Influence of selected heavy metals (As, Cd, Cr, Cu) on nematode communities in experimental soil microcosm

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In this study, the effects of arsenic, cadmium, copper and chromium treatments were examined on a nematode community structure and proportion of functional groups in the microcosm for 30 days. The toxic effects on the nematode community did not correspond with metals mobility (EDTA extraction) in soil as it was expected. The most toxic element with a significant degradation of community structure was chromium (low mobile), which negatively affected almost all observed ecological parameters (abundance, diversity and ecological indices). On the other hand, cadmium and arsenic influence was negligible even in the plots treated with the highest concentrations and the communities resembled to the control samples. Copper showed a stimulative effect on the community under low concentration (40, while under higher concentrations the stimulation was replaced by stress responses. The widely used ecological indices, such as the Maturity Index 2-5, Structure Index, and Shannon-Weaver Index and c-p groups showed the best bioindication potential among nematode parameters.

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  • BAKONYI G. NAGY P. KÁDÁR I. (2003): Long-term effects of heavy metals and microelements on nematode assemblage. Toxicol.

  • Lett. 140 - 141: 391 - 401. DOI: 10.1016/S0378-4274(03)00035-3

  • BONGERS T. KORTHALS G.W. (1993) The Maturity Index an instrument to monitor changes in the nematode community structure. Summaries of the 45th International Symposium on Crop Protection. Ghent Belgium pp 80

  • BONGERS T. ILIEVA-MAKULEC K. EKSCHMITT K. (2001): Acute sensitivity of nematode taxa to CuSO4 and relationships with feeding type and life-history classifi cation. Environ. Toxicol. Chem. 20(7): 1511 - 1516. DOI: 10.1002/etc.5620200714

  • CHEN G. QIN J. SHI D. ZHANG Y. JI W. (2009): Diversity of soil nematodes in area polluted with heavy metals and polycyclic aromatic hydrocarbons (PAHs) in Lanzhou China. Environ. Manag. 44(1): 163 - 172. DOI: 10.1007/s00267-008-9268-2

  • FERRIS H. BONGERS T. DE GOEDE R.G.M. (2001): A framework for soil food web diagnostics: extension of the nematode faunal analysis concept. Appl. Soil Ecol. 18(1): 13 - 29. DOI: 10.1016/ S0929-1393(01)00152-4

  • GEORGIEVA S.S. MCGRATH S.P. HOOPER D.J. CHAMBERS B.S. (2002): Nematode communities under stress: the long-term effects of heavy metals in soil treated with sewage sludge. Appl. Soil Ecol. 20(1): 27 - 42. DOI: 10.1016/S0929-1393(02)00005-7

  • KABATA-PENDIAS A. (2011): Trace Elements in Soils and Plants (4th edition) CRC Press Taylor and Francis Group Boca Raton USA.

  • KIMPINSKI J. STURZ A.V. (2003): Managing crop root zone ecosystems for prevention of harmful and encouragement of benefi cial nematodes. Soil Till. Res. 72(2): 213 - 221. DOI: 10.1016/S0167-1987(03)00090-4

  • KORTHALS G.W. VAN DE ENDE A. VAN MEGEN H. LEXMOND T.M. KAMMENGA J.E. BONGERS T. (1996a) Short-term effects of cadmium copper nickel and zinc on soil nematodes from different feeding and life-history strategy groups. Appl. Soil Ecol. 4: 107-117. DOI: 10.1016/0929-1393(96)00113-8

  • KORTHALS G.W. ALEXIEV A.D. LEXMOND T.M. KAMMENGA J.E. BONGERS T. (1996b): Long-term effects of copper and pH on the nematode community in an agroecosystem. Environ. Toxicol. Chem. 15(6). DOI: 10.1002/etc.5620150621

  • KORTHALS G.W. POPOVICI I. ILIEV I. LEXMOND T.M. (1998): Infl uence of perennial ryegrass on a copper and zinc affected terrestrial nematode community. Appl. Soil Ecol. 10(1 - 2): 73 - 85. DOI: 10.1016/S0929-1393(98)00039-0

  • MARD (1994): Ministry of Agriculture and Rural Development Degree No. 531/1994-540. Retrieved from (In Slovak) MARD (2005): Ministry of Agriculture and Rural Development Degree No. 338/2005. Retrieved from (In Slovak) NAGY P. (1999): Effect of an artifi cial metal pollution on nematode assemblage of a calcareous loamy chernozem soil. Plant Soil 212: 35 - 43. DOI: 10.1023/A:1004657924496

  • NAGY P. BAKONYI G. BONGERS T. KÁDÁR I. FÁBIÁN M. KISS I. (2004): Effects of microelements on soil nematode assemblages seven years after contaminating an agricultural fi eld. Sci. Total Environ. 320(2 - 3): 131 - 143. DOI: 10.1016/j.scitotenv.2003.08.006

  • NEMAPLEX - available at internet: nemaplex/ - Accessed 25 June 2014.

  • PEN-MOURATOV S. SHUKUROV N. STEINBERGER Y. (2008): Infl uence of industrial heavy metal pollution on soil free-living nematode population. Environ. Pollut. 152(1): 172 - 183. doi:10.1016/j.envpol.2007.05.007

  • SABIENË N. BRAZAUSKIENË D.M. RIMMER D. (2004): Determination of heavy metal mobile forms by different extraction methods. Ekologija 1 36 - 41

  • ŠALAMÚN P. RENČO M. KUCANOVÁ E. BRÁZOVÁ T. PAPAJOVÁ I. MIKLISOVÁ D. HANZELOVÁ V. (2012): Nematodes as bioindicators of soil degradation due to heavy metals. Ecotoxicology 21(8): 2319 - 2330. DOI: 10.1007/s10646-012-0988-y

  • SCHWARTZ M.S BENCI J.L. SELOTE D.S. SHARMA A.K. CHEN A.G.Y. DANG H. FARES H. VATAMANIUK O.K. (2010): Detoxifi cation of multiple heavy metals by a half-molecule ABC transporter HMT-1 and Coelomocytes of Caenorhabditis elegans. PLoS One 5(3): e9654. DOI:10.1371/journal.pone.0009564

  • SCHULTZ E. JOUTTI A. (2007): Arsenic ecotoxicity in soils. Risk assessment and risk management procedure for arsenic in the tampere region. Finish Environment Institute.

  • SHANNON C.E. WEAVER W. (1949): Recent contribution to the mathematical theory of communication.1st ed. Urbana IL: University of Illinois Press.

  • SOUTHEY J.F. (1986): Laboratory methods for work with plant and soil nematodes. Norwich: H.M.S.O. Books.

  • STEWART M.A. JARDINE P.M. BRANDT C.C. BARNETT M.O. FENDORF S.E. MCKAY. L.D. MEHLHORN T.L. PAUL K. (2003): Effects of contaminant concentration aging and soil properties on the bioaccessibility of Cr(III) and Cr(VI) in soil. Soil Sediment Contam. 12(1): 1 - 21. DOI: 10.1080/713610958

  • TER BRAAK C.J.F. ŠMILAUER P. (2012): CANOCO reference manual and user’s guide: software for ordination version 5.0. Microcomputer Power Ithaca USA: Biometris. 496 p.

  • WILSON M.J. KAKOULI-DUARTE T. (2009): Nematodes as Environmental Indicators. Wallingford UK: CAB International.

  • YANG J.K. BARNETT M.O. JARDINE P.M. BASTA N.T. CASTEEL S.W. (2002): Adsorption sequestration and bioaccessibility of As(V) in soils. Environ. Sci. Technol. 36(21): 4562 - 4569. DOI: 10.1021/ es011507s

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