<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_raon-2017-0022_s_023">
<h3>Background</h3>
<p>To analyze protective/regenerative effects of adipose tissue-derived mesenchymal stem cells (ADMSC) on <sup>131</sup>I-Radioiodine (RAI)-induced salivary gland damage in rats.</p>
</sec>
<sec id="j_raon-2017-0022_s_024">
<h3>Materials and Methods</h3>
<p>Study population consisted of controls (n:6) and study groups (n:54): RAI (Group 1), ADMSC (Group 2), amifostine (Group 3), RAI+amifostine (Group 4), concomitant RAI+ADMSC (Group 5) and RAI+ADMSC after 48 h (Group 6). We used light microscopy (LM), transmission electron microscopy (TEM), and salivary gland scintigraphy (SGS), and analyzed data statistically.</p>
</sec>
<sec id="j_raon-2017-0022_s_025">
<h3>Results</h3>
<p>We observed the homing of ADMSC in salivary glands at 1<sup>st</sup> month on LM. RAI exposure affected necrosis, periductal fibrosis, periductal sclerosis, vascular sclerosis and the total sum score were in a statistically significant manner (<italic>P</italic> &lt; 0.05). Intragroup comparisons with LM at 1<sup>st</sup> and 6<sup>th</sup> months revealed statistically significant improvements in Group 6 (<italic>P</italic> &lt; 0.05) but not in Groups 4 and 5. Intergroup comparisons of the total score showed that Groups 4 and 5 in 1<sup>st</sup> month and Group 6 in 6<sup>th</sup> month had the lowest values. TEM showed vacuolization, edema, and fibrosis at 1<sup>st</sup> month, and an improvement in damage in 6<sup>th</sup> month in Groups 5 and 6. SGSs revealed significant differences for the maximum secretion ratio (Smax) (<italic>P</italic> = 0.01) and the gland-to-background ratio at a maximum count (G/BGmax) (<italic>P</italic> = 0. 01) at 1<sup>st</sup> month, for G/BGmax (<italic>P</italic> = 0.01), Smax (<italic>P</italic> = 0.01) and the time to reach the maximum count ratio over the time to reach the minimum count (Tmax/Tmin) (<italic>P</italic> = 0.03) at 6<sup>th</sup> month. 1<sup>st</sup> and 6<sup>th</sup> month scans showed differences for Smax and G/BGmax (<italic>P</italic> = 0.04), but not for Tmax/Tmin (<italic>p</italic> > 0.05). We observed a significant deterioration in gland function in group 1, whereas, mild to moderate deteriorations were seen in protective treatment groups.</p>
</sec>
<sec id="j_raon-2017-0022_s_026">
<h3>Conclusions</h3>
<p>Our results indicated that ADMSC might play a promising role as a protective/regenerative agent against RAI-induced salivary gland dysfunction.</p>
</sec>
</abstract>

Background
To analyze protective/regenerative effects of adipose tissue-derived mesenchymal stem cells (ADMSC) on 131I-Radioiodine (RAI)-induced salivary gland damage in rats.


Materials and Methods
Study population consisted of controls (n:6) and study groups (n:54): RAI (Group 1), ADMSC (Group 2), amifostine (Group 3), RAI+amifostine (Group 4), concomitant RAI+ADMSC (Group 5) and RAI+ADMSC after 48 h (Group 6). We used light microscopy (LM), transmission electron microscopy (TEM), and salivary gland scintigraphy (SGS), and analyzed data statistically.


Results
We observed the homing of ADMSC in salivary glands at 1st month on LM. RAI exposure affected necrosis, periductal fibrosis, periductal sclerosis, vascular sclerosis and the total sum score were in a statistically significant manner (P < 0.05). Intragroup comparisons with LM at 1st and 6th months revealed statistically significant improvements in Group 6 (P < 0.05) but not in Groups 4 and 5. Intergroup comparisons of the total score showed that Groups 4 and 5 in 1st month and Group 6 in 6th month had the lowest values. TEM showed vacuolization, edema, and fibrosis at 1st month, and an improvement in damage in 6th month in Groups 5 and 6. SGSs revealed significant differences for the maximum secretion ratio (Smax) (P = 0.01) and the gland-to-background ratio at a maximum count (G/BGmax) (P = 0. 01) at 1st month, for G/BGmax (P = 0.01), Smax (P = 0.01) and the time to reach the maximum count ratio over the time to reach the minimum count (Tmax/Tmin) (P = 0.03) at 6th month. 1st and 6th month scans showed differences for Smax and G/BGmax (P = 0.04), but not for Tmax/Tmin (p > 0.05). We observed a significant deterioration in gland function in group 1, whereas, mild to moderate deteriorations were seen in protective treatment groups.


Conclusions
Our results indicated that ADMSC might play a promising role as a protective/regenerative agent against RAI-induced salivary gland dysfunction.

Protective/restorative role of the adipose tissue-derived mesenchymal stem cells on the radioiodine-induced salivary gland damage in rats

University of Health Sciences, Dışkapı Yıldırım Beyazıt Training and Research Hospital

Department of Stem Cell and Genetic Diagnostic Center

Yıldırım Beyazıt University, Faculty of Medicine

Ministry of Health, Samsun Training and Reseach Hospital

Hacettepe University, Faculty of Medicine

Radiology and Oncology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Background
To analyze protective/regenerative effects of adipose tissue-derived mesenchymal stem cells (ADMSC) on 131I-Radioiodine (RAI)-induced salivary gland damage in rats.


Materials and Methods
Study population consisted of controls (n:6) and study groups (n:54): RAI (Group 1), ADMSC (Group 2), amifostine (Group 3), RAI+amifostine (Group 4), concomitant RAI+ADMSC (Group 5) and RAI+ADMSC after 48 h (Group 6). We used light microscopy (LM), transmission electron microscopy (TEM), and salivary gland scintigraphy (SGS), and analyzed data statistically.


Results
We observed the homing of ADMSC in salivary glands at 1st month on LM. RAI exposure affected necrosis, periductal fibrosis, periductal sclerosis, vascular sclerosis and the total sum score were in a statistically significant manner (P  0.05). We observed a significant deterioration in gland function in group 1, whereas, mild to moderate deteriorations were seen in protective treatment groups.


Conclusions
Our results indicated that ADMSC might play a promising role as a protective/regenerative agent against RAI-induced salivary gland dysfunction.

Background
To analyze protective/regenerative effects of adipose tissue-derived mesenchymal stem cells (ADMSC) on 131I-Radioiodine (RAI)-induced salivary gland...

Protective/restorative role of the adipose tissue-derived mesenchymal stem cells on the radioiodine-induced salivary gland damage in rats

Article Category: Research Article

Published Online: Jun 14, 2017

Page range: 307 - 316

Received: Dec 21, 2016

Accepted: Apr 07, 2017

DOI: https://doi.org/10.1515/raon-2017-0022

© 2017 Güleser Saylam, Ömer Bayır, Salih Sinan Gültekin, Ferda Alparslan Pınarlı, Ünsal Han, Mehmet Hakan Korkmaz, Mehmet Eser Sancaktar, İlkan Tatar, Mustafa Fevzi Sargon, Emel Çadallı Tatar

This work is licensed under the Creative Commons Attribution 4.0 International License.