The chemical composition marble goby and sunflower meal is presented. The experimental results of enzymatic hydrolysis velocity in dependence of the protein concentration in substrate systems are set. Michaelis constants values for industrial proteolytic enzymes Corolase ® L10 and Corolase ® L7089 are calculated. The application experimental - theoretical kinetic analysis for protease / proteins congeniality determine in complex dispersion substrate systems expediency was confirmed. It is determined that microbial enzyme drag Corolase ® L7089 has a higher congeniality to proteins of all the tested substrates than plant enzyme drag Corolase ® L10
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