Leptospirosis in Cattle From Markets of Almaty Province, Kazakhstan

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This paper is the first study of the prevalence of leptospirosis in the cattle at slaughter from a rural area of Kazakhstan. Five hundred and seventy three samples of serum, urine, and kidneys from cattle of Alatau, Kazakh white and Auliyekol breed, aged from 2 to 5 years (unknown vaccination status), from the province of Almaty in the South-Eastern region were collected during four years (March 2010 to October 2013). The serological, bacteriological, and molecular analyses were performed. Serum samples were tested with 14 reference Leptospira serovars by microscopic agglutination test (MAT). MAT results showed that 89 (15.53%) serum samples had detectable antibodies against seven serovars of L. interrogans at a dilution of ≥1:100. Serovars: Pomona (38.2%), Tarassovi (27.2%), and Kabula (18.8%) were the most prevalent and their titres ranged from 100 to 1200. The spirochetes were detected in 11 samples of urine and nine samples of kidneys under dark-field microscope observation. The pure cultures were obtained from three samples. PCR technique confirmed leptospirosis in 23 out of 89 urine samples from cows, which showed the presence of leptospiral antibodies in microagglutination test. The high disease prevalence in cows indicates the high Leptospira contamination in this area. It was concluded that the bovine leptospirosis is an endemic and locally widespread disease in Kazakhstan, and that it may play a role in zoonotic transmission to humans.

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  • 1. Bharti A.R. Nally J.E. Ricaldi J.N. Matthias M.A. Diaz M.M. Lovett M.A. Levett P.N. Gilman R.H. Willig M.R. Gotuzzo E.: Leptospirosis: a zoonotic disease of global importance. Lanc Infect Dis 2003 3 757–771.

  • 2. Bolin C.A.: Diagnosis and Control of Bovine Leptospirosis Proceedings of the 6th Western Dairy Management Conference Reno 2003 p. 155.

  • 3. Boqvist S. Montgomery J.M. Hurst M. Thu Ho Thi Viet Evengall E. Olsson Gunnarsson A. Magnusson U.: Leptospira in slaughtered fattening pigs in southern Vietnam: presence of the bacteria in kidneys and association with morphological findings. Vet Microbiol 2003 93 361–368.

  • 4. Carole A.B.: Leptospirosis in cattle: disease review and update. Proceeding of the NAVC North American Veterinary Conference Orlando Floryda 2005.

  • 5. Carpenter J.A. Scorgieb A. Josephson G.: Leptospira interrogans serovar Pomona infection associated with carcass condemnation of swine at slaughter. J Swine Health Prod 2006 14 145–148.

  • 6. Durham P.J.K. Paine G.D.: Serological survey for antibodies to infectious agents in beef cattle in Northern South Australia. Aust Vet J 1997 75 134–140.

  • 7. Engelen A.: Dairy development in Kazakhstan. FAO Rome 2011.

  • 8. Faine S. Adler B. Bolin C.A. Perolat P.: Leptospira and leptospirosis. MediSci Press Melbourne Australia 1999.

  • 9. Fearnley C. Wakley P.R. Gallego-Beltran J. Dalley C. Williamson S. Gaudie C. Woodward M.J.: The development of a real time PCR to detect pathogenic Leptospira species in kidney tissue. Res Vet Sci 2008 85 8–16.

  • 10. Flake L. Zharmagambetov Z.: Kazakhstan Outlines Continued Strategy and Support for Cattle Sector. USDA Forein Agricultural Service Global Information Network 2013 6 1–46.

  • 11. Haji Hajikolaei M.R. Ghorbanpour Najafabadi M. Abdollahpour G.: Serological study of leptospirosis in cattle in Ahvaz. J Fac Vet Med Univ Tehran 2005 60 7–14.

  • 12. Hernandez-Rodriguez P. Diaz C.A. Dalmau E.A. Quintero G.M.: A comparison between polymerase chain reaction (PCR) and traditional techniques for the diagnosis of leptospirosis in bovines. J Microbiol Meth 2011 84 1–7.

  • 13. Harkin K.R. Roshto Y.M. Sullivan J.T. Purvis T.J. Cheengappa M.A.: Comparison of polymerase chain reaction assay bacteriologic culture and serologic testing in assessment of prevalence of urinary shedding of leptospires in dogs. J Am Vet Med Assoc 2003 222 1230–1233.

  • 14. http://www.fao.org/docrep/009/ah759e/AH759E08.htm

  • 15. Il'yasov B.K: The etiological structure of Leptospirosis in the Republic of Kazakhstan (the epidemiological map). Copyright certificate number 365 of 17.07.1998.

  • 16. Karimuribo E.D. Swai E.S. Kyakaisho P.K.: Investigation of a syndrome characterised by passage of red urine in smallholder dairy cattle in East Usambara Mountains Tanzania. J S Afr Vet Assoc 2008 79 89–94.

  • 17. Kee S. Kim I. Choi M. Chang W.: Detection of leptospiral DNA by PCR. J Clin Microbiol 1994 32 1035–1039.

  • 18. Lilenbaum W. Souza G.N.: Factors associated with bovine leptospirosis in Rio de Janeiro. Brazil Res Vet Sci 2003 75 249–521.

  • 19. Malakhov Y.A. Panin A.N. Sobolev G.L.: Leptospirosis in farm animals. Moscow 2000 p. 420.

  • 20. Otaka D.Y. Martins G. Hamond C. Penna B. Medeiros M.A. Lilenbaum W.: Serology and PCR for bovine leptospirosis: herd and individual approaches. Veterinary Record published online 2012 March 16.

  • 21. Meites E. Jay M.T. Deresinski S. Shieh Wun-Ju. Zaki S.R. Tompkins L. Smith D.S.: Reemerging leptospirosis California. Emerg Infect Dis 2004 10 406–412.

  • 22. Mgode G.F. Machang'u R.S. Goris M.G. Engelbert M. Sondij S. Hartskeerl R.A.: New Leptospira serovar Sokoine of serogroup Icterohaemorrhagiae from cattle in Tanzania. Int J Syst Evol Micr 2006 56 (Pt 3) 593–977.

  • 23. Resolution of the Government of the Republic of Kazakhstan Astana Ukimet Uyi on July. 29 2011 No. 877 On approval of the comprehensive action plan for implementing the project “Development of export potential of cattle meat” for 2011–2015 http://kazpravda.kz/_pdf/oct11/221011decision.pdf

  • 24. Roach J.M. van Vuuren M. Picard J.A.: A serological survey of antibodies to Leptospira species in dogs in South Africa. J S Afr Vet Assoc 2010 81 156–159.

  • 25. Segura-Correa V.M. Solis-Calderon J.J. Segura-Correa J.C.: Seroprevalence of and risk factors for leptospiral antibodies among cattle in the state of Yucatan Mexico. Trop Anim Health Prod 2003 35 293–299.

  • 26. Statistical Yearbook of Kazakhstan 2009: www.stat.kz.

  • 27. Swai E.S. Schoonman L. Machang'u R.S.: Prevalence and factors associated with bovine leptospirosis in small scale dairy farms in Tanga region Tanzania. Bull Anim Health Prod Afr 2005 53 51–59.

  • 28. Tansuphasiri U. Chanthadee R. Phulsuksombati D. Sangjun N.: Development of a duplex–polymerase chain reaction for rapid detection of pathogenic Leptospira. South Asian J Tropic Med Pub Health 2006 37 297–308.

  • 29. Wasiński B. Sroka J. Wójcik-Fatla A. Zając V. Cisak E. Knap J. Sawczyn A. Dutkiewicz J.: Occurrence of leptospirosis in domestic animals reared on exposed or non-exposed to flood areas of eastern Poland. Bull Vet Inst Pulawy 2012 56 489–493.

  • 30. Zharmagambetova Z.: Agricultural Development Program 2013 –2020. USDA Foreign Agricultural Service Global Information Network 2013 pp. 1–80.

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