Effect of Level of Spirulina Supplementation on the Fatty Acid Compositions of Adipose, Muscle, Heart, Kidney and Liver Tissues in Australian Dual-Purpose Lambs

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This study investigated the effect of level of Spirulina supplementation on the fatty acid (FA ) compositions of subcutaneous adipose, longissimus dorsi muscle, kidney, heart, and liver tissues in purebred and crossbred Australian Merino sheep. Forty-eight lambs sired by Black Suffolk, White Suffolk, Dorset and Merino rams were assigned into 4 treatment groups of daily Spirulina supplementation levels per lamb of 0 mL (control), 50 mL (low), 100 mL (medium) and 200 mL (high) referred to as 0, 5, 10 and 20% groups. The lambs were slaughtered after 9 weeks of supplementation and heart, kidney, adipose, liver and muscle tissue samples were collected. The results demonstrated significant variations in growth and body conformation traits and tissue and organ FA composition in response to the Spirulina supplementation. The medium-level Spirulina treatment group increased the ω-3 and ω-6 polyunsaturated fatty acid (PUFA ) composition in all tissues and organs significantly. The results suggest the use of medium level (100 mL/head/day) of Spirulina supplementation in order to increase lamb production with more ω-3 and ω-6 PUFA and therefore higher nutritional meat quality.

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  • Abeywardena M.Y. Patten G.S. (2011). Role of omega-3 long-chain polyunsaturated fatty acids in reducing cardio-metabolic risk factors. Endocr. Metab. Immun. Disord. Drug Targets 22: 223-235.

  • Afolayan R.A. Adeyinka I.A. Lakpini C.A.M. (2006). The estimation of live weight from body measurements in Yankasa sheep. Czech J. Anim. Sci. 51: 343-348.

  • Alfaia C.P.M. Alves S.P. Martins S.I.V. Costa A.S.H. Fontes C.M.G.A. Lemos J.P.C. Bessa R.J.B. Prates J.A.M. (2009). Effect of the feeding system on intramuscular fatty acids and conjugated linoleic acid isomers of beef cattle with emphasis on their nutritional value and discriminatory ability. Food Chem. 114: 939-946.Belay A. Ota Y. Miyakawa K. Shimamatsu H. (1993). Current knowledge on potential health benefits of Spirulina. J. Appl. Phycol. 5: 235-241.

  • Bligh E.G. Dyer W.J. (1959). Arapid method of total lipid extraction and purification. Canadian J. Biochem. Physiol. 37: 911-917.

  • Cooper S.L. Sinclair L.A. Wilkinson R.G. Hallett K.G. Enser M. Wood J.D. (2004). Manipulation of theω-3 polyunsaturated fatty acid content of muscle and adipose tissue in lambs. J. Anim. Sci. 82: 1461-1470.

  • Daley C.A. Abbott A. Doyle P.S. Nader G.A. Larson S. (2010). Areview of fatty acid profiles and antioxidant content in grass-fed and grain-fed beef. Nutr. J. 9:10; doi:10.1186/1475-2891-9-10.

  • De Busk R. (2010). The role of nutritional genomics in developing an optimal diet for humans. Nutr. Clin. Prac. 25: 627-633.

  • Doreau M. Bauchart D. Chilliard Y. (2010). Enhancing fatty acid composition of milk and meat through animal feeding. Anim. Prod. Sci. 51: 19-29.

  • Fokkema M.R. Smit E.N. Martini I.A. Woltil H.A. Boersma E.R. Muskiet F.A.J. (2002). Assessment of essential fatty acid andω3-fatty acid status by measurement of erythrocyte 20:3ω9 (Mead acid) 22:5ω6/20:4ω6 and 22:5ω6/22:6ω3. Prostagland. Leukotri. Essen. Fatty Acids 67: 345-356.

  • Hoashi S. Hinenoya T. Tanaka A. Ohsaki H. Sasazaki S. Taniguchi M. Oyam a K. Mukai F. Mannen H. (2008). Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese Black cattle. BMC Genet. 9 p. 84; doi:10.1186/1471-2156-9-84; http://www.biomedcentral.com/1471-2156/9/84

  • Holman B.W.B. Kashani A. Malau- Aduli A.E.O. (2014 a). Effects of Spirulina (Arthrospira latensis) supplementation level and basal diet on liveweight body conformation and growth traits in genetically divergent Australian dual-purpose lambs during simulated drought and typical pasture grazing. Small Rum. Res. 120: 6-14; doi http://dx.doi.org/10.1016/j.smallrumres.2014.04.014

  • Holman B.W.B. Kashani A. Malau- Aduli A.E.O. (2014 b). Wool quality traits of purebred and crossbred Merino lambs orally drenched with Spirulina (Arthrospira platensis). Italian J. Anim. Sci. 13: 387-391; doi: 10.4081/ijas.2014.3174.

  • Holman B.W.B. Flakemore A.R. Kashani A. Malau- Aduli A.E.O. (2014 c). Spirulina supplementation sire breed sex and basal diet effects on lamb intramuscular fat percentage and fat melting points. Int. J. Vet. Med. Res. Rep. 2014 Article ID 263951; doi: 10.5171/2014.263951 9 pp.

  • Holman B.W.B. Malau- Aduli A.E.O. (2014). Effects of Spirulina supplementation on wool quality in purebred and crossbred Merino lambs fed pasture and lucerne hay basal diets. J. Agric. Sci. 6: 120-127; doi: 10.5539/jas.v6n7p120.

  • Holman B.W.B. Malau- Aduli A.E.O. (2013). Spirulina asalivestock supplement and animal feed. J. Anim. Physiol. Anim. Nutr. 97: 615-623; doi: 10.1111/j.1439-0396.2012.01328.x.

  • Holman B.W.B. Kashani A. Malau- Aduli A.E.O. (2012). Growth and body conformation responses of genetically divergent Australian sheep to Spirulina (Arthrospira platensis) supplementation. American J. Expt. Agric. 2: 160-173.

  • Iwata K. Inayama T. Kato T. (1990). Effects of Spirulina platensis on plasma lipoprotein lipase activity in fructose-induced hyperlipidemic rats. J. Nutri. Sci. Vit. 36: 165-171.

  • Kashani A. Holman B.W.B. Nichols P.D. Malau- Aduli A.E.O. (2015). Effect of dietary supplementation with Spirulina on the expression of AANAT ADRB3 BTG2 and FASNgenes in the subcutaneous adipose and Longissimus dorsi muscle tissues of purebred and crossbred Australian sheep. J. Anim. Sci. Tech. 57 p. 8; doi:10.1186/s40781-015-0047-3.

  • Kouba M. Mourot J. (2011). Areview of nutritional effects on fat composition of animal products with special emphasis onω-3 polyunsaturated fatty acids. Biochimie 93: 13-17.

  • Mapiye C. Chimonyo M. Dzama K. Hugo A. Strydom P.E. Muchenje V. (2011). Fatty acid composition of beef from Nguni steers supplemented with Acacia karroo leaf-meal. J. Food Comp. Anal. 34: 556-567.

  • Mc Leod B.M. White A.K. O’ Halloran W.J. (2010). Marketing of sheep and sheep meat. In: International Sheep and Wool Handbook D.J. Cottle (Ed.) Nottingham University Press: Nottingham UK pp. 677-690.Miller M.R. Nichols P.D. Barnes J. Davies N.W. Peacock E.J. Carter C.G. (2006).

  • Regiospecificity profiles of storage and membrane lipids from the gill and muscle tissue of Atlantic salmon (Salmo salar L.) grown at elevated temperature. Lipids 41: 865-876.

  • Moibi J.A. Christopherson R.J. (2001). Effect of environmental temperature andaprotected lipid supplement on the fatty acid profile of ovine longissimus dorsi muscle liver and adipose tissues. Livest. Prod. Sci. 69: 245-254.

  • Nguyen N.H. Ponzoni R.W. Yee H.Y. Abu- Bakar K.R. Hamzah A. Khaw H.L. (2010). Quantitative genetic basis of fatty acid composition in the GIFTstrain of Nile tilapia (Oreochromis niloticus) selected for high growth. Aquaculture 309: 66-74.

  • Pethick D.W. Ball A.J. Banks R.G. Hocquette J.F. (2010). Current and future issues facing red meat quality inacompetitive market and how to manage continuous improvement. Anim. Prod. Sci. 51: 13-18.

  • Phythian C.J. Hughes D. Michalopoulou E. Cripps P.J. Duncan J.S. (2012). Reliability of body condition scoring of sheep for cross-farm assessments. Small Rum. Res. 104: 156-162.

  • Price P.T. Nelson C.M. Clarke S.D. (2000). Omega-3 polyunsaturated fatty acid regulation of gene expression. Current Opin. Lipidol. 11: 3-7.

  • Qureshi M.A. Garlich J.D. Kidd M.T. (1996). Dietary Spirulina platensis enhances humoral and cell-mediated immune functions in chickens. Immunopharmacol. Immunotoxicol. 18: 465-476.

  • Raes K. De Smet S. Demeyer D. (2004). Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb beef and pork meat: Areview. Anim. Feed Sci. Tech. 113: 199-221.

  • Ross E. Dominy W. (1990). The nutritional value of dehydrated blue-green algae (Spirulina platensis) for poultry. Poultry Sci. 69: 794-800.

  • Rowe J.B. (2010). The Australian sheep industry - undergoing transformation. Anim. Prod. Sci. 50: 991-997.

  • Santos-Silva J. Bessa R.J.B. Santos-Silva F. (2002). Effect of genotype feeding system and slaughter weight on the quality of light lambs: II. Fatty acid composition of meat. Livest. Prod. Sci. 77: 187-194.

  • Scollan N.D. Choi N.J. Kurt E. Fisher A.V. Enser M. Wood J.D. (2001). Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle. British. J. Nutr. 85: 115-124.

  • Smet S.D. Raes K. Demeyer D. (2004). Meat fatty acid composition as affected by fatness and genetic factors:areview. Anim. Res. 53: 81-98.

  • Sowande O.S. Sobola O.S. (2008). Body measurements of West African dwarf sheep as parameters for estimation of liveweight. Trop. Anim. Hlth. Prod. 40: 433-439.

  • Wachira A.M. Sinclair L.A. Wilkinson R.G. Enser M. Wood J.D. Fisher A.V. (2002). Effects of dietary fat source and breed on the carcass composition ω-3 polyunsaturated fatty acid and conjugated linoleic acid content of sheep meat and adipose tissue. Brit. J. Nutr. 88: 697-709.

  • Wood J.D. Enser M. (1997). Factors influencing fatty acids in meat and the role of antioxidants in improving meat quality. Brit. J. Nutr. 78 (Suppl. 1): S49-S60.

  • Wood J.D. Enser M. Fisher A.V. Nute G.R. Sheard P.R. Richardson R.I. Hug- hes S.I. Whittington F.M. (2008). Fat deposition fatty acid composition and meat quality: Areview. Meat Sci. 78: 343-358.

  • Wood J.D. Richardson R.I. Nute G.R. Fisher A.V. Campo M.M. Kasapidou E. Sheard P.R. Enser M. (2004). Effects of fatty acids on meat quality:areview. Meat Sci. 66: 21-32.

  • Woods V.B. Fearon A.M. (2009). Dietary sources of unsaturated fatty acids for animals and their transfer into meat milk and eggs: Areview. Livest. Sci. 126: 1-20.

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