A screening method allowing the quantification of 24 aerosol constituents using gas chromatography-mass spectrometry has been developed to assess the aerosol chemistry of heat-not-burn tobacco products.
The aim of this method was to quantify phenol,
The method was validated according to the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use and the Association of Official Analytical Chemists guidelines.
A regression model based on a linear relationship between concentration and response ratio with a 1/x weighting factor was selected for phenol,
The matrix effect of the heated tobacco aerosol extract was assessed by performing a recovery study, where the aerosol extracts were spiked at different concentrations for the compounds to be analyzed. In addition, the mainstream smoke from 3R4F reference cigarettes was analyzed, and results were compared with previously published studies. The method was successfully validated, providing data consistent with published data and it was shown to be selective, precise and accurate.