A review of the fauna of leaf-mining flies (Diptera: Agromyzidae) in the Gemer region (Slovakia) is presented. A total of 140 species of Agromyzidae are recorded from the Gemer region in central Slovakia (Muránska planina NP - 126 species, Slovenský raj NP - 54 species, Slovenský kras NP - 22 species). Three new species, Cerodontha (Butomomyza) gemerensis sp. n., Chromatomyiacepelaki sp. n. and Liriomyza muranica sp. n., are described from Slovakia (Gemer region). All descriptions are supplemented by illustrations of diagnostic features including those of the male and female terminalia. Twenty-one species, Cerodontha (Dizygomyza) luzulae (Groschke, 1957), Hexomyza simplicoides (Hendel, 1920), Liriomyza scorzonerae Rydén, 1951, L. urophorina Mik, 1894, Napomyza bellidis Griffiths, 1967, N. cichorii Spencer, 1966, N. maritima von Tschirnhaus, 1981, Ophiomyia spenceri Černý, 1985, O. stenophaga Pakalniškis, 1998, Phytomyza adjuncta Hering, 1928, P. brischkei Hendel, 1922, P. griffithsi Spencer, 1963, P. medicaginis Hering, 1925, P.podagrariae Hering, 1954, P. rydeni Hering, 1934, P. salviae (Hering, 1924), P. soenderupi Hering, 1941, P. spoliata Strobl, 1906, P. trolliivora Hering, 1935, P. tussilaginis Hendel, 1925 and P.vitalbae Kaltenbach, 1872, are recorded from Slovakia for the first time. The significance of the regional fauna of Agromyzidae in the Gemer area is discussed.
