<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Endophytic fungi of selected Nigerian plants are important sources of bioactive products with enormous potentials for the discovery of new drug molecules for drug development. <italic>Pseudomonas aeruginosa</italic> is one of the major causes of healthcare-associated bacterial infections, leading to increased mortality and morbidity. In this study, isolated endophytic fungi from <italic>Cola nitida</italic> were screened for anti-pseudomonas properties. Endophytic fungi associated with healthy leaves of <italic>C. nitida</italic> were isolated using standard methods. Fungi were identified through their morphological, cultural and microscopic characteristics. The fungi were subjected to solid-state fermentation and secondary metabolites extracted using ethyl acetate and concentrated under vacuum. The crude extracts were screened for antimicrobial activity against clinical and laboratory strains of <italic>Pseudomonas aeruginosa</italic> using the agar diffusion method. The bioactive components of the fungal extracts were identified using High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) analysis. Three endophytic fungi; <italic>Acremonium</italic> sp., <italic>Aspergillus</italic> sp. and <italic>Trichophyton</italic> sp. were isolated. At 1 mg/ml, extracts of the three fungi displayed antipseudomonal activity with inhibition zone diameter ranging from 6 - 4 mm. HPLC-DAD analysis revealed the presence of compounds, such as 4-hydroxyphenyl acetate. indole-3-acetic acid, and protocatechuic acid among others in the fungal extracts. The findings in this study reveal that endophytic fungi associated with <italic>C. nitida</italic> possess promising antipseudomonal properties. This finding can open new doors for the discovery of new agents against <italic>P. aeruginosa</italic>.</p></abstract>

Endophytic fungi of selected Nigerian plants are important sources of bioactive products with enormous potentials for the discovery of new drug molecules for drug development. Pseudomonas aeruginosa is one of the major causes of healthcare-associated bacterial infections, leading to increased mortality and morbidity. In this study, isolated endophytic fungi from Cola nitida were screened for anti-pseudomonas properties. Endophytic fungi associated with healthy leaves of C. nitida were isolated using standard methods. Fungi were identified through their morphological, cultural and microscopic characteristics. The fungi were subjected to solid-state fermentation and secondary metabolites extracted using ethyl acetate and concentrated under vacuum. The crude extracts were screened for antimicrobial activity against clinical and laboratory strains of Pseudomonas aeruginosa using the agar diffusion method. The bioactive components of the fungal extracts were identified using High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) analysis. Three endophytic fungi; Acremonium sp., Aspergillus sp. and Trichophyton sp. were isolated. At 1 mg/ml, extracts of the three fungi displayed antipseudomonal activity with inhibition zone diameter ranging from 6 - 4 mm. HPLC-DAD analysis revealed the presence of compounds, such as 4-hydroxyphenyl acetate. indole-3-acetic acid, and protocatechuic acid among others in the fungal extracts. The findings in this study reveal that endophytic fungi associated with C. nitida possess promising antipseudomonal properties. This finding can open new doors for the discovery of new agents against P. aeruginosa.

Screening of endophytic fungal metabolites from <italic>Cola nitida</italic> leaves for antimicrobial activities against clinical isolates of <italic>Pseudomonas aeruginosa</italic>

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Screening of endophytic fungal metabolites from  leaves for antimicrobial activities against clinical isolates of 

Endophytic fungi of selected Nigerian plants are important sources of bioactive products with enormous potentials for the discovery of new drug molecules for...

Screening of endophytic fungal metabolites from Cola nitida leaves for antimicrobial activities against clinical isolates of Pseudomonas aeruginosa

Article Category: Research Article

Data publikacji: 19 lip 2020

Zakres stron: 161 - 166

DOI: https://doi.org/10.2478/ebtj-2020-0019

Słowa kluczowe
Endophytic fungi, antimicrobial activity, secondary metabolites,

© 2020 David C. Nwobodo, Chibueze P. Ihekwereme, Festus B. C. Okoye, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Screening of endophytic fungal metabolites from Cola nitida leaves for antimicrobial activities against clinical isolates of Pseudomonas aeruginosa

Article Category: Research Article

Data publikacji: 19 lip 2020

Zakres stron: 161 - 166

DOI: https://doi.org/10.2478/ebtj-2020-0019

Słowa kluczoweEndophytic fungi, antimicrobial activity, secondary metabolites,

© 2020 David C. Nwobodo, Chibueze P. Ihekwereme, Festus B. C. Okoye, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Słowa kluczowe
Endophytic fungi, antimicrobial activity, secondary metabolites,