Antibiotic resistance profile and random amplification typing of β-lactamase-producing Enterobacteriaceae from the local area of Al-Taif and nearby cities in Saudi Arabia
Article Category: Original article
Published Online: Jan 31, 2017
Page range: 219 - 228
DOI: https://doi.org/10.5372/1905-7415.1003.483
Keywords
© 2016 Ali K. Alzahrani, Mostafa M. Farag, Said H. Abbadi, Mohamed M. Hassan, Ahmed Gaber, Ahmed S. Abdel-Moneim
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Background
The emergence of extended-spectrum β-lactamase (ESBL)-producing
Objectives
To screen the antibiotic susceptibility of
Methods
We analyzed 43 bacterial strains isolated from clinical samples of sporadic cases for their reactivity to different antibiotics. Strains that showed resistance by monoplex PCR were screened for ESBL TEM- and CTXM-1-, -2-, and -9-encoding genes. Random amplifications were used for typing the resistant strains.
Results
The majority of the ESBL containing strains were sensitive to meropenem, amikacin, gentamicin, and tigecycline. PCR detection using ESBL gene-specific primers showed that 17 of 43 strains harbored genes for ESBL TEM, CTX-M-1, or CTX-M-2. RAPD typing revealed marked variation among the ESBL-producing
Conclusions
Considerably high incidence of ESBL-producing bacteria was present patients from the local area of Al-Taif and nearby cities in Saudi Arabia with TEM and CTX-M subtypes being the most commonly detected variants. There was evidence of a polymorphic genetic pattern among ESBL-producing bacteria.