<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Animal lipoxygenases (EC 1.13.11.12, LOX) convert arachidonic acid to biological active compounds known as eicosanoids. Proinflammatory
eicosanoids - leukotrienes are involved in progression of atherosclerosis. Our attention was focused on a connection
between activity of LOX and primary hypertriacylglycerolaemia, untreated or treated with fenofibrate. Our results show that
LOX activity in the cytoplasm derived from liver of hypertriacylglycerolaemic rats is significantly increased (pH 7,2; relative to the
control group), which may be one reason for more rapid atherosclerosis progression in lipid metabolism disorders. The highest
LOX activity in microsomes was showed in groups of rats treated with fenofibrate. It appears that fibrates indirectly promote association
of LOXs to membranes. In fat fraction no significant effect of hypertriacylglycerolaemia on the activity of lipoxygenase
was found. Using gel electrophoresis significantly different spectrum of proteins was discovered in the control samples and
samples of hypertriacylglycerolaemic rats. It appears that in condition of lipid metabolism imbalance formation of proteins with
low molecular weight (and possibly also expression of LOX) is elevated.</p></abstract>

Animal lipoxygenases (EC 1.13.11.12, LOX) convert arachidonic acid to biological active compounds known as eicosanoids. Proinflammatory
eicosanoids - leukotrienes are involved in progression of atherosclerosis. Our attention was focused on a connection
between activity of LOX and primary hypertriacylglycerolaemia, untreated or treated with fenofibrate. Our results show that
LOX activity in the cytoplasm derived from liver of hypertriacylglycerolaemic rats is significantly increased (pH 7,2; relative to the
control group), which may be one reason for more rapid atherosclerosis progression in lipid metabolism disorders. The highest
LOX activity in microsomes was showed in groups of rats treated with fenofibrate. It appears that fibrates indirectly promote association
of LOXs to membranes. In fat fraction no significant effect of hypertriacylglycerolaemia on the activity of lipoxygenase
was found. Using gel electrophoresis significantly different spectrum of proteins was discovered in the control samples and
samples of hypertriacylglycerolaemic rats. It appears that in condition of lipid metabolism imbalance formation of proteins with
low molecular weight (and possibly also expression of LOX) is elevated.

Lipoxygenase in Condition of Experimental
Hypertriacylglycerolaemia

Department of Cell and Molecular Biology of Drugs, Faculty
of Pharmacy, Comenius University, Bratislava, Slovakia

European Pharmaceutical Journal

{"article-title":"Lipoxygenase in Condition of Experimental\nHypertriacylglycerolaemia"}

Animal lipoxygenases (EC 1.13.11.12, LOX) convert arachidonic acid to biological active compounds known as eicosanoids. Proinflammatory
eicosanoids -...

Lipoxygenase in Condition of Experimental Hypertriacylglycerolaemia

Data publikacji: 27 cze 2013

Zakres stron: 21 - 25

DOI: https://doi.org/10.2478/afpuc-2013-0011

This content is open access.