Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies
Published Online: Sep 13, 2019
Page range: 391 - 398
Received: Aug 15, 2018
Accepted: Aug 07, 2019
DOI: https://doi.org/10.2478/jvetres-2019-0057
Keywords
© 2019 M. Gehrke et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
A comprehensive description is presented of four novel cases of
Material and Methods
Four amorphic foetuses were delivered by three cows. Tissue samples were collected during autopsy, embedded in paraffin, sectioned, and stained with haematoxylin and eosin. Genomic DNA was isolated from tissue samples of abnormal foetuses and from blood leukocytes of their healthy siblings. PCR reactions were used to reveal the presence of Y-linked genes (SRY and AMELY) and an X-linked gene (AMELX).
Results
All foetuses were classified to the group
Conclusion
Foetuses had monozygotic origin. Histological analysis showed a low level of development of tissues of meso- and ectodermal origin, as well as features of degrading patterns.