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Protective/restorative role of the adipose tissue-derived mesenchymal stem cells on the radioiodine-induced salivary gland damage in rats

Güleser Saylam
Güleser Saylam
, 
Ömer Bayır
Ömer Bayır
, 
Salih Sinan Gültekin
Salih Sinan Gültekin
, 
Ferda Alparslan Pınarlı
Ferda Alparslan Pınarlı
, 
Ünsal Han
Ünsal Han
, 
Mehmet Hakan Korkmaz
Mehmet Hakan Korkmaz
, 
Mehmet Eser Sancaktar
Mehmet Eser Sancaktar
, 
İlkan Tatar
İlkan Tatar
, 
Mustafa Fevzi Sargon
Mustafa Fevzi Sargon
 and 
Emel Çadallı Tatar
Emel Çadallı Tatar
   | Jun 14, 2017
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Article Category: Research Article
Published Online: Jun 14, 2017
Page range: 307 - 316
Received: Dec 21, 2016
Accepted: Apr 07, 2017
DOI: https://doi.org/10.1515/raon-2017-0022
© 2017 Güleser Saylam, Ömer Bayır, Salih Sinan Gültekin, Ferda Alparslan Pınarlı, Ünsal Han, Mehmet Hakan Korkmaz, Mehmet Eser Sancaktar, İlkan Tatar, Mustafa Fevzi Sargon, Emel Çadallı Tatar
This work is licensed under the Creative Commons Attribution 4.0 International License.

Background

To analyze protective/regenerative effects of adipose tissue-derived mesenchymal stem cells (ADMSC) on 131I-Radioiodine (RAI)-induced salivary gland damage in rats.

Materials and Methods

Study population consisted of controls (n:6) and study groups (n:54): RAI (Group 1), ADMSC (Group 2), amifostine (Group 3), RAI+amifostine (Group 4), concomitant RAI+ADMSC (Group 5) and RAI+ADMSC after 48 h (Group 6). We used light microscopy (LM), transmission electron microscopy (TEM), and salivary gland scintigraphy (SGS), and analyzed data statistically.

Results

We observed the homing of ADMSC in salivary glands at 1st month on LM. RAI exposure affected necrosis, periductal fibrosis, periductal sclerosis, vascular sclerosis and the total sum score were in a statistically significant manner (P < 0.05). Intragroup comparisons with LM at 1st and 6th months revealed statistically significant improvements in Group 6 (P < 0.05) but not in Groups 4 and 5. Intergroup comparisons of the total score showed that Groups 4 and 5 in 1st month and Group 6 in 6th month had the lowest values. TEM showed vacuolization, edema, and fibrosis at 1st month, and an improvement in damage in 6th month in Groups 5 and 6. SGSs revealed significant differences for the maximum secretion ratio (Smax) (P = 0.01) and the gland-to-background ratio at a maximum count (G/BGmax) (P = 0. 01) at 1st month, for G/BGmax (P = 0.01), Smax (P = 0.01) and the time to reach the maximum count ratio over the time to reach the minimum count (Tmax/Tmin) (P = 0.03) at 6th month. 1st and 6th month scans showed differences for Smax and G/BGmax (P = 0.04), but not for Tmax/Tmin (p > 0.05). We observed a significant deterioration in gland function in group 1, whereas, mild to moderate deteriorations were seen in protective treatment groups.

Conclusions

Our results indicated that ADMSC might play a promising role as a protective/regenerative agent against RAI-induced salivary gland dysfunction.
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Language:
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