<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2020-0003_s_005">
<h3>Introduction</h3>
<p>Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008–2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent <italic>M. bovis</italic>/<italic>caprae</italic> strains and determination of the epidemiological relationship of various bTB outbreaks.</p></sec>
<sec id="j_jvetres-2020-0003_s_006">
<h3>Material and Methods</h3>
<p>The material for microbiological tests came from 119 cattle (<italic>Bos taurus</italic>) from nine herds located in five provinces, neighbouring the Masovian Province.</p></sec>
<sec id="j_jvetres-2020-0003_s_007">
<h3>Results</h3>
<p>Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the <italic>Mycobacterium bovis</italic> species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains.</p></sec>
<sec id="j_jvetres-2020-0003_s_008">
<h3>Conclusion</h3>
<p>Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.</p></sec>
</abstract>

Introduction
Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008–2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent M. bovis/caprae strains and determination of the epidemiological relationship of various bTB outbreaks.

Material and Methods
The material for microbiological tests came from 119 cattle (Bos taurus) from nine herds located in five provinces, neighbouring the Masovian Province.

Results
Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the Mycobacterium bovis species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains.

Conclusion
Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.

Molecular characterisation of the <italic>Mycobacterium bovis</italic> causing bovine tuberculosis outbreaks in Poland

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular characterisation of the  causing bovine tuberculosis outbreaks in Poland

Introduction
Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there...

Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland

Data publikacji: 20 sty 2020

Zakres stron: 45 - 50

Otrzymano: 23 maj 2019

Przyjęty: 13 sty 2020

DOI: https://doi.org/10.2478/jvetres-2020-0003

Słowa kluczowe
cattle, bovine tuberculosis, Poland

© 2020 M. Krajewska-Wędzina et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland

Data publikacji: 20 sty 2020

Zakres stron: 45 - 50

Otrzymano: 23 maj 2019

Przyjęty: 13 sty 2020

DOI: https://doi.org/10.2478/jvetres-2020-0003

Słowa kluczowecattle, bovine tuberculosis, Poland

© 2020 M. Krajewska-Wędzina et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
cattle, bovine tuberculosis, Poland