<abstract xmlns="http://www.w3.org/1999/xhtml"><italic>Pluchea lanceolata</italic> is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. <italic>lanceolata</italic> using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). <italic>In vitro</italic> raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.</abstract>

Pluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

Micropropagation of <italic>P<sc>LUCHEA LANCEOLATA</sc></italic> (Oliver &amp; Hiern.) Using Nodal Explant

B.R. Doshi School of Biosciences, Sardar Patel Maidan, Sardar Patel University Vadtal Road, Post Box No. 39, Vallabh Vidyanagar-388120, India

Department of Statistics Sardar Patel University Vallabh Vidyanagar 388 120, Gujarat, India

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Micropropagation of  (Oliver & Hiern.) Using Nodal Explant

Pluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established...

Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

Published Online: Sep 10, 2014

Page range: 35 - 39

Received: Oct 17, 2013

Accepted: Apr 16, 2014

DOI: https://doi.org/10.2478/johr-2014-0004

Keywords
micropropagation, TDZ, BAP, Kin, 2iP

© 2014 Mafatlal M. Kher et. al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

Published Online: Sep 10, 2014

Page range: 35 - 39

Received: Oct 17, 2013

Accepted: Apr 16, 2014

DOI: https://doi.org/10.2478/johr-2014-0004

Keywordsmicropropagation, TDZ, BAP, Kin, 2iP

© 2014 Mafatlal M. Kher et. al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
micropropagation, TDZ, BAP, Kin, 2iP