rats using STZ (60 mg/kg intraperitoneally), while the other 8 were treated with citrate buffer as a vehicle control group. Rat semen was collected for quality measurements including motility, normal morphology, and concentration. Morphological changes in testicular structure and stage of development of seminiferous tubules were investigated by histology with hematoxylin and eosin (HE) staining.</p></sec><sec id="j_abm-2019-0014_s_007_w2aab3b7b1b1b6b1aab1c16b4Aa"><h3>Results</h3><p>Significant decreases in all parameters of sperm quality and testicular weight were found in rats with induced DM. Moreover, abnormal morphology of seminiferous tubules including separation of the germinal epithelium, vacuolization, luminal sloughing of germ cells, and tubular atrophy was increased significantly in these rats, while the proportion of their seminiferous tubules at an early stage of development was significantly higher, but was dramatically decreased in the late stage of development when compared with that in vehicle-treated control rats.</p></sec><sec id="j_abm-2019-0014_s_008_w2aab3b7b1b1b6b1aab1c16b5Aa"><h3>Conclusions</h3><p>DM has adverse effects on sperm quality, testicular structure, and development of seminiferous tubules. These findings may reflect the male infertility and reproductive dysfunction seen in patients with type 1 DM.</p></sec></abstract>

BackgroundChronic hyperglycemia is a characteristic of diabetes mellitus (DM). Long-lasting hyperglycemia can generate oxidative stress and reactive oxygen species. The effect of this condition on sperm quality and spermatogenesis leads to male infertility and reproductive dysfunction.ObjectivesTo investigate changes in sperm quality, morphology of testicular structure, and stage of development of seminiferous tubules in a streptozotocin (STZ)-induced rat model of type 1 DM.MethodsWe divided 15 male Sprague Dawley rats into 2 groups. DM was induced in 7 rats using STZ (60 mg/kg intraperitoneally), while the other 8 were treated with citrate buffer as a vehicle control group. Rat semen was collected for quality measurements including motility, normal morphology, and concentration. Morphological changes in testicular structure and stage of development of seminiferous tubules were investigated by histology with hematoxylin and eosin (HE) staining.ResultsSignificant decreases in all parameters of sperm quality and testicular weight were found in rats with induced DM. Moreover, abnormal morphology of seminiferous tubules including separation of the germinal epithelium, vacuolization, luminal sloughing of germ cells, and tubular atrophy was increased significantly in these rats, while the proportion of their seminiferous tubules at an early stage of development was significantly higher, but was dramatically decreased in the late stage of development when compared with that in vehicle-treated control rats.ConclusionsDM has adverse effects on sperm quality, testicular structure, and development of seminiferous tubules. These findings may reflect the male infertility and reproductive dysfunction seen in patients with type 1 DM.

Changes in sperm quality and testicular structure in a rat model of type 1 diabetes

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

BackgroundChronic hyperglycemia is a characteristic of diabetes mellitus (DM). Long-lasting hyperglycemia can generate oxidative stress and reactive oxygen...

Changes in sperm quality and testicular structure in a rat model of type 1 diabetes

Article Category: Original article

Published Online: Sep 25, 2019

Page range: 141 - 147

DOI: https://doi.org/10.1515/abm-2019-0014

Keywords
diabetes mellitus type 1, infertility, male, reactive oxygen species, spermatogenesis, spermatozoa, sperm maturation, teratozoospermia, testis

© 2018 Sureeporn Nak-ung et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Changes in sperm quality and testicular structure in a rat model of type 1 diabetes

Article Category: Original article

Published Online: Sep 25, 2019

Page range: 141 - 147

DOI: https://doi.org/10.1515/abm-2019-0014

Keywordsdiabetes mellitus type 1, infertility, male, reactive oxygen species, spermatogenesis, spermatozoa, sperm maturation, teratozoospermia, testis

© 2018 Sureeporn Nak-ung et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
diabetes mellitus type 1, infertility, male, reactive oxygen species, spermatogenesis, spermatozoa, sperm maturation, teratozoospermia, testis