Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

Open access

Abstract

Pluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

Arya D., Patni V. 2013. Micropropagation of Pluchea lan–ceolata (Oliver & Hiern.) - A potent anti-arthritic medicinal herb. Int. J. Med. Arom. Plants 3: 55-60.

Arya D., Patni V., Kant U. 2008. In vitro propagation and quercetin quantification in callus cultures of Rasna (Pluchea lanceolata Oliver & Hiern.). Indian J. Bio-technol. 7: 383-387.

Bernabe-Antonio A., Santacruz-Ruvalcaba F., Cruz-Sosa F. 2012. Effect of plant growth regulators on plant regeneration of Dioscorea remotiflora (Kunth) through nodal explants. Plant Growth Regul. 68: 293-301. DOI: 10.1007/s10725-012-9717-z.

Bhagwat D.P., Kharya M.D., Bani S., Kaul A., Kour K., Chauhan P.S., Suri K.A., Satti N.K. 2010. Immu-nosuppressive properties of Pluchea lanceolata leaves. Indian J. Pharmacol. 42: 21-26. DOI: 10.4103/0253-7613.62405. Faisal M., Ahmad N., Anis M. 2005. Shoot multiplica–tion in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tiss. Org. 80: 187-190. DOI: 10.1007/s11240-004-0567-x.

Huetteman C.A., Preece J.E. 1993. Thidiazuron: a po–tent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. 33: 105-119. DOI: 10.1007/BF01983223.

Inamdar J.A., Nataraj M., Mohan J.S.S., Subramanian R.B. 1990. Somatic embryogenesis from callus cultures of Crataeva nurvala Buch. Ham. Phyto- morphology 40: 319-322.

Kumar S., Narula A., Sharma M.P., Srivastava P.S. 2004. In vitro propagation of Pluchea lanceolata, a medicinal plant, and effect of heavy metals and different amino-purines on quercetin content. In vitro Cell. Dev. - Pl. 40: 171-176. DOI: 10.1079/IVP2003490.

Lu C-Y. 1993. The use of thidiazuron in tissue cul–ture. In vitro Cell. Dev. - Pl. 29: 92-96. DOI: 10.1007/BF02632259.

Murashige T., Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.x.

Murthy B.N., Murch S., Saxena P.K. 1998. Thidiazuron: A potent regulator of in vitro plant morpho–genesis. In vitro Cell. Dev. - Pl. 34: 267-275. DOI: 10.1007/BF02822732.

Noshad D., Miresmaili S., Riseman A., Ekramoddoul-lah A. 2009. In vitro propagation of seven Daphne L. species. Plant Cell Tiss. Org. 96: 201–209. DOI: 10.1007/s11240-008-9476-8.

Phulwaria M., Rai M.K., Harish, Gupta A.K., Ram K., Shekhawat N.S. 2012. An improved micro-propagation of Terminalia bellirica from nodal explants of mature tree. Acta Physiol. Plant. 34: 299-305. DOI: 10.1007/s11738-011-0828-3.

Phulwaria M., Shekhawat N.S., Rathore J.S., Singh R.P. 2013. An efficient in vitro regeneration and ex vitro rooting of Ceropegia bulbosa Roxb.-A threatened and pharmaceutical important plant of Indian Thar Desert. Ind. Crop. Prod. 42: 25–29. DOI: 10.1016/j.indcrop.2012.05.013.

Rathore M.S., Shekhawat N.S. 2013. Ex vivo implica–tions of phytohormones on various in vitro re–sponses in Leptadenia reticulata (Retz.) Wight. & Arn. - An endangered plant. Environ. Exp. Bot. 86: 86-93. DOI: 10.1016/j.envexpbot.2010.05.009.

Shekhawat M.S., Shekhawat N.S. 2011. Micropropaga–tion of Arnebia hispidissima (Lehm). DC. and pro–duction of alkannin from callus and cell suspension culture. Acta Physiol. Plant. 33: 1445-1450. DOI: 10.1007/s11738-010-0680-x.

Siddique I., Anis M. 2007. In vitro shoot multiplica–tion and plantlet regeneration from nodal ex–plants of Cassia angustifolia (Vahl.): a medicinal plant. Acta Physiol. Plant. 29: 233-238. DOI: 10.1007/s11738-007-0029-2.

Sivanesan I., Song J.Y., Hwang S.J., Jeong B.R. 2011. Mi–cropropagation of Cotoneaster wilsonii Nakai -a rare endemic ornamental plant. Plant Cell Tiss. Org. 105: 55-63. DOI: 10.1007/s11240-010-9841-2.

Srivastava V., Varma N., Tandon J., Srimal R. 1990. Anti-inflammatory activity of Pluchea lanceolata: isolation of an active principle. Pharm. Biol. 28: 135-137. DOI:10.3109/13880209009082796.

Tripathi M., Kumari N. 2010. Micropropagation of a tropical fruit tree Spondias mangifera Willd. through direct organogenesis. Acta Physiol. Plant. 32: 1011-1015. DOI: 10.1007/s11738-010-0484-z.

Varghese S.K., Inamdar J.A., Kalia K., Subramanian R.B., Nataraj M. 1993a. In vitro organogenesis in Enicostema hyssopifolium (Willd.) Verdoorn. Proc. Natl. Acad. Sci. India B 63: 219-221.

Varghese S.K., Inamdar J.A., Kalia K., Subramanian R.B., Nataraj M. 1993b. Micropropagation of Aegle marmelos (L.) Corr. Phytomorphology 43: 87-92.

Varghese S.K., Subramanian R.B., Nataraj M., Kalia K., Inamdar J.A. 1992. In vitro studies on Vitex negundo. Indian Botanical Contactor 9: 147-150.

Vasudevan R., van Staden J. 2011. Cytokinin and ex–plant types influence in vitro plant regeneration of Leopard Orchid (Ansellia africana Lindl.). Plant Cell Tiss. Org. 107: 123-129. DOI: 10.1007/s11240-011-9964-0.

Wood H.N., Braun A.C. 1961. Studies on the regulation of certain essential biosynthetic systems in normal and crown gall tumor cells. Proc. Natl. Acad. Sci. USA 47: 1907-1913.

Journal Information


CiteScore 2018: 0.51

SCImago Journal Rank (SJR) 2018: 0.207
Source Normalized Impact per Paper (SNIP) 2018: 0.497

Cited By

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 237 179 10
PDF Downloads 122 110 11