<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.1004.494_s_001_w2aab3b7b2b1b6b1aab1c16b1Aa"><h3>Background</h3><p>Immunotherapy, during which the immune system of the patient is manipulated to act against tumors has been among the most successful methods in the treatment of breast cancer, a leading cause of mortality among women worldwide.</p></sec><sec id="j_1905-7415.1004.494_s_002_w2aab3b7b2b1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To investigate the immunotherapeutic efficacy of <italic>Lactobacillus casei</italic> lysate as an adjuvant in combination with a heated-4T1 mammary carcinoma cell lysate in a model of breast cancer.</p></sec><sec id="j_1905-7415.1004.494_s_003_w2aab3b7b2b1b6b1aab1c16b3Aa"><h3>Methods</h3><p>After ethics committee approval of all animal procedures, a murine model of breast cancer was induced in BALB/c mice using 4T1 cells. These mice were immunized with a combination of lysates of heated 4T1 cells and <italic>L. casei</italic>. Subsequent changes in tumor size and weight, and the production of TNF-α, IL-2, IL-12, IL-17, and IL13 were measured. Lung weights were measured as an indicator of metastasis to other organs.</p></sec><sec id="j_1905-7415.1004.494_s_004_w2aab3b7b2b1b6b1aab1c16b4Aa"><h3>Results</h3><p>The tumor size and weight in mice immunized with the combined vaccine were significantly reduced compared with controls. The combined immunotherapy altered the pattern of cytokine production to the advantage of antitumor immunity, and was significantly more potent than immunization with heated-4T1-cell lysate or <italic>L. casei</italic> lysate alone.</p></sec><sec id="j_1905-7415.1004.494_s_005_w2aab3b7b2b1b6b1aab1c16b5Aa"><h3>Conclusions</h3><p>Coadministration of <italic>L. casei</italic> lysate enhanced the immunotherapeutic efficacy of the heated-4T1-cell lysate as a source of tumor-associated antigens. <italic>L. casei</italic> can potentially be used as an adjuvant combined with sources of tumor antigens in the treatment of cancers, and as a safe alternative to the current adjuvants that cause greater irritation to hosts. Further studies are required to clarify the mechanisms underlying these effects.</p></sec></abstract>

BackgroundImmunotherapy, during which the immune system of the patient is manipulated to act against tumors has been among the most successful methods in the treatment of breast cancer, a leading cause of mortality among women worldwide.ObjectivesTo investigate the immunotherapeutic efficacy of Lactobacillus casei lysate as an adjuvant in combination with a heated-4T1 mammary carcinoma cell lysate in a model of breast cancer.MethodsAfter ethics committee approval of all animal procedures, a murine model of breast cancer was induced in BALB/c mice using 4T1 cells. These mice were immunized with a combination of lysates of heated 4T1 cells and L. casei. Subsequent changes in tumor size and weight, and the production of TNF-α, IL-2, IL-12, IL-17, and IL13 were measured. Lung weights were measured as an indicator of metastasis to other organs.ResultsThe tumor size and weight in mice immunized with the combined vaccine were significantly reduced compared with controls. The combined immunotherapy altered the pattern of cytokine production to the advantage of antitumor immunity, and was significantly more potent than immunization with heated-4T1-cell lysate or L. casei lysate alone.ConclusionsCoadministration of L. casei lysate enhanced the immunotherapeutic efficacy of the heated-4T1-cell lysate as a source of tumor-associated antigens. L. casei can potentially be used as an adjuvant combined with sources of tumor antigens in the treatment of cancers, and as a safe alternative to the current adjuvants that cause greater irritation to hosts. Further studies are required to clarify the mechanisms underlying these effects.

Immunotherapeutic efficacy of a <italic>Lactobacillus casei</italic> lysate as an adjuvant combined with a heated-4T1 mammary carcinoma cell lysate in a murine model of breast cancer

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Immunotherapeutic efficacy of a  lysate as an adjuvant combined with a heated-4T1 mammary carcinoma cell lysate in a murine model of breast cancer

BackgroundImmunotherapy, during which the immune system of the patient is manipulated to act against tumors has been among the most successful methods in the...

Immunotherapeutic efficacy of a Lactobacillus casei lysate as an adjuvant combined with a heated-4T1 mammary carcinoma cell lysate in a murine model of breast cancer

Article Category: Original article

Published Online: Jan 31, 2017

Page range: 327 - 334

DOI: https://doi.org/10.5372/1905-7415.1004.494

Keywords
Cancer immunotherapy, HSP, TNF-α, tumor, vaccine

© 2016 Ruhollah Dorostkar, Mohammad Sadegh Hashemzadeh, Sajjad Jafari, Mahdi Tat, Majdedin Ghalavand, Mohammad Hossein Asghari, Milad Moloudizargari

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Immunotherapeutic efficacy of a Lactobacillus casei lysate as an adjuvant combined with a heated-4T1 mammary carcinoma cell lysate in a murine model of breast cancer

Article Category: Original article

Published Online: Jan 31, 2017

Page range: 327 - 334

DOI: https://doi.org/10.5372/1905-7415.1004.494

KeywordsCancer immunotherapy, HSP, TNF-α, tumor, vaccine

© 2016 Ruhollah Dorostkar, Mohammad Sadegh Hashemzadeh, Sajjad Jafari, Mahdi Tat, Majdedin Ghalavand, Mohammad Hossein Asghari, Milad Moloudizargari

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Cancer immunotherapy, HSP, TNF-α, tumor, vaccine