<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2019-0057_s_005">
<h3>Introduction</h3>
<p>A comprehensive description is presented of four novel cases of<italic>amorphus globosus (ag)</italic> foetuses originating from multiple pregnancies of Polish Holstein cows.</p></sec>
<sec id="j_jvetres-2019-0057_s_006">
<h3>Material and Methods</h3>
<p>Four amorphic foetuses were delivered by three cows. Tissue samples were collected during autopsy, embedded in paraffin, sectioned, and stained with haematoxylin and eosin. Genomic DNA was isolated from tissue samples of abnormal foetuses and from blood leukocytes of their healthy siblings. PCR reactions were used to reveal the presence of Y-linked genes (SRY and AMELY) and an X-linked gene (AMELX).</p></sec>
<sec id="j_jvetres-2019-0057_s_007">
<h3>Results</h3>
<p>All foetuses were classified to the group<italic>holoacardius amorphous (anideus)</italic>. Molecular analysis clearly showed that at 17 microsatellite loci, the studied amorphous foetuses had identical genotypes to the viable co-twins.</p></sec>
<sec id="j_jvetres-2019-0057_s_008">
<h3>Conclusion</h3>
<p>Foetuses had monozygotic origin. Histological analysis showed a low level of development of tissues of meso- and ectodermal origin, as well as features of degrading patterns.</p></sec></abstract>

Introduction
A comprehensive description is presented of four novel cases ofamorphus globosus (ag) foetuses originating from multiple pregnancies of Polish Holstein cows.

Material and Methods
Four amorphic foetuses were delivered by three cows. Tissue samples were collected during autopsy, embedded in paraffin, sectioned, and stained with haematoxylin and eosin. Genomic DNA was isolated from tissue samples of abnormal foetuses and from blood leukocytes of their healthy siblings. PCR reactions were used to reveal the presence of Y-linked genes (SRY and AMELY) and an X-linked gene (AMELX).

Results
All foetuses were classified to the groupholoacardius amorphous (anideus). Molecular analysis clearly showed that at 17 microsatellite loci, the studied amorphous foetuses had identical genotypes to the viable co-twins.

Conclusion
Foetuses had monozygotic origin. Histological analysis showed a low level of development of tissues of meso- and ectodermal origin, as well as features of degrading patterns.

Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
A comprehensive description is presented of four novel cases ofamorphus globosus (ag) foetuses originating from multiple pregnancies of Polish...

Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies

Published Online: Sep 13, 2019

Page range: 391 - 398

Received: Aug 15, 2018

Accepted: Aug 07, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0057

Keywords
cattle, foetuses, histology, monozygotic pregnancy

© 2019 M. Gehrke et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies

Published Online: Sep 13, 2019

Page range: 391 - 398

Received: Aug 15, 2018

Accepted: Aug 07, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0057

Keywordscattle, foetuses, histology, monozygotic pregnancy

© 2019 M. Gehrke et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cattle, foetuses, histology, monozygotic pregnancy